Cloned (Comment) | Organism |
---|---|
gene Rv3420c or rimI, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | I6YG32 | - |
- |
Mycobacterium tuberculosis ATCC 25618 | I6YG32 | - |
- |
Mycobacterium tuberculosis H37Rv | I6YG32 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme RimI from Escherichia coli by nickel affinity and gel filtration | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
600 | - |
about, purified recombinant RimI, NatA substrate N-terminal L-alanyl-[RYFRR], pH 8.0, 25°C | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + N-terminal L-alanyl-[KVNIK] | DP7 peptide (AKVNIK) is a substrate of NatA and is derived from the protein endoded by groES/Rv3418c (Mtb), a neighboring non-ribosomal protein | Mycobacterium tuberculosis | CoA + H+ + N-terminal Nalpha-acetyl-L-alanyl-[KVNIK] | - |
ir | |
acetyl-CoA + N-terminal L-alanyl-[KVNIK] | DP7 peptide (AKVNIK) is a substrate of NatA and is derived from the protein endoded by groES/Rv3418c (Mtb), a neighboring non-ribosomal protein | Mycobacterium tuberculosis H37Rv | CoA + H+ + N-terminal Nalpha-acetyl-L-alanyl-[KVNIK] | - |
ir | |
acetyl-CoA + N-terminal L-alanyl-[KVNIK] | DP7 peptide (AKVNIK) is a substrate of NatA and is derived from the protein endoded by groES/Rv3418c (Mtb), a neighboring non-ribosomal protein | Mycobacterium tuberculosis ATCC 25618 | CoA + H+ + N-terminal Nalpha-acetyl-L-alanyl-[KVNIK] | - |
ir | |
acetyl-CoA + N-terminal L-alanyl-[RYFRR] | DPC peptide (ARYFRR) is a substrate of NatA and is derived from the sequence of S18 RNA protein rpsRS18 of Salmonella typhimurium | Mycobacterium tuberculosis | CoA + H+ + N-terminal Nalpha-acetyl-L-alanyl-[RYFRR] | - |
ir | |
acetyl-CoA + N-terminal L-alanyl-[RYFRR] | DPC peptide (ARYFRR) is a substrate of NatA and is derived from the sequence of S18 RNA protein rpsRS18 of Salmonella typhimurium | Mycobacterium tuberculosis H37Rv | CoA + H+ + N-terminal Nalpha-acetyl-L-alanyl-[RYFRR] | - |
ir | |
acetyl-CoA + N-terminal L-alanyl-[RYFRR] | DPC peptide (ARYFRR) is a substrate of NatA and is derived from the sequence of S18 RNA protein rpsRS18 of Salmonella typhimurium | Mycobacterium tuberculosis ATCC 25618 | CoA + H+ + N-terminal Nalpha-acetyl-L-alanyl-[RYFRR] | - |
ir | |
acetyl-CoA + N-terminal L-seryl-[KLIEY] | DP6 peptide (SKLIEY) is a substrate of NatA and is derived from the protein endoded by tsaE/Rv3422c (Mtb), a neighboring non-ribosomal protein | Mycobacterium tuberculosis | CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[KLIEY] | - |
ir | |
acetyl-CoA + N-terminal L-seryl-[KLIEY] | DP6 peptide (SKLIEY) is a substrate of NatA and is derived from the protein endoded by tsaE/Rv3422c (Mtb), a neighboring non-ribosomal protein | Mycobacterium tuberculosis H37Rv | CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[KLIEY] | - |
ir | |
acetyl-CoA + N-terminal L-seryl-[KLIEY] | DP6 peptide (SKLIEY) is a substrate of NatA and is derived from the protein endoded by tsaE/Rv3422c (Mtb), a neighboring non-ribosomal protein | Mycobacterium tuberculosis ATCC 25618 | CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[KLIEY] | - |
ir | |
acetyl-CoA + N-terminal L-seryl-[RVQIS] | DP4 peptide (SRVQIS) is a substrate of NatA and is derived from the protein endoded by tsaB/Rv3421c (Mtb), a neighboring non-ribosomal protein | Mycobacterium tuberculosis | CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[RVQIS] | - |
ir | |
acetyl-CoA + N-terminal L-seryl-[RVQIS] | DP4 peptide (SRVQIS) is a substrate of NatA and is derived from the protein endoded by tsaB/Rv3421c (Mtb), a neighboring non-ribosomal protein | Mycobacterium tuberculosis H37Rv | CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[RVQIS] | - |
ir | |
acetyl-CoA + N-terminal L-seryl-[RVQIS] | DP4 peptide (SRVQIS) is a substrate of NatA and is derived from the protein endoded by tsaB/Rv3421c (Mtb), a neighboring non-ribosomal protein | Mycobacterium tuberculosis ATCC 25618 | CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[RVQIS] | - |
ir | |
additional information | analysis of substrate preference of RimIMtb: substrate peptide DPC (NatA substrate) is custom synthesized with single residue modifications at its N-terminus to represent substrate specificities of NatE (DP9), NatB (DP10), NatC (DP11), and substrate Leu (DP8) and tested, all the peptides are modified by RimIMtb, substrates and sequences, detailed overview. RimIMtb acetylates N-terminus of ribosomal proteins and of neighboring non-ribosomal proteins. The NatB substrate peptide MERYFRR is a poor substrate for RimI. RimIMtb does acetylate peptides representing N-terminus of GroES, GroEL1, and TsaD proteins, in vitro. Significant specific activity of RimIMtb is observed against peptide representing N-terminus of GroES | Mycobacterium tuberculosis | ? | - |
- |
|
additional information | analysis of substrate preference of RimIMtb: substrate peptide DPC (NatA substrate) is custom synthesized with single residue modifications at its N-terminus to represent substrate specificities of NatE (DP9), NatB (DP10), NatC (DP11), and substrate Leu (DP8) and tested, all the peptides are modified by RimIMtb, substrates and sequences, detailed overview. RimIMtb acetylates N-terminus of ribosomal proteins and of neighboring non-ribosomal proteins. The NatB substrate peptide MERYFRR is a poor substrate for RimI. RimIMtb does acetylate peptides representing N-terminus of GroES, GroEL1, and TsaD proteins, in vitro. Significant specific activity of RimIMtb is observed against peptide representing N-terminus of GroES | Mycobacterium tuberculosis H37Rv | ? | - |
- |
|
additional information | analysis of substrate preference of RimIMtb: substrate peptide DPC (NatA substrate) is custom synthesized with single residue modifications at its N-terminus to represent substrate specificities of NatE (DP9), NatB (DP10), NatC (DP11), and substrate Leu (DP8) and tested, all the peptides are modified by RimIMtb, substrates and sequences, detailed overview. RimIMtb acetylates N-terminus of ribosomal proteins and of neighboring non-ribosomal proteins. The NatB substrate peptide MERYFRR is a poor substrate for RimI. RimIMtb does acetylate peptides representing N-terminus of GroES, GroEL1, and TsaD proteins, in vitro. Significant specific activity of RimIMtb is observed against peptide representing N-terminus of GroES | Mycobacterium tuberculosis ATCC 25618 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
Nalpha-acetyltransferase | - |
Mycobacterium tuberculosis |
NatA | - |
Mycobacterium tuberculosis |
RimI | - |
Mycobacterium tuberculosis |
RimI acetyltransferase | - |
Mycobacterium tuberculosis |
Rv3420c | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
physiological function | Nalpha-acetylation is a naturally occurring irreversible modification of N-termini of proteins catalyzed by Nalpha-acetyltransferases (NATs) | Mycobacterium tuberculosis |