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Literature summary for 2.3.1.288 extracted from

  • Kumar, P.; Schelle, M.W.; Jain, M.; Lin, F.L.; Petzold, C.J.; Leavell, M.D.; Leary, J.A.; Cox, J.S.; Bertozzi, C.R.
    PapA1 and PapA2 are acyltransferases essential for the biosynthesis of the Mycobacterium tuberculosis virulence factor sulfolipid-1 (2007), Proc. Natl. Acad. Sci. USA, 104, 11221-11226 .
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 palmitoyl-CoA pH 7.5, 25°C Mycobacterium tuberculosis
2.5
-
 2-O-sulfo-alpha,alpha-trehalose pH 7.5, 25°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WIK7
-
-
Mycobacterium tuberculosis ATCC 25618 P9WIK7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + 2-O-sulfo-alpha,alpha-trehalose
-
 Mycobacterium tuberculosis 2-O-sulfo-2'-palmitoyl-alpha,alpha-trehalose + CoA
-
 ?
palmitoyl-CoA + 2-O-sulfo-alpha,alpha-trehalose
-
 Mycobacterium tuberculosis ATCC 25618 2-O-sulfo-2'-palmitoyl-alpha,alpha-trehalose + CoA
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0032
-
 palmitoyl-CoA pH 7.5, 25°C Mycobacterium tuberculosis
0.0067
-
 2-O-sulfo-alpha,alpha-trehalose pH 7.5, 25°C Mycobacterium tuberculosis

General Information

General Information Comment Organism
physiological function acyltransferases PapA2 and PapA1 are responsible for the sequential acylation of trehalose-2-sulfate to form diacetylated intermediate SL1278 and are essential for sulfolipid SL-1 biosynthesis. In vitro, recombinant PapA2 converts T2S to 2'-palmitoyl T2S, and PapA1 further elaborates this SL-1 intermediate to an analog of SL1278. Disruption of PapA2 and PapA1 genes results in loss of SL-1 (and SL1278). The deletions do not appear to affect bacterial replication, trafficking or virulence Mycobacterium tuberculosis