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Literature summary for 2.3.1.286 extracted from

  • Zhu, A.Y.; Zhou, Y.; Khan, S.; Deitsch, K.W.; Hao, Q.; Lin, H.
    Plasmodium falciparum Sir2A preferentially hydrolyzes medium and long chain fatty acyl lysine (2012), ACS Chem. Biol., 7, 155-159 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with [histone H3]-N6-myristoyl-L-lysine9, hanging drop vapor diffusion method, using 16% (w/v) PEG 3350, 0.1 M NaF, 7% (v/v) formamide Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
[histone H3]-N6-myristoyl-L-lysine9 Km below 0.001 mM, at pH 8.0 and 37°C Plasmodium falciparum
0.0012
-
[histone H3]-N6-octanoyl-L-lysine9 at pH 8.0 and 37°C Plasmodium falciparum
0.008
-
[histone H3]-N6-butyryl-L-lysine9 at pH 8.0 and 37°C Plasmodium falciparum
0.039
-
[histone H3]-N6-acetyl-L-lysine9 at pH 8.0 and 37°C Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q8IE47
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity column chromatography and Superdex 75 gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme preferentially hydrolyzes medium and long chain fatty acyl lysine. The deacetylase activity of the enzyme is weak Plasmodium falciparum ?
-
?
[histone H3]-N6-acetyl-L-lysine9 + NAD+ + H2O least efficient substrate Plasmodium falciparum [histone H3]-L-lysine9 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
?
[histone H3]-N6-butyryl-L-lysine9 + NAD+ + H2O
-
Plasmodium falciparum [histone H3]-L-lysine9 + 2''-O-butyryl-ADP-D-ribose + nicotinamide
-
?
[histone H3]-N6-myristoyl-L-lysine9 + NAD+ + H2O best substrate Plasmodium falciparum [histone H3]-L-lysine9 + 2''-O-myristoyl-ADP-D-ribose + nicotinamide
-
?
[histone H3]-N6-octanoyl-L-lysine9 + NAD+ + H2O
-
Plasmodium falciparum [histone H3]-L-lysine9 + 2''-O-octanoyl-ADP-D-ribose + nicotinamide
-
?

Synonyms

Synonyms Comment Organism
protein lysine deacetylase
-
Plasmodium falciparum
Sir2A
-
Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
[histone H3]-N6-acetyl-L-lysine9 at pH 8.0 and 37°C Plasmodium falciparum
0.001
-
[histone H3]-N6-butyryl-L-lysine9 at pH 8.0 and 37°C Plasmodium falciparum
0.001
-
[histone H3]-N6-octanoyl-L-lysine9 at pH 8.0 and 37°C Plasmodium falciparum
0.01
-
[histone H3]-N6-myristoyl-L-lysine9 Km below 0.001 mM, at pH 8.0 and 37°C Plasmodium falciparum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Plasmodium falciparum

General Information

General Information Comment Organism
physiological function the enzyme regulates the expression of surface antigens to evade the detection by host immune surveillance by removing medium and long chain fatty acyl groups from protein lysine residues Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.026
-
[histone H3]-N6-acetyl-L-lysine9 at pH 8.0 and 37°C Plasmodium falciparum
0.16
-
[histone H3]-N6-butyryl-L-lysine9 at pH 8.0 and 37°C Plasmodium falciparum
0.92
-
[histone H3]-N6-octanoyl-L-lysine9 at pH 8.0 and 37°C Plasmodium falciparum
10
-
[histone H3]-N6-myristoyl-L-lysine9 Km above 10 1/sec*mM, at pH 8.0 and 37°C Plasmodium falciparum