Literature summary for 2.3.1.265 extracted from

  • Svetlikova, Z.; Barath, P.; Jackson, M.; Kordulakova, J.; Mikusova, K.
    Purification and characterization of the acyltransferase involved in biosynthesis of the major mycobacterial cell envelope glycolipid-monoacylated phosphatidylinositol dimannoside (2014), Protein Expr. Purif., 100, 33-39 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS, Rosetta-gami (DE3)pLysS, and C41(DE3)cells Mycolicibacterium smegmatis

Localization

Localization Comment Organism GeneOntology No. Textmining
cell envelope
-
Mycolicibacterium smegmatis
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-
membrane
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Mycolicibacterium smegmatis
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-
cytosol
-
Mycolicibacterium smegmatis
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-

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis A0QWG5
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Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 A0QWG5
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-

Purification (Commentary)

Purification (Comment) Organism
TALON Co2+ affinity resin column chromatography, and Superdex 200 gel filtration Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + 2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol preferred substrate Mycolicibacterium smegmatis CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
?
palmitoyl-CoA + 2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol preferred substrate Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
?
palmitoyl-CoA + 2-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
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Mycolicibacterium smegmatis CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
-
?
palmitoyl-CoA + 2-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
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Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
-
?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Mycolicibacterium smegmatis

Synonyms

Synonyms Comment Organism
MSMEG 2934 protein
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Mycolicibacterium smegmatis