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Literature summary for 2.3.1.28 extracted from

  • Nolte, G.; Sussmuth, R.
    Purification and characterization of chloramphenicol acetyltransferase from Flavobacterium CB60 (1987), J. Gen. Microbiol., 133, 2115-2122.
    View publication on PubMed
Search for more literature for 2.3.1.28

General Stability

General Stability Organism
loss of activity by freezing and thawing can be prevented by adding 10% glycerol Flavobacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
1,5-Difluoro-2,4-dinitrobenzene
-
 Flavobacterium sp.
5,5'-dithiobis(2-nitrobenzoic acid)
-
 Flavobacterium sp.
NEM
-
 Flavobacterium sp.
PCMB
-
 Flavobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 chloramphenicol
-
 Flavobacterium sp.
0.065
-
 acetyl-CoA
-
 Flavobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26600
-
 3 * 26600, SDS-PAGE Flavobacterium sp.
80000
-
 gel filtration Flavobacterium sp.

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
 CB60
-
Flavobacterium sp. CB60
-
 CB60
-

Purification (Commentary)

Purification (Comment) Organism
-
 Flavobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
367.7
-
-
 Flavobacterium sp.

Storage Stability

Storage Stability Organism
-20 C, stable for several months Flavobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + chloramphenicol
-
 Flavobacterium sp. CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol
-
 Flavobacterium sp. CB60 CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol 1-acetate no activity Flavobacterium sp. CoA + chloramphenicol 1,3-diacetate
-
 ?
acetyl-CoA + chloramphenicol 1-acetate no activity Flavobacterium sp. CB60 CoA + chloramphenicol 1,3-diacetate
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 26600, SDS-PAGE Flavobacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 nearly complete inactivation within 10 min Flavobacterium sp.