Literature summary for 2.3.1.265 extracted from

Svetlikova, Z.; Barath, P.; Jackson, M.; Kordulakova, J.; Mikusova, K.
Purification and characterization of the acyltransferase involved in biosynthesis of the major mycobacterial cell envelope glycolipid-monoacylated phosphatidylinositol dimannoside (2014), Protein Expr. Purif., 100, 33-39 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)pLysS, Rosetta-gami (DE3)pLysS, and C41(DE3)cells	Mycolicibacterium smegmatis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell envelope	-	Mycolicibacterium smegmatis	30313	-
cytosol	-	Mycolicibacterium smegmatis	5829	-
membrane	-	Mycolicibacterium smegmatis	16020	-

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium smegmatis	A0QWG5	-	-
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084	A0QWG5	-	-

Purification (Commentary)

Purification (Comment)	Organism
TALON Co2+ affinity resin column chromatography, and Superdex 200 gel filtration	Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
palmitoyl-CoA + 2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol	preferred substrate	Mycolicibacterium smegmatis	CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol	-	?
palmitoyl-CoA + 2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol	preferred substrate	Mycolicibacterium smegmatis mc(2)155 / ATCC 700084	CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol	-	?
palmitoyl-CoA + 2-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol	-	Mycolicibacterium smegmatis	CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol	-	?
palmitoyl-CoA + 2-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol	-	Mycolicibacterium smegmatis mc(2)155 / ATCC 700084	CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol	-	?

Subunits

Subunits	Comment	Organism
?	x * 34000, SDS-PAGE	Mycolicibacterium smegmatis

Synonyms

Synonyms	Comment	Organism
MSMEG 2934 protein	-	Mycolicibacterium smegmatis

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Release 2023.1 (January 2023)