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Literature summary for 2.3.1.28 extracted from
- Characterization of chloramphenicol acetyltransferase from chloramphenicol-resistant Staphylococcus aureus (1968), J. Bacteriol., 95, 28-36.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0027 | - |
chloramphenicol | - |
Staphylococcus aureus |  |
| 0.0037 | - |
D-threo-1-p-nitrophenyl-2-bromoacetamido-1,3-propanediol | - |
Escherichia coli | |
| 0.0061 | - |
chloramphenicol | - |
Escherichia coli | |
| 0.021 | - |
D-threo-1-phenyl-2-dichloroacetamido-1,3-propanediol | - |
Escherichia coli | |
| 0.022 | - |
D-threo-1-p-nitrophenyl-2-bromoacetamido-1,3-propanediol | - |
Staphylococcus aureus | |
| 0.068 | - |
D-threo-1-phenyl-2-dichloroacetamido-1,3-propanediol | - |
Staphylococcus aureus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | - |
sucrose density gradient centrifugation | Escherichia coli |
| 78000 | - |
sucrose density gradient centrifugation | Staphylococcus aureus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + chloramphenicol | Staphylococcus aureus | inducible enzyme | CoA + chloramphenicol 3-acetate | - |
? | |
| acetyl-CoA + chloramphenicol | Escherichia coli | inactivates chloramphenicol | CoA + chloramphenicol 3-acetate | - |
? | |
| acetyl-CoA + chloramphenicol | Staphylococcus aureus | inactivates chloramphenicol | CoA + chloramphenicol 3-acetate | - |
? | |
| acetyl-CoA + chloramphenicol | Staphylococcus aureus C22.1 | inducible enzyme | CoA + chloramphenicol 3-acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Staphylococcus aureus | - |
- |
- |
| Staphylococcus aureus C22.1 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 acetyl-CoA + chloramphenicol | - |
Staphylococcus aureus | 2 CoA + chloramphenicol 1,3-diacetate | - |
? | |
| 2 acetyl-CoA + chloramphenicol | - |
Staphylococcus aureus C22.1 | 2 CoA + chloramphenicol 1,3-diacetate | - |
? | |
| acetyl-CoA + chloramphenicol | - |
Escherichia coli | CoA + chloramphenicol 3-acetate | - |
? | |
| acetyl-CoA + chloramphenicol | acetylates only the biologically active D-threo stereoisomer | Staphylococcus aureus | CoA + chloramphenicol 3-acetate | + chloramphenicol 1,3-diacetate | ? | |
| acetyl-CoA + chloramphenicol | inducible enzyme | Staphylococcus aureus | CoA + chloramphenicol 3-acetate | - |
? | |
| acetyl-CoA + chloramphenicol | inactivates chloramphenicol | Escherichia coli | CoA + chloramphenicol 3-acetate | - |
? | |
| acetyl-CoA + chloramphenicol | inactivates chloramphenicol | Staphylococcus aureus | CoA + chloramphenicol 3-acetate | - |
? | |
| acetyl-CoA + chloramphenicol | acetylates only the biologically active D-threo stereoisomer | Staphylococcus aureus C22.1 | CoA + chloramphenicol 3-acetate | + chloramphenicol 1,3-diacetate | ? | |
| acetyl-CoA + chloramphenicol | inducible enzyme | Staphylococcus aureus C22.1 | CoA + chloramphenicol 3-acetate | - |
? | |
| acetyl-CoA + D-threo-1-p-nitrophenyl-2-bromoacetamido-1,3-propanediol | - |
Staphylococcus aureus | CoA + ? | - |
? | |
| acetyl-CoA + D-threo-1-p-nitrophenyl-2-bromoacetamido-1,3-propanediol | - |
Escherichia coli | CoA + ? | - |
? | |
| acetyl-CoA + D-threo-1-p-nitrophenyl-2-bromoacetamido-1,3-propanediol | - |
Staphylococcus aureus C22.1 | CoA + ? | - |
? | |
| acetyl-CoA + D-threo-1-phenyl-2-dichloroacetamido-1,3-propanediol | - |
Staphylococcus aureus | CoA + ? | - |
? | |
| acetyl-CoA + D-threo-1-phenyl-2-dichloroacetamido-1,3-propanediol | - |
Escherichia coli | CoA + ? | - |
? | |
| acetyl-CoA + D-threo-1-phenyl-2-dichloroacetamido-1,3-propanediol | - |
Staphylococcus aureus C22.1 | CoA + ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
rapid inactivation | Escherichia coli |
| 75 | - |
remarkably resistant | Staphylococcus aureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Escherichia coli |
| 7.8 | - |
- |
Staphylococcus aureus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.8 | pH 6.0: about 55% of maximal activity, pH 8.8: about 85% of maximal activity | Staphylococcus aureus |
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