Literature summary for 2.3.1.259 extracted from

Stove, S.; Magin, R.; Foyn, H.; Haug, B.; Marmorstein, R.; Arnesen, T.
Crystal structure of the Golgi-associated human Nalpha-acetyltransferase 60 reveals the molecular determinants for substrate-specific acetylation (2016), Structure, 24, 1044-1056.

Search for more literature for 2.3.1.259

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta (DE3) pLys cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 3% (w/v) tacsimate, 0.1 M bis-Tris (pH 6.5), and 16% polyethylene glycol (PEG) 3350	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D81A	the mutant shows 136.9% catalytic efficiency compared to the wild type enzyme	Homo sapiens
H138A	the mutant is unstable and cannot be purified	Homo sapiens
H138F	the mutant is unstable and cannot be purified	Homo sapiens
I167A	the mutant shows an approximate 2.5 to 4fold reduction in kcat and a small increase in KM value, so the catalytic efficiency of this mutant is approximately 3fold of the wild type enzyme	Homo sapiens
I36A	the mutant shows a 6fold reduction in catalytic efficiency compared to the wild type enzyme	Homo sapiens
I84A	the mutant shows 200.4% catalytic efficiency compared to the wild type enzyme	Homo sapiens
L140A	the mutant has a 4-5fold increase in kcat but at the same time a 5-10fold increase in KM value , so the catalytic efficiency of this mutant is approximately 0.5fold of the wild type enzyme	Homo sapiens
P35A	the mutant shows a 6fold reduction in catalytic efficiency compared to the wild type enzyme	Homo sapiens
P35A/I36A/I167A	inactive	Homo sapiens
Y164A	the mutant shows 141.7% catalytic efficiency compared to the wild type enzyme	Homo sapiens
Y164F	the mutant shows 154.9% catalytic efficiency compared to the wild type enzyme	Homo sapiens
Y165F	the mutant has an approximately 4fold reduction in catalytic efficiency compared to the wild type enzyme	Homo sapiens
Y38A	inactive	Homo sapiens
Y97F	the mutant shows complete loss of catalytic activity	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
CoA-Ac-MAPLDLD-NH2	very potent inhibitor	Homo sapiens
CoA-Ac-MKAVQAD-NH2	very potent inhibitor	Homo sapiens
desulfo-CoA	-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi apparatus	-	Homo sapiens	5794	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + an N-terminal-L-methionyl-[transmembrane protein]	Homo sapiens	-	an N-terminal-Nalpha-acetyl-L-methionyl-[transmembrane protein] + CoA	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9H7X0	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel resin column chromatography and Superdex 200 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + an N-terminal-L-methionyl-[transmembrane protein]	-	Homo sapiens	an N-terminal-Nalpha-acetyl-L-methionyl-[transmembrane protein] + CoA	-	?
acetyl-CoA + MAPL	best substrate	Homo sapiens	CoA + N-acetyl-MAPL	-	?
acetyl-CoA + MKAV	-	Homo sapiens	CoA + N-acetyl-MKAV	-	?
acetyl-CoA + MKQY	-	Homo sapiens	CoA + N-acetyl-MKQY	-	?
acetyl-CoA + MLGP	worst substrate	Homo sapiens	CoA + N-acetyl-MLGP	-	?
acetyl-CoA + MVSM	-	Homo sapiens	CoA + N-acetyl-MVSM	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Homo sapiens

Synonyms

Synonyms	Comment	Organism
Naa60	-	Homo sapiens
Nalpha-acetyltransferase 60	-	Homo sapiens
NatF	-	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.001	-	pH and temperature not specified in the publication	Homo sapiens	CoA-Ac-MAPLDLD-NH2
0.00244	-	pH and temperature not specified in the publication	Homo sapiens	CoA-Ac-MKAVQAD-NH2
0.395	-	pH and temperature not specified in the publication	Homo sapiens	desulfo-CoA

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Release 2023.1 (January 2023)