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Literature summary for 2.3.1.271 extracted from

  • Iinoya, K.; Kotani, T.; Sasano, Y.; Takagi, H.
    Engineering of the yeast antioxidant enzyme Mpr1 for enhanced activity and stability (2009), Biotechnol. Bioeng., 103, 341-352 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strains S288C and CKY8 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
F65G the mutant shows reduced catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
F65L the mutant shows higher catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
F65L/L117V the mutant shows reduced catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
F65L/L117V the mutant shows higher azetidine-2-carboxylate resistance compared to the wild type Saccharomyces cerevisiae
K63P the mutant shows reduced catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
K63R the mutant shows higher azetidine-2-carboxylate resistance compared to the wild type Saccharomyces cerevisiae
K63R/F65L the mutant shows higher catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
K63R/F65L/L117V the mutant shows reduced catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
L117V the mutant shows higher catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
LK63R/L117V the mutant shows higher catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0049
-
acetyl-CoA mutant enzyme K63R/F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.0076
-
acetyl-CoA mutant enzyme L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.008
-
acetyl-CoA mutant enzyme K63R, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.01
-
acetyl-CoA mutant enzyme F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.011
-
acetyl-CoA mutant enzyme LK63R/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.013
-
acetyl-CoA wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.013
-
acetyl-CoA mutant enzyme F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.019
-
acetyl-CoA mutant enzyme K63P, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.019
-
acetyl-CoA mutant enzyme K63R/F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.14
-
acetyl-CoA mutant enzyme F65G, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.7
-
azetidine-2-carboxylate mutant enzyme F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
1.2
-
azetidine-2-carboxylate mutant enzyme K63R, at pH 8.5 and 30°C Saccharomyces cerevisiae
1.2
-
azetidine-2-carboxylate mutant enzyme K63R/F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
1.4
-
azetidine-2-carboxylate mutant enzyme L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
1.6
-
azetidine-2-carboxylate wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
1.6
-
azetidine-2-carboxylate mutant enzyme K63P, at pH 8.5 and 30°C Saccharomyces cerevisiae
1.7
-
azetidine-2-carboxylate mutant enzyme LK63R/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
1.8
-
azetidine-2-carboxylate mutant enzyme F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.2
-
azetidine-2-carboxylate mutant enzyme K63R/F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
3.3
-
azetidine-2-carboxylate mutant enzyme F65G, at pH 8.5 and 30°C Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-glutamate-5-semialdehyde Saccharomyces cerevisiae
-
CoA + N-acetyl-L-glutamate 5-semialdehyde
-
?
acetyl-CoA + L-glutamate-5-semialdehyde Saccharomyces cerevisiae Sigma1278b
-
CoA + N-acetyl-L-glutamate 5-semialdehyde
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae E9P8D2
-
-
Saccharomyces cerevisiae Sigma1278b E9P8D2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + azetidine-2-carboxylate
-
Saccharomyces cerevisiae CoA + N-acetyl-azetidine-2-carboxylate
-
?
acetyl-CoA + azetidine-2-carboxylate
-
Saccharomyces cerevisiae Sigma1278b CoA + N-acetyl-azetidine-2-carboxylate
-
?
acetyl-CoA + L-glutamate-5-semialdehyde
-
Saccharomyces cerevisiae CoA + N-acetyl-L-glutamate 5-semialdehyde
-
?
acetyl-CoA + L-glutamate-5-semialdehyde
-
Saccharomyces cerevisiae Sigma1278b CoA + N-acetyl-L-glutamate 5-semialdehyde
-
?

Synonyms

Synonyms Comment Organism
MPR1
-
Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
the enzyme has a half-life of 8.2 min at 50°C Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3 6 azetidine-2-carboxylate wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
9.1
-
acetyl-CoA mutant enzyme K63R/F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
11
-
azetidine-2-carboxylate mutant enzyme K63R/F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
15
-
acetyl-CoA mutant enzyme K63P, at pH 8.5 and 30°C Saccharomyces cerevisiae
16
-
azetidine-2-carboxylate mutant enzyme F65G, at pH 8.5 and 30°C Saccharomyces cerevisiae
17
-
acetyl-CoA mutant enzyme F65G, at pH 8.5 and 30°C Saccharomyces cerevisiae
19
-
azetidine-2-carboxylate mutant enzyme K63P, at pH 8.5 and 30°C Saccharomyces cerevisiae
27
-
acetyl-CoA mutant enzyme K63R, at pH 8.5 and 30°C Saccharomyces cerevisiae
31
-
acetyl-CoA wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
31
-
acetyl-CoA mutant enzyme F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
31
-
azetidine-2-carboxylate mutant enzyme F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
31
-
acetyl-CoA mutant enzyme F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
31
-
azetidine-2-carboxylate mutant enzyme K63R, at pH 8.5 and 30°C Saccharomyces cerevisiae
38
-
azetidine-2-carboxylate mutant enzyme F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
38
-
acetyl-CoA mutant enzyme L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
38
-
acetyl-CoA mutant enzyme LK63R/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
42
-
acetyl-CoA mutant enzyme K63R/F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
46
-
azetidine-2-carboxylate mutant enzyme LK63R/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
48
-
azetidine-2-carboxylate mutant enzyme L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
51
-
azetidine-2-carboxylate mutant enzyme K63R/F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00012
-
acetyl-CoA mutant enzyme F65G, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.0008
-
acetyl-CoA mutant enzyme K63P, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.0022
-
acetyl-CoA mutant enzyme K63R/F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.0023
-
acetyl-CoA mutant enzyme F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.0023
-
acetyl-CoA mutant enzyme K63R/F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.0025
-
acetyl-CoA wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.0029
-
acetyl-CoA mutant enzyme F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.0033
-
acetyl-CoA mutant enzyme K63R, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.0037
-
acetyl-CoA mutant enzyme LK63R/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.005
-
acetyl-CoA mutant enzyme L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
4.7
-
azetidine-2-carboxylate mutant enzyme F65G, at pH 8.5 and 30°C Saccharomyces cerevisiae
9.5
-
azetidine-2-carboxylate mutant enzyme K63R/F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
12
-
azetidine-2-carboxylate mutant enzyme K63P, at pH 8.5 and 30°C Saccharomyces cerevisiae
21
-
azetidine-2-carboxylate mutant enzyme F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
22
-
azetidine-2-carboxylate wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
23
-
azetidine-2-carboxylate mutant enzyme K63R/F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
25
-
azetidine-2-carboxylate mutant enzyme K63R, at pH 8.5 and 30°C Saccharomyces cerevisiae
27
-
azetidine-2-carboxylate mutant enzyme LK63R/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
33
-
azetidine-2-carboxylate mutant enzyme L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
46
-
azetidine-2-carboxylate mutant enzyme F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae