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Literature summary for 2.3.1.26 extracted from

  • Kinnunen, P.M.; DeMichele, A.; Lange, L.G.
    Chemical modification of acyl-CoA:cholesterol O-acyltransferase. 1. Identification of acyl-CoA:cholesterol O-acyltransferase subtypes by differential diethyl pyrocarbonate sensitivity (1988), Biochemistry, 27, 7344-7350.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Acetic anhydride 2 distinct tissue types with difference in reactivity, the inhibitor appears to modify a histidine residue Oryctolagus cuniculus
diethyl dicarbonate 2 distinct tissue types with difference in reactivity, DEP-sensitive subtype typified by aortic ACTAT, DEP-resistant subtype typified by liver ACAT, irreversible inhibition, the inhibitor appear to modify a histidine residue Oryctolagus cuniculus
p-mercuribenzoate 100% inhibition of liver and aortic enzyme at 1 mM Oryctolagus cuniculus
Phenylglyoxal concentration-dependent inhibition of liver enzyme with 65% inhibition at 5 mM Oryctolagus cuniculus
Tetranitromethane 100% inhibition of liver and aortic enzyme at 1 mM Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Oryctolagus cuniculus
-
-

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
a long-chain acyl-CoA + a sterol = CoA + a long-chain 3-hydroxysterol ester mechanism, a histidine is implicated in the catalytic mechanism of the enzyme Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
adipose tissue
-
Oryctolagus cuniculus
-
adrenal gland 1.7fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-
aorta 41fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-
brain 1.6fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-
heart 6fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-
intestine 23fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-
kidney
-
Oryctolagus cuniculus
-
liver 27fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-
lung 3fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-
additional information overview: enzyme activity in normal and cholesterol-fed organs, identification of two distinct tissue types of the enzyme Oryctolagus cuniculus
-
pancreas 25fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-
spleen 7fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-
stomach
-
Oryctolagus cuniculus
-
testis 1.8fold induction of activity after 60 days of cholesterol feeding Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
overview: normal and cholesterol-fed organs Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + cholesterol
-
Oryctolagus cuniculus CoA + cholesterol ester
-
?
oleoyl-CoA + cholesterol
-
Oryctolagus cuniculus CoA + cholesteryl oleate
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.04
-
diethyl dicarbonate aortic enzyme Oryctolagus cuniculus
0.5
-
Acetic anhydride less than, aortic enzyme Oryctolagus cuniculus
1.5
-
diethyl dicarbonate liver enzyme Oryctolagus cuniculus
5
-
Acetic anhydride greater than, liver enzyme Oryctolagus cuniculus