Any feedback?
Literature summary for 2.3.1.26 extracted from
- Identification of putative active site residues of ACAT enzymes (2008), J. Lipid Res., 49, 1770-1781.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the PCDNA3 vector | Chlorocebus aethiops |
| into the PCDNA3 vector | Chlorocebus sabaeus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A1D400N | mutant, evaluation of conserved aspartic acid residues | Chlorocebus aethiops |
| A1D406A | mutant, evaluation of conserved aspartic acid residues | Chlorocebus aethiops |
| A1H386N | mutant, identification of essential histidines required for activity | Chlorocebus aethiops |
| A1H425A | mutant, identification of essential histidines required for activity | Chlorocebus aethiops |
| A1H460A | mutant, identification of essential histidines required for activity | Chlorocebus aethiops |
| A1S128A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus aethiops |
| A1S194A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus aethiops |
| A1S269A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus aethiops |
| A1S410A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus aethiops |
| A1S412A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus aethiops |
| A1S414L | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus aethiops |
| A1S456A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus aethiops |
| A1Y142F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus aethiops |
| A1Y308F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus aethiops |
| A1Y312F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus aethiops |
| A1Y322F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus aethiops |
| A1Y404F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus aethiops |
| A1Y433F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus aethiops |
| A1Y518F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus aethiops |
| A2D378E | mutant, evaluation of conserved aspartic acid residues | Chlorocebus sabaeus |
| A2D384A | mutant, evaluation of conserved aspartic acid residues | Chlorocebus sabaeus |
| A2H364N | mutant, identification of essential histidines required for activity | Chlorocebus sabaeus |
| A2H403A | mutant, identification of essential histidines required for activity | Chlorocebus sabaeus |
| A2H438A | mutant, identification of essential histidines required for activity | Chlorocebus sabaeus |
| A2S109A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus sabaeus |
| A2S176A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus sabaeus |
| A2S249A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus sabaeus |
| A2S388A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus sabaeus |
| A2S390A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus sabaeus |
| A2S392A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus sabaeus |
| A2S434A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus sabaeus |
| A2Y124F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus sabaeus |
| A2Y286F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus sabaeus |
| A2Y290F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus sabaeus |
| A2Y300F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus sabaeus |
| A2Y382F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus sabaeus |
| A2Y411F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus sabaeus |
| A2Y496F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus sabaeus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + cholesterol | Chlorocebus aethiops | - |
CoA + cholesterol ester | - |
? |  |
| acyl-CoA + cholesterol | Chlorocebus sabaeus | - |
CoA + cholesterol ester | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlorocebus aethiops | - |
African green monkey | - |
| Chlorocebus sabaeus | - |
African green monkey | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| microsomes from AC29 cells are prepared | Chlorocebus aethiops |
| microsomes from AC29 cells are prepared | Chlorocebus sabaeus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + cholesterol | - |
Chlorocebus aethiops | CoA + cholesterol ester | - |
? | |
| acyl-CoA + cholesterol | - |
Chlorocebus sabaeus | CoA + cholesterol ester | - |
? | |
| oleoyl-CoA + cholesterol | activity assay | Chlorocebus sabaeus | CoA + cholesteryl oleate | - |
? | |
| oleoyl-CoA + cholesterol | activity assay | Chlorocebus aethiops | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACAT1 | - |
Chlorocebus aethiops |
| ACAT2 | - |
Chlorocebus sabaeus |
| acyl-CoA:cholesterol acyltransferase 1 | - |
Chlorocebus aethiops |
| acyl-CoA:cholesterol acyltransferase 2 | - |
Chlorocebus sabaeus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
microsomal ACAT assay | Chlorocebus aethiops |
| 37 | - |
microsomal ACAT assay | Chlorocebus sabaeus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
