Literature summary for 2.3.1.286 extracted from

Zhao, K.; Chai, X.; Marmorstein, R.
Structure and substrate binding properties of cobB, a Sir2 homolog protein deacetylase from Escherichia coli (2004), J. Mol. Biol., 337, 731-741 .

Search for more literature for 2.3.1.286

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) and B834 (DE3) cells	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
native and selenomethionine-derivatized enzyme in complex with an 11-residue peptide containing residues 12-19 of histone H4 and acetylated at lysine 16, hanging drop vapor diffusion method, using	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	the small domain contains a structural zinc ion	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P75960	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q-Sepharose column chromatography and Superdex 75 gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[Acs protein]-N6-acetyl-L-lysine609 + NAD+ + H2O	-	Escherichia coli	[Acs protein]-L-lysine609 + 2''-O-acetyl-ADP-D-ribose + nicotinamide	-	?
[histone H4]-N6-acetyl-L-lysine16 + NAD+ + H2O	KGGAAc-KRHRKIL	Escherichia coli	[histone H4]-L-lysine16 + 2''-O-acetyl-ADP-D-ribose + nicotinamide	-	?
[p53 peptide]-N6-acetyl-L-lysine382 + NAD+ + H2O	-	Escherichia coli	[p53 peptide]-L-lysine382 + 2''-O-acetyl-ADP-D-ribose + nicotinamide	-	?

Synonyms

Synonyms	Comment	Organism
CobB	-	Escherichia coli
Sir2	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Escherichia coli

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Release 2023.1 (January 2023)