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Literature summary for 2.3.1.257 extracted from

  • Hole, K.; van Damme, P.; Dalva, M.; Aksnes, H.; Glomnes, N.; Varhaug, J.; Lillehaug, J.; Gevaert, K.; Arnesen, T.
    The human N-alpha-acetyltransferase 40 (hNaa40p/hNatD) is conserved from yeast and N-terminally acetylates histones H2A and H4 (2011), PLoS ONE, 6, e24713.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.257

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star(DE3) cells Homo sapiens
recombinant expression of hNaa40p in Escherichia coli strain BL21 StarTM (DE3), and of hNaa40p-V5 in HEK293 cells. Heterologous ectopically expression of hNaa40p in a Saccharomyces cerevisiae naa40-DELTA strain restores Nt-acetylation of yeast histone H4, but not H2A in vivo Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-
additional information endogenous hNaa40p localizes to both the cytoplasm and the nuclei Homo sapiens
-
-
nucleus
-
 Homo sapiens 5634
-
ribosome
-
 Homo sapiens 5840
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + an N-terminal-L-seryl-[histone H2A] Homo sapiens
-
 an N-terminal-Nalpha-acetyl-L-seryl-[histone H2A] + CoA
-
 ?
acetyl-CoA + an N-terminal-L-seryl-[histone H4] Homo sapiens
-
 an N-terminal-Nalpha-acetyl-L-seryl-[histone H4] + CoA
-
 ?
acetyl-CoA + peptide Homo sapiens hNaa40p preferentially acetylates Ser-Gly-Gly-Gly-Lys- starting peptides CoA + Nalpha-acetylpeptide
-
 ?
additional information Homo sapiens hNaa40p is a NAT selectively acetylating Ser-Gly or Gly-Gly starting peptides. NatD also is defined as the catalytic unit Naa40p (Nat4) which co-translationally Nt-acetylates histones H2A and H4. Recombinant hNaa40p Nt-acetylates the oligopeptides derived from the N-termini of both histones ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q86UY6
-
-
Homo sapiens Q86UY6 NatD
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 75 gel filtration Homo sapiens
recombinant hNaa40p-V5 from HEK293 cells by immunoprecipitation Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
A-431 cell
-
 Homo sapiens
-
HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + an N-terminal-L-seryl-[histone H2A]
-
 Homo sapiens an N-terminal-Nalpha-acetyl-L-seryl-[histone H2A] + CoA
-
 ?
acetyl-CoA + an N-terminal-L-seryl-[histone H4]
-
 Homo sapiens an N-terminal-Nalpha-acetyl-L-seryl-[histone H4] + CoA
-
 ?
acetyl-CoA + histone H2A Saccharomyces cerevisiae histone H2A, 2SGGKGGKAGSAAKASQSR19 N-terminal sequence Homo sapiens CoA + Nalpha-acetyl-histone H2A
-
 ?
acetyl-CoA + histone H4 Saccharomyces cerevisiae histone H4, 2SGRGKGGKGLGKGGAKR18 N-terminal sequence. hNaa40p catalyzes H4 Nalpha-acetylation and not H4 lysine Nepsilon-acetylation, immunoprecipitated hNaa40p specifically Nt-acetylates H4 in vitro Homo sapiens CoA + Nalpha-acetyl-histone H4
-
 ?
acetyl-CoA + peptide
-
 Homo sapiens CoA + Nalpha-acetylpeptide
-
 ?
acetyl-CoA + peptide hNaa40p preferentially acetylates Ser-Gly-Gly-Gly-Lys- starting peptides Homo sapiens CoA + Nalpha-acetylpeptide
-
 ?
additional information hNaa40p is a NAT selectively acetylating Ser-Gly or Gly-Gly starting peptides. NatD also is defined as the catalytic unit Naa40p (Nat4) which co-translationally Nt-acetylates histones H2A and H4. Recombinant hNaa40p Nt-acetylates the oligopeptides derived from the N-termini of both histones Homo sapiens ?
-
 ?
additional information specificity determination using an in vitro proteome-derived peptide library Nt-acetylation assay, in vitro acetylation using synthetic oligopeptides as substrates, overview Homo sapiens ?
-
 ?
additional information the enzyme does not catalyze H4 lysine Nepsilon-acetylation Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
hNaa40p/hNatD
-
 Homo sapiens
N-alpha-acetyltransferase 40
-
 Homo sapiens
Naa40p
-
 Homo sapiens
Naa40p catalytic subunit of NatD, also termed Nat4 Homo sapiens
NAT4
-
 Homo sapiens
NatD
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Homo sapiens