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Literature summary for 2.3.1.263 extracted from

  • Jeng, I.M.; Somack, R.; Barker, H.A.
    Ornithine degradation in Clostridium sticklandii: pyridoxal phosphate and coenzyme A dependent thiolytic cleavage of 2-amino-4-ketopentanoate to alanine and acetyl-CoA (1974), Biochemistry, 13, 2989-2903.
    View publication on PubMed
Search for more literature for 2.3.1.263

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzyme is completely inactivated by dialysis against 100 mM cysteine and can be reactivated by addition of pyridoxal 5'-phosphate Acetoanaerobium sticklandii
N-ethylmaleimide
-
 Acetoanaerobium sticklandii
PCMB
-
 Acetoanaerobium sticklandii
pyridoxal
-
 Acetoanaerobium sticklandii
pyridoxamine 5'-phosphate
-
 Acetoanaerobium sticklandii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00025
-
 CoA pH 8.0, 26°C Acetoanaerobium sticklandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + 2-amino-4-ketopentanoate Clostridium subterminale catalyzes a step in the ornithine fermentation pathway acetyl-CoA + D-alanine
-
 r
CoA + 2-amino-4-ketopentanoate Clostridium subterminale SB4 catalyzes a step in the ornithine fermentation pathway acetyl-CoA + D-alanine
-
 r
CoA + 2-amino-4-oxopentanoate + CoA Acetoanaerobium sticklandii catalyzes a step in the ornithine fermentation pathway acetyl-CoA + D-alanine
-
 ?

Organism

Organism UniProt Comment Textmining
Acetoanaerobium sticklandii
-
-
-
Clostridium subterminale
-
 specific 2-amino-4-ketopentanoate thiolase activity is about 10% of the activity in extracts of Clostridium sticklandii
-
Clostridium subterminale SB4
-
 specific 2-amino-4-ketopentanoate thiolase activity is about 10% of the activity in extracts of Clostridium sticklandii
-

Purification (Commentary)

Purification (Comment) Organism
partial Acetoanaerobium sticklandii

Storage Stability

Storage Stability Organism
-20°C, thiolase activity in crude extract remains constant for at least 10 days Acetoanaerobium sticklandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + 2-amino-4-ketopentanoate catalyzes a step in the ornithine fermentation pathway Clostridium subterminale acetyl-CoA + D-alanine
-
 r
CoA + 2-amino-4-ketopentanoate catalyzes a step in the ornithine fermentation pathway Clostridium subterminale SB4 acetyl-CoA + D-alanine
-
 r
CoA + 2-amino-4-oxopentanoate
-
 Acetoanaerobium sticklandii acetyl-CoA + D-alanine
-
 ?
CoA + 2-amino-4-oxopentanoate
-
 Clostridium subterminale acetyl-CoA + D-alanine
-
 ?
CoA + 2-amino-4-oxopentanoate
-
 Clostridium subterminale SB4 acetyl-CoA + D-alanine
-
 ?
CoA + 2-amino-4-oxopentanoate + CoA catalyzes a step in the ornithine fermentation pathway Acetoanaerobium sticklandii acetyl-CoA + D-alanine
-
 ?

Synonyms

Synonyms Comment Organism
AKP-thiolase
-
 Acetoanaerobium sticklandii
AKP-thiolase
-
 Clostridium subterminale

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
24
-
 thiolase activity in crude extract remains constant for at least 2 days Acetoanaerobium sticklandii

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Km: 0.00025 mM. Strictly pyridoxal 5'-phosphate-dependent enzyme. The enzyme is completely inactivated by dialysis against 100 mM cysteine and can be reactivated by addition of pyridoxal 5'-phosphate. Other pyridoxal phosphate analogs and alpha-keto acids cannot replace pyridoxal 5'-phosphate Acetoanaerobium sticklandii