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Literature summary for 2.3.1.275 extracted from

  • Lu, Y.; Zhang, F.; Grimes, K.; Lee, R.; Rock, C.
    Topology and active site of PlsY The bacterial acylphosphate glycerol-3-phosphate acyltransferase (2007), J. Biol. Chem., 282, 11339-11346 .
    View publication on PubMed
Search for more literature for 2.3.1.275

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptococcus pneumoniae

Protein Variants

Protein Variants Comment Organism
A105C mutant enzyme shows 89.6% of the activity compared to wild-type enzyme Streptococcus pneumoniae
G102A mutant enzyme shows 9.5% of the activity compared to wild-type enzyme Streptococcus pneumoniae
G103A mutant enzyme shows 1.2% of the activity compared to wild-type enzyme Streptococcus pneumoniae
H185A mutant enzyme shows 0.6% of the activity compared to wild-type enzyme Streptococcus pneumoniae
K101A mutant enzyme shows 96.7% of the activity compared to wild-type enzyme Streptococcus pneumoniae
K104A mutant enzyme shows less than 0.05% of the activity compared to wild-type enzyme Streptococcus pneumoniae
K104S mutant enzyme shows less than 0.05% of the activity compared to wild-type enzyme Streptococcus pneumoniae
K186A mutant enzyme shows 53.1% of the activity compared to wild-type enzyme Streptococcus pneumoniae
N188A mutant enzyme shows less than 0.05% of the activity compared to wild-type enzyme Streptococcus pneumoniae
N38A mutant enzyme shows 58.8% of the activity compared to wild-type enzyme Streptococcus pneumoniae
N43A mutant enzyme shows 5.1% of the activity compared to wild-type enzyme Streptococcus pneumoniae
R46A mutant enzyme shows less than 0.05% of the activity compared to wild-type enzyme Streptococcus pneumoniae
R46S mutant enzyme shows less than 0.05% of the activity compared to wild-type enzyme Streptococcus pneumoniae
S36A mutant enzyme shows less than 0.05% of the activity compared to wild-type enzyme Streptococcus pneumoniae
S36C mutant enzyme shows less than 0.05% of the activity compared to wild-type enzyme Streptococcus pneumoniae
T39C mutant enzyme shows 56.0% of the activity compared to wild-type enzyme Streptococcus pneumoniae
T42A mutant enzyme shows 122.7% of the activity compared to wild-type enzyme Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
1-hexadecanoyl-sn-glycerol 3-phosphate product inhibitor Streptococcus pneumoniae
hexadecanoyl-CoA noncompetitive inhibitor Streptococcus pneumoniae
additional information addition of 1 mM EDTA to the reaction does not affect its activity Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 hexadecanoyl phosphate Km-value is determined using purified membranes from Escherichia coli expressing Streptococcus pneumoniae, pH 7.4, 37°C Streptococcus pneumoniae
0.1
-
 sn-glycerol 3-phosphate Km-value is determined using purified membranes from Escherichia coli expressing Streptococcus pneumoniae, pH 7.4, 37°C Streptococcus pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral membrane protein. PlsY has five membrane-spanning segments with the amino terminus and two short loops located on the external face of the membrane. Each of the three larger cytoplasmic domains contains a highly conserved sequence motif. Site-directed mutagenesis reveals that each conserved domain was critical for PlsY catalysis Streptococcus pneumoniae 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information PlsY-The activity of PlsY does not require divalent or monovalent cations Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae P0A4Q0
-
-
Streptococcus pneumoniae ATCC BAA-255 P0A4Q0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hexadecanoyl phosphate + sn-glycerol 3-phosphate
-
 Streptococcus pneumoniae 1-hexadecanoyl-sn-glycerol 3-phosphate + phosphate
-
 ?
hexadecanoyl phosphate + sn-glycerol 3-phosphate
-
 Streptococcus pneumoniae ATCC BAA-255 1-hexadecanoyl-sn-glycerol 3-phosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
PlsY
-
 Streptococcus pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Streptococcus pneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 the enzyme is active between pH 6.5 and pH 9.5 Streptococcus pneumoniae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.012
-
 pH 7.4, 37°C Streptococcus pneumoniae hexadecanoyl-CoA
0.02
-
 pH 7.4, 37°C Streptococcus pneumoniae 1-hexadecanoyl-sn-glycerol 3-phosphate