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Literature summary for 2.3.1.286 extracted from

  • Rumpf, T.; Gerhardt, S.; Einsle, O.; Jung, M.
    Seeding for sirtuins: microseed matrix seeding to obtain crystals of human Sirt3 and Sirt2 suitable for soaking (2015), Acta Crystallogr. Sect. F, 71, 1498-1510.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.286

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) CodonPlus RIPL cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with ADP ribose, microseed matrix seeding, using 17.5% (w/v) PEG 10000, 0.1 M ammonium acetate in 0.1 M bis-Tris buffer pH 6.8 at 20°C Homo sapiens
in complex with ADP ribose, microseed matrix seeding, using 25% (w/v) PEG 3350, 0.2 M MgCl2 in 0.1 M bis-Tris buffer pH 5.5 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains Zn2+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + [protein]-N6-acyl-L-lysine Homo sapiens
-
 nicotinamide + [protein]-L-lysine + 2'-O-acyl-ADP-ribose
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8IXJ6
-
-
Homo sapiens Q9NTG7
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex S75 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + [protein]-N6-acyl-L-lysine
-
 Homo sapiens nicotinamide + [protein]-L-lysine + 2'-O-acyl-ADP-ribose
-
 ?

Synonyms

Synonyms Comment Organism
SIRT2
-
 Homo sapiens
SIRT3
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Homo sapiens