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Literature summary for 2.3.1.271 extracted from

  • Nasuno, R.; Hirase, S.; Norifune, S.; Watanabe, D.; Takagi, H.
    Structure-based molecular design for thermostabilization of N-acetyltransferase Mpr1 involved in a novel pathway of L-arginine synthesis in yeast (2015), J. Biochem., 159, 271-277 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.271

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
F65L the mutant exhibits slightly lower activity compared to the wild type enzyme Saccharomyces cerevisiae
F65L/N203K the mutant shows lower activity compared to the wild type enzyme Saccharomyces cerevisiae
F65L/N203R the mutant shows lower activity compared to the wild type enzyme Saccharomyces cerevisiae
N203 E the mutant shows about wild type activity Saccharomyces cerevisiae
N203D the mutant shows about wild type activity Saccharomyces cerevisiae
N203K the mutant exhibits higher activity and 2.4fold longer activity half-live at 50°C than the wild type enzyme Saccharomyces cerevisiae
N203R the mutant with about wild type activity exhibits 2.2fold longer activity half-live at 50°C than the wild type enzyme Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-DELTA1-pyrroline 5-carboxylate + H2O Saccharomyces cerevisiae
-
 CoA + N-acetyl-glutamate-gamma-semialdehyde
-
 ?
acetyl-CoA + L-glutamate 5-semialdehyde Saccharomyces cerevisiae
-
 CoA + N-acetyl-L-glutamate 5-semialdehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae E9P8D2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose 6 column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-azetidine-2-carboxylate
-
 Saccharomyces cerevisiae CoA + N-acetyl-L-azetidine-2-carboxylate
-
 ?
acetyl-CoA + L-DELTA1-pyrroline 5-carboxylate + H2O
-
 Saccharomyces cerevisiae CoA + N-acetyl-glutamate-gamma-semialdehyde
-
 ?
acetyl-CoA + L-glutamate 5-semialdehyde
-
 Saccharomyces cerevisiae CoA + N-acetyl-L-glutamate 5-semialdehyde
-
 ?

Synonyms

Synonyms Comment Organism
MPR1
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the wild type enzyme has a half-life of 11.4 min at 50°C Saccharomyces cerevisiae