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Literature summary for 2.3.1.28 extracted from

  • Cardoso, M.; Schwarz, S.
    Characterization of the chloramphenicol acetyltransferase variants encoded by the plasmids pSCS6 and pSCS7 from Staphylococcus aureus (1992), J. Gen. Microbiol., 138, 275-281.
    View publication on PubMed
Search for more literature for 2.3.1.28

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
 chloramphenicol enzyme from plasmid pSCS6 Staphylococcus aureus
0.0027
-
 chloramphenicol enzyme from plasmid pSCS7 Staphylococcus aureus
0.056
-
 acetyl-CoA enzyme from plasmid pSCS7 Staphylococcus aureus
0.059
-
 acetyl-CoA enzyme from plasmid pSCS6 Staphylococcus aureus

Metals/Ions

Metals/Ions Comment Organism Structure
HgCl2 at 0.1 M and 0.3 mM, no influence on the activity of the pSCS6-encoded enzyme. The activity of the pSCS7-encoded enzyme decreases to 10-15%. At 1.0 mM HgCl2, the pSCS6-encoded enzyme retains 50% of ist original activity, complete inactivation of the enzyme from pSCS7-encoded enzyme Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 3 * 23000, SDS-PAGE Staphylococcus aureus
70000
-
 gel filtration Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + chloramphenicol Staphylococcus aureus inducible enzyme CoA + chloramphenicol 3-acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
 encoded by plasmids pSCS6 or pSCS7
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + chloramphenicol
-
 Staphylococcus aureus CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol inducible enzyme Staphylococcus aureus CoA + chloramphenicol 3-acetate
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 23000, SDS-PAGE Staphylococcus aureus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 15 min, 40% loss of activity of enzyme variant from plasmid pSCS6, 45% loss of activity of enzyme variant pSCS7 Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Staphylococcus aureus