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Literature summary for 2.3.1.28 extracted from
- Chloramphenicol acetyltransferase, a cytoplasmic protein is incompatible for export from Bacillus subtilis (1993), J. Bacteriol., 175, 5697-5700.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | Bacillus subtilis cells expressing a hybrid protein (LvsSS-Cat) consisting of the Bacillus amyloliquefaciens levansucrose signal peptide fused to Bacillus pumilus chloramphenicol acetyltransferase are unable to export cat protein into the growth medium. A series of tripartite protein fusion is constructed by inserting various length of the cat sequences between the levansucrase signal peptide and staphylococcal protein A or Escherichia coli alkaline phosphatase. Biochemical characterization of the various Cat protein fusion reveales that multiple regions in the cat protein are causing the export defect | Bacillus subtilis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Bacillus subtilis | 5737 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + chloramphenicol | - |
Bacillus subtilis | CoA + chloramphenicol 3-acetate | - |
? |  |
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