Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae strains S288C and CKY8 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
F65G | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Saccharomyces cerevisiae |
F65L | the mutant shows higher catalytic efficiency compared to the wild type enzyme | Saccharomyces cerevisiae |
F65L/L117V | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Saccharomyces cerevisiae |
F65L/L117V | the mutant shows higher azetidine-2-carboxylate resistance compared to the wild type | Saccharomyces cerevisiae |
K63P | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Saccharomyces cerevisiae |
K63R | the mutant shows higher azetidine-2-carboxylate resistance compared to the wild type | Saccharomyces cerevisiae |
K63R/F65L | the mutant shows higher catalytic efficiency compared to the wild type enzyme | Saccharomyces cerevisiae |
K63R/F65L/L117V | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Saccharomyces cerevisiae |
L117V | the mutant shows higher catalytic efficiency compared to the wild type enzyme | Saccharomyces cerevisiae |
LK63R/L117V | the mutant shows higher catalytic efficiency compared to the wild type enzyme | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0049 | - |
acetyl-CoA | mutant enzyme K63R/F65L/L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
0.0076 | - |
acetyl-CoA | mutant enzyme L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
0.008 | - |
acetyl-CoA | mutant enzyme K63R, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
0.01 | - |
acetyl-CoA | mutant enzyme F65L, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
0.011 | - |
acetyl-CoA | mutant enzyme LK63R/L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
0.013 | - |
acetyl-CoA | wild type enzyme, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
0.013 | - |
acetyl-CoA | mutant enzyme F65L/L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
0.019 | - |
acetyl-CoA | mutant enzyme K63P, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
0.019 | - |
acetyl-CoA | mutant enzyme K63R/F65L, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
0.14 | - |
acetyl-CoA | mutant enzyme F65G, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
0.7 | - |
azetidine-2-carboxylate | mutant enzyme F65L, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
1.2 | - |
azetidine-2-carboxylate | mutant enzyme K63R, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
1.2 | - |
azetidine-2-carboxylate | mutant enzyme K63R/F65L/L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
1.4 | - |
azetidine-2-carboxylate | mutant enzyme L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
1.6 | - |
azetidine-2-carboxylate | wild type enzyme, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
1.6 | - |
azetidine-2-carboxylate | mutant enzyme K63P, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
1.7 | - |
azetidine-2-carboxylate | mutant enzyme LK63R/L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
1.8 | - |
azetidine-2-carboxylate | mutant enzyme F65L/L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
2.2 | - |
azetidine-2-carboxylate | mutant enzyme K63R/F65L, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
3.3 | - |
azetidine-2-carboxylate | mutant enzyme F65G, at pH 8.5 and 30°C | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-glutamate-5-semialdehyde | Saccharomyces cerevisiae | - |
CoA + N-acetyl-L-glutamate 5-semialdehyde | - |
? | |
acetyl-CoA + L-glutamate-5-semialdehyde | Saccharomyces cerevisiae Sigma1278b | - |
CoA + N-acetyl-L-glutamate 5-semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | E9P8D2 | - |
- |
Saccharomyces cerevisiae Sigma1278b | E9P8D2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + azetidine-2-carboxylate | - |
Saccharomyces cerevisiae | CoA + N-acetyl-azetidine-2-carboxylate | - |
? | |
acetyl-CoA + azetidine-2-carboxylate | - |
Saccharomyces cerevisiae Sigma1278b | CoA + N-acetyl-azetidine-2-carboxylate | - |
? | |
acetyl-CoA + L-glutamate-5-semialdehyde | - |
Saccharomyces cerevisiae | CoA + N-acetyl-L-glutamate 5-semialdehyde | - |
? | |
acetyl-CoA + L-glutamate-5-semialdehyde | - |
Saccharomyces cerevisiae Sigma1278b | CoA + N-acetyl-L-glutamate 5-semialdehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MPR1 | - |
Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the enzyme has a half-life of 8.2 min at 50°C | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 6 | azetidine-2-carboxylate | wild type enzyme, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
9.1 | - |
acetyl-CoA | mutant enzyme K63R/F65L/L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
11 | - |
azetidine-2-carboxylate | mutant enzyme K63R/F65L/L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
15 | - |
acetyl-CoA | mutant enzyme K63P, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
16 | - |
azetidine-2-carboxylate | mutant enzyme F65G, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
17 | - |
acetyl-CoA | mutant enzyme F65G, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
19 | - |
azetidine-2-carboxylate | mutant enzyme K63P, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
27 | - |
acetyl-CoA | mutant enzyme K63R, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
31 | - |
acetyl-CoA | wild type enzyme, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
31 | - |
acetyl-CoA | mutant enzyme F65L, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
31 | - |
azetidine-2-carboxylate | mutant enzyme F65L, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
31 | - |
acetyl-CoA | mutant enzyme F65L/L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
31 | - |
azetidine-2-carboxylate | mutant enzyme K63R, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
38 | - |
azetidine-2-carboxylate | mutant enzyme F65L/L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
38 | - |
acetyl-CoA | mutant enzyme L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
38 | - |
acetyl-CoA | mutant enzyme LK63R/L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
42 | - |
acetyl-CoA | mutant enzyme K63R/F65L, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
46 | - |
azetidine-2-carboxylate | mutant enzyme LK63R/L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
48 | - |
azetidine-2-carboxylate | mutant enzyme L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
51 | - |
azetidine-2-carboxylate | mutant enzyme K63R/F65L, at pH 8.5 and 30°C | Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00012 | - |
acetyl-CoA | mutant enzyme F65G, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
0.0008 | - |
acetyl-CoA | mutant enzyme K63P, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
0.0022 | - |
acetyl-CoA | mutant enzyme K63R/F65L, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
0.0023 | - |
acetyl-CoA | mutant enzyme F65L/L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
0.0023 | - |
acetyl-CoA | mutant enzyme K63R/F65L/L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
0.0025 | - |
acetyl-CoA | wild type enzyme, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
0.0029 | - |
acetyl-CoA | mutant enzyme F65L, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
0.0033 | - |
acetyl-CoA | mutant enzyme K63R, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
0.0037 | - |
acetyl-CoA | mutant enzyme LK63R/L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
0.005 | - |
acetyl-CoA | mutant enzyme L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
4.7 | - |
azetidine-2-carboxylate | mutant enzyme F65G, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
9.5 | - |
azetidine-2-carboxylate | mutant enzyme K63R/F65L/L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
12 | - |
azetidine-2-carboxylate | mutant enzyme K63P, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
21 | - |
azetidine-2-carboxylate | mutant enzyme F65L/L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
22 | - |
azetidine-2-carboxylate | wild type enzyme, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
23 | - |
azetidine-2-carboxylate | mutant enzyme K63R/F65L, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
25 | - |
azetidine-2-carboxylate | mutant enzyme K63R, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
27 | - |
azetidine-2-carboxylate | mutant enzyme LK63R/L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
33 | - |
azetidine-2-carboxylate | mutant enzyme L117V, at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
46 | - |
azetidine-2-carboxylate | mutant enzyme F65L, at pH 8.5 and 30°C | Saccharomyces cerevisiae |