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Literature summary for 2.3.1.271 extracted from

  • Nomura, M.; Takagi, H.
    Role of the yeast, acetyltransferase Mpr1 in oxidative stress Regulation of oxygen reactive species caused by a toxic proline catabolism intermediate (2004), Proc. Natl. Acad. Sci. USA, 101, 12616-12621 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.271

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
 L-azetidine-2-carboxylic acid at pH 8.5 and 30°C Saccharomyces cerevisiae
7.01
-
 DELTA1-Pyrroline-5-carboxylate at pH 7.5 and 30°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae E9P8D2
-
-
Saccharomyces cerevisiae Sigma1278b E9P8D2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + DELTA1-pyrroline-5-carboxylate + H2O
-
 Saccharomyces cerevisiae CoA + N-acetyl-glutamate-gamma-semialdehyde
-
 ?
acetyl-CoA + DELTA1-pyrroline-5-carboxylate + H2O
-
 Saccharomyces cerevisiae Sigma1278b CoA + N-acetyl-glutamate-gamma-semialdehyde
-
 ?
acetyl-CoA + L-azetidine-2-carboxylic acid
-
 Saccharomyces cerevisiae CoA + N-acetyl-L-azetidine-2-carboxylic acid
-
 ?
acetyl-CoA + L-azetidine-2-carboxylic acid
-
 Saccharomyces cerevisiae Sigma1278b CoA + N-acetyl-L-azetidine-2-carboxylic acid
-
 ?
acetyl-CoA + L-glutamate-5-semialdehyde
-
 Saccharomyces cerevisiae CoA + N-acetyl-L-glutamate 5-semialdehyde
-
 ?
acetyl-CoA + L-glutamate-5-semialdehyde
-
 Saccharomyces cerevisiae Sigma1278b CoA + N-acetyl-L-glutamate 5-semialdehyde
-
 ?

Synonyms

Synonyms Comment Organism
MPR1
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.9
-
 L-azetidine-2-carboxylic acid at pH 8.5 and 30°C Saccharomyces cerevisiae
121
-
 DELTA1-Pyrroline-5-carboxylate at pH 7.5 and 30°C Saccharomyces cerevisiae

General Information

General Information Comment Organism
physiological function the enzyme protects yeast cells from DELTA1-pyrroline-5-carboxylate-induced oxidative stress by reducing reactive oxygen species Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
17.2
-
 DELTA1-Pyrroline-5-carboxylate at pH 7.5 and 30°C Saccharomyces cerevisiae
19.8
-
 L-azetidine-2-carboxylic acid at pH 8.5 and 30°C Saccharomyces cerevisiae