Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a His-tagged enzyme | Mycobacterium tuberculosis |
expression in Escherichia coli as a His-tagged enzyme | Mycolicibacterium smegmatis |
expression in Escherichia coli as a His-tagged enzyme | Mycolicibacterium hassiacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | 0.4 mM, about 20% inhibition | Mycolicibacterium hassiacum | |
Co2+ | - |
Mycolicibacterium hassiacum | |
EDTA | - |
Mycobacterium tuberculosis | |
EDTA | 10 mM, about 25% loss of activity | Mycolicibacterium hassiacum | |
EDTA | - |
Mycolicibacterium smegmatis | |
hexanoyl-CoA | 0.12 mM, about 50% inhibition | Mycolicibacterium hassiacum | |
Mg2+ | - |
Mycolicibacterium hassiacum | |
octanoyl-CoA | 0.16 mM, about 60% inhibition | Mycolicibacterium hassiacum | |
octanoyl-CoA | 0.16 mM, about 60% inhibition | Mycolicibacterium smegmatis | |
Zn2+ | - |
Mycolicibacterium hassiacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
octanoyl-CoA | 37°C, pH 8.0, cosubstrate: 20 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate | Mycolicibacterium hassiacum | |
0.01 | - |
hexanoyl-CoA | 37°C, pH 8.0, cosubstrate: 20 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate | Mycolicibacterium hassiacum | |
0.02 | - |
hexanoyl-CoA | 37°C, pH 8.0, cosubstrate: 20 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | Mycolicibacterium hassiacum | |
0.02 | - |
octanoyl-CoA | 37°C, pH 8.0, cosubstrate: 45 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate | Mycolicibacterium smegmatis | |
0.03 | - |
octanoyl-CoA | 37°C, pH 8.0, cosubstrate: 45 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | Mycolicibacterium smegmatis | |
0.06 | - |
octanoyl-CoA | 37°C, pH 8.0, cosubstrate: 20 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | Mycolicibacterium hassiacum | |
6.6 | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate | 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA | Mycolicibacterium hassiacum | |
9.5 | - |
2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA | Mycolicibacterium hassiacum | |
9.8 | - |
2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | 37°C, pH 8.0, cosubstrate: 0.1 mM hexanoyl-CoA | Mycolicibacterium hassiacum | |
19 | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate | 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA | Mycolicibacterium smegmatis | |
20 | - |
2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
111100 | - |
gel filtration, tetramer | Mycobacterium tuberculosis |
125200 | - |
gel filtration, tetramer | Mycolicibacterium hassiacum |
125500 | - |
gel filtration, tetramer | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | Mycobacterium tuberculosis | the enzyme is involved in the biosynthesis of methylglucose lipopolysaccharide | 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA | - |
? | |
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | Mycolicibacterium smegmatis | the enzyme is involved in the biosynthesis of methylglucose lipopolysaccharide | 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA | - |
? | |
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | Mycolicibacterium hassiacum | the enzyme is involved in the biosynthesis of methylglucose lipopolysaccharide | 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P71725 | - |
- |
Mycolicibacterium hassiacum | K5BJH8 | - |
- |
Mycolicibacterium smegmatis | A0R109 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
- |
Mycolicibacterium smegmatis |
- |
Mycolicibacterium hassiacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hexanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | - |
Mycobacterium tuberculosis | 2-O-[6-O-hexanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA | - |
? | |
hexanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | - |
Mycolicibacterium hassiacum | 2-O-[6-O-hexanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA | - |
? | |
octanoyl-CoA + 2-O-(alpha-D-glucopyranosyl)-D-glycerate | lower activity than with 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | Mycolicibacterium smegmatis | ? | - |
? | |
octanoyl-CoA + 2-O-(alpha-D-glucopyranosyl)-D-glycerate | lower activity than with 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | Mycolicibacterium hassiacum | ? | - |
? | |
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | - |
Mycobacterium tuberculosis | 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA | - |
? | |
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | - |
Mycolicibacterium smegmatis | 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA | - |
? | |
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | - |
Mycolicibacterium hassiacum | 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA | - |
? | |
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | the enzyme is involved in the biosynthesis of methylglucose lipopolysaccharide | Mycobacterium tuberculosis | 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA | - |
? | |
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | the enzyme is involved in the biosynthesis of methylglucose lipopolysaccharide | Mycolicibacterium smegmatis | 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA | - |
? | |
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | the enzyme is involved in the biosynthesis of methylglucose lipopolysaccharide | Mycolicibacterium hassiacum | 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 *, SDS-PAGE | Mycobacterium tuberculosis |
tetramer | 4 *, SDS-PAGE | Mycolicibacterium smegmatis |
tetramer | 4 *, SDS-PAGE | Mycolicibacterium hassiacum |
Synonyms | Comment | Organism |
---|---|---|
DGG octanoyltransferase | - |
Mycobacterium tuberculosis |
DGG octanoyltransferase | - |
Mycolicibacterium smegmatis |
DGG octanoyltransferase | - |
Mycolicibacterium hassiacum |
octT | - |
Mycobacterium tuberculosis |
octT | - |
Mycolicibacterium smegmatis |
octT | - |
Mycolicibacterium hassiacum |
Rv2418c | locus name | Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Mycolicibacterium hassiacum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | 30°C: about 40% of maximal activity, 50°C: about 50% of maximal activity | Mycolicibacterium hassiacum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00017 | - |
octanoyl-CoA | 37°C, pH 8.0, cosubstrate: 20 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate | Mycolicibacterium hassiacum | |
0.00017 | - |
hexanoyl-CoA | 37°C, pH 8.0, cosubstrate: 20 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate | Mycolicibacterium hassiacum | |
0.00033 | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate | 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA | Mycolicibacterium smegmatis | |
0.00033 | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate | 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA | Mycolicibacterium hassiacum | |
0.00033 | - |
octanoyl-CoA | 37°C, pH 8.0, cosubstrate: 45 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate | Mycolicibacterium smegmatis | |
0.0005 | - |
2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | 37°C, pH 8.0, cosubstrate: 0.1 mM hexanoyl-CoA | Mycolicibacterium hassiacum | |
0.00067 | - |
hexanoyl-CoA | 37°C, pH 8.0, cosubstrate: 20 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | Mycolicibacterium hassiacum | |
0.0017 | - |
2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA | Mycolicibacterium hassiacum | |
0.002 | - |
octanoyl-CoA | 37°C, pH 8.0, cosubstrate: 20 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | Mycolicibacterium hassiacum | |
0.037 | - |
octanoyl-CoA | 37°C, pH 8.0, cosubstrate: 45 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | Mycolicibacterium smegmatis | |
0.038 | - |
2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA | Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | at 37°C | Mycolicibacterium smegmatis |
7 | 8.5 | at 37°C | Mycolicibacterium hassiacum |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is essential for gowth of Mycobacterium tuberculosis. It is involved in the biosynthesis of methylglucose lipopolysaccharide | Mycobacterium tuberculosis |
metabolism | the enzyme is essential for gowth of Mycobacterium tuberculosis. It is involved in the biosynthesis of methylglucose lipopolysaccharide | Mycolicibacterium smegmatis |
metabolism | the enzyme is essential for gowth of Mycobacterium tuberculosis. It is involved in the biosynthesis of methylglucose lipopolysaccharide | Mycolicibacterium hassiacum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00005 | - |
2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | 37°C, pH 8.0, cosubstrate: 0.1 mM hexanoyl-CoA | Mycolicibacterium hassiacum | |
0.00005 | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate | 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA | Mycolicibacterium hassiacum | |
0.00017 | - |
2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA | Mycolicibacterium hassiacum | |
0.0018 | - |
2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA | Mycolicibacterium smegmatis | |
0.002 | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate | 37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA | Mycolicibacterium smegmatis | |
0.018 | - |
octanoyl-CoA | 37°C, pH 8.0, cosubstrate: 20 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate | Mycolicibacterium hassiacum | |
0.022 | - |
hexanoyl-CoA | 37°C, pH 8.0, cosubstrate: 20 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate | Mycolicibacterium hassiacum | |
0.025 | - |
octanoyl-CoA | 37°C, pH 8.0, cosubstrate: 45 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate | Mycolicibacterium smegmatis | |
0.032 | - |
octanoyl-CoA | 37°C, pH 8.0, cosubstrate: 20 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | Mycolicibacterium hassiacum | |
0.035 | - |
hexanoyl-CoA | 37°C, pH 8.0, cosubstrate: 20 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | Mycolicibacterium hassiacum | |
1.43 | - |
octanoyl-CoA | 37°C, pH 8.0, cosubstrate: 45 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate | Mycolicibacterium smegmatis |