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Literature summary for 2.3.1.255 extracted from

  • Pathak, D.; Bhat, A.; Sapehia, V.; Rai, J.; Rao, A.
    Biochemical evidence for relaxed substrate specificity of Nalpha-acetyltransferase (Rv3420c/rimI) of Mycobacterium tuberculosis (2016), Sci. Rep., 6, 28892 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene Rv3420c or rimI, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis I6YG32
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Mycobacterium tuberculosis ATCC 25618 I6YG32
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Mycobacterium tuberculosis H37Rv I6YG32
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Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged enzyme RimI from Escherichia coli by nickel affinity and gel filtration Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
600
-
about, purified recombinant RimI, NatA substrate N-terminal L-alanyl-[RYFRR], pH 8.0, 25°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + N-terminal L-alanyl-[KVNIK] DP7 peptide (AKVNIK) is a substrate of NatA and is derived from the protein endoded by groES/Rv3418c (Mtb), a neighboring non-ribosomal protein Mycobacterium tuberculosis CoA + H+ + N-terminal Nalpha-acetyl-L-alanyl-[KVNIK]
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ir
acetyl-CoA + N-terminal L-alanyl-[KVNIK] DP7 peptide (AKVNIK) is a substrate of NatA and is derived from the protein endoded by groES/Rv3418c (Mtb), a neighboring non-ribosomal protein Mycobacterium tuberculosis H37Rv CoA + H+ + N-terminal Nalpha-acetyl-L-alanyl-[KVNIK]
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ir
acetyl-CoA + N-terminal L-alanyl-[KVNIK] DP7 peptide (AKVNIK) is a substrate of NatA and is derived from the protein endoded by groES/Rv3418c (Mtb), a neighboring non-ribosomal protein Mycobacterium tuberculosis ATCC 25618 CoA + H+ + N-terminal Nalpha-acetyl-L-alanyl-[KVNIK]
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ir
acetyl-CoA + N-terminal L-alanyl-[RYFRR] DPC peptide (ARYFRR) is a substrate of NatA and is derived from the sequence of S18 RNA protein rpsRS18 of Salmonella typhimurium Mycobacterium tuberculosis CoA + H+ + N-terminal Nalpha-acetyl-L-alanyl-[RYFRR]
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ir
acetyl-CoA + N-terminal L-alanyl-[RYFRR] DPC peptide (ARYFRR) is a substrate of NatA and is derived from the sequence of S18 RNA protein rpsRS18 of Salmonella typhimurium Mycobacterium tuberculosis H37Rv CoA + H+ + N-terminal Nalpha-acetyl-L-alanyl-[RYFRR]
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ir
acetyl-CoA + N-terminal L-alanyl-[RYFRR] DPC peptide (ARYFRR) is a substrate of NatA and is derived from the sequence of S18 RNA protein rpsRS18 of Salmonella typhimurium Mycobacterium tuberculosis ATCC 25618 CoA + H+ + N-terminal Nalpha-acetyl-L-alanyl-[RYFRR]
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ir
acetyl-CoA + N-terminal L-seryl-[KLIEY] DP6 peptide (SKLIEY) is a substrate of NatA and is derived from the protein endoded by tsaE/Rv3422c (Mtb), a neighboring non-ribosomal protein Mycobacterium tuberculosis CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[KLIEY]
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ir
acetyl-CoA + N-terminal L-seryl-[KLIEY] DP6 peptide (SKLIEY) is a substrate of NatA and is derived from the protein endoded by tsaE/Rv3422c (Mtb), a neighboring non-ribosomal protein Mycobacterium tuberculosis H37Rv CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[KLIEY]
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ir
acetyl-CoA + N-terminal L-seryl-[KLIEY] DP6 peptide (SKLIEY) is a substrate of NatA and is derived from the protein endoded by tsaE/Rv3422c (Mtb), a neighboring non-ribosomal protein Mycobacterium tuberculosis ATCC 25618 CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[KLIEY]
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ir
acetyl-CoA + N-terminal L-seryl-[RVQIS] DP4 peptide (SRVQIS) is a substrate of NatA and is derived from the protein endoded by tsaB/Rv3421c (Mtb), a neighboring non-ribosomal protein Mycobacterium tuberculosis CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[RVQIS]
-
ir
acetyl-CoA + N-terminal L-seryl-[RVQIS] DP4 peptide (SRVQIS) is a substrate of NatA and is derived from the protein endoded by tsaB/Rv3421c (Mtb), a neighboring non-ribosomal protein Mycobacterium tuberculosis H37Rv CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[RVQIS]
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ir
acetyl-CoA + N-terminal L-seryl-[RVQIS] DP4 peptide (SRVQIS) is a substrate of NatA and is derived from the protein endoded by tsaB/Rv3421c (Mtb), a neighboring non-ribosomal protein Mycobacterium tuberculosis ATCC 25618 CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[RVQIS]
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ir
additional information analysis of substrate preference of RimIMtb: substrate peptide DPC (NatA substrate) is custom synthesized with single residue modifications at its N-terminus to represent substrate specificities of NatE (DP9), NatB (DP10), NatC (DP11), and substrate Leu (DP8) and tested, all the peptides are modified by RimIMtb, substrates and sequences, detailed overview. RimIMtb acetylates N-terminus of ribosomal proteins and of neighboring non-ribosomal proteins. The NatB substrate peptide MERYFRR is a poor substrate for RimI. RimIMtb does acetylate peptides representing N-terminus of GroES, GroEL1, and TsaD proteins, in vitro. Significant specific activity of RimIMtb is observed against peptide representing N-terminus of GroES Mycobacterium tuberculosis ?
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additional information analysis of substrate preference of RimIMtb: substrate peptide DPC (NatA substrate) is custom synthesized with single residue modifications at its N-terminus to represent substrate specificities of NatE (DP9), NatB (DP10), NatC (DP11), and substrate Leu (DP8) and tested, all the peptides are modified by RimIMtb, substrates and sequences, detailed overview. RimIMtb acetylates N-terminus of ribosomal proteins and of neighboring non-ribosomal proteins. The NatB substrate peptide MERYFRR is a poor substrate for RimI. RimIMtb does acetylate peptides representing N-terminus of GroES, GroEL1, and TsaD proteins, in vitro. Significant specific activity of RimIMtb is observed against peptide representing N-terminus of GroES Mycobacterium tuberculosis H37Rv ?
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additional information analysis of substrate preference of RimIMtb: substrate peptide DPC (NatA substrate) is custom synthesized with single residue modifications at its N-terminus to represent substrate specificities of NatE (DP9), NatB (DP10), NatC (DP11), and substrate Leu (DP8) and tested, all the peptides are modified by RimIMtb, substrates and sequences, detailed overview. RimIMtb acetylates N-terminus of ribosomal proteins and of neighboring non-ribosomal proteins. The NatB substrate peptide MERYFRR is a poor substrate for RimI. RimIMtb does acetylate peptides representing N-terminus of GroES, GroEL1, and TsaD proteins, in vitro. Significant specific activity of RimIMtb is observed against peptide representing N-terminus of GroES Mycobacterium tuberculosis ATCC 25618 ?
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Synonyms

Synonyms Comment Organism
Nalpha-acetyltransferase
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Mycobacterium tuberculosis
NatA
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Mycobacterium tuberculosis
RimI
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Mycobacterium tuberculosis
RimI acetyltransferase
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Mycobacterium tuberculosis
Rv3420c
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Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
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assay at Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
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Mycobacterium tuberculosis

General Information

General Information Comment Organism
physiological function Nalpha-acetylation is a naturally occurring irreversible modification of N-termini of proteins catalyzed by Nalpha-acetyltransferases (NATs) Mycobacterium tuberculosis