Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
in complex with [histone H3]-N6-myristoyl-L-lysine9, hanging drop vapor diffusion method, using 16% (w/v) PEG 3350, 0.1 M NaF, 7% (v/v) formamide | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
[histone H3]-N6-myristoyl-L-lysine9 | Km below 0.001 mM, at pH 8.0 and 37°C | Plasmodium falciparum | |
0.0012 | - |
[histone H3]-N6-octanoyl-L-lysine9 | at pH 8.0 and 37°C | Plasmodium falciparum | |
0.008 | - |
[histone H3]-N6-butyryl-L-lysine9 | at pH 8.0 and 37°C | Plasmodium falciparum | |
0.039 | - |
[histone H3]-N6-acetyl-L-lysine9 | at pH 8.0 and 37°C | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8IE47 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-affinity column chromatography and Superdex 75 gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme preferentially hydrolyzes medium and long chain fatty acyl lysine. The deacetylase activity of the enzyme is weak | Plasmodium falciparum | ? | - |
? | |
[histone H3]-N6-acetyl-L-lysine9 + NAD+ + H2O | least efficient substrate | Plasmodium falciparum | [histone H3]-L-lysine9 + 2''-O-acetyl-ADP-D-ribose + nicotinamide | - |
? | |
[histone H3]-N6-butyryl-L-lysine9 + NAD+ + H2O | - |
Plasmodium falciparum | [histone H3]-L-lysine9 + 2''-O-butyryl-ADP-D-ribose + nicotinamide | - |
? | |
[histone H3]-N6-myristoyl-L-lysine9 + NAD+ + H2O | best substrate | Plasmodium falciparum | [histone H3]-L-lysine9 + 2''-O-myristoyl-ADP-D-ribose + nicotinamide | - |
? | |
[histone H3]-N6-octanoyl-L-lysine9 + NAD+ + H2O | - |
Plasmodium falciparum | [histone H3]-L-lysine9 + 2''-O-octanoyl-ADP-D-ribose + nicotinamide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
protein lysine deacetylase | - |
Plasmodium falciparum |
Sir2A | - |
Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
[histone H3]-N6-acetyl-L-lysine9 | at pH 8.0 and 37°C | Plasmodium falciparum | |
0.001 | - |
[histone H3]-N6-butyryl-L-lysine9 | at pH 8.0 and 37°C | Plasmodium falciparum | |
0.001 | - |
[histone H3]-N6-octanoyl-L-lysine9 | at pH 8.0 and 37°C | Plasmodium falciparum | |
0.01 | - |
[histone H3]-N6-myristoyl-L-lysine9 | Km below 0.001 mM, at pH 8.0 and 37°C | Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Plasmodium falciparum |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme regulates the expression of surface antigens to evade the detection by host immune surveillance by removing medium and long chain fatty acyl groups from protein lysine residues | Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
[histone H3]-N6-acetyl-L-lysine9 | at pH 8.0 and 37°C | Plasmodium falciparum | |
0.16 | - |
[histone H3]-N6-butyryl-L-lysine9 | at pH 8.0 and 37°C | Plasmodium falciparum | |
0.92 | - |
[histone H3]-N6-octanoyl-L-lysine9 | at pH 8.0 and 37°C | Plasmodium falciparum | |
10 | - |
[histone H3]-N6-myristoyl-L-lysine9 | Km above 10 1/sec*mM, at pH 8.0 and 37°C | Plasmodium falciparum |