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Literature summary for 2.3.1.255 extracted from

  • Liszczak, G.; Goldberg, J.M.; Foyn, H.; Petersson, E.J.; Arnesen, T.; Marmorstein, R.
    Molecular basis for N-terminal acetylation by the heterodimeric NatA complex (2013), Nat. Struct. Mol. Biol., 20, 1098-1105.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.255

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of the 100 kDa holo-NatA complex in the absence and presence of a bisubstrate peptide-CoA conjugate inhibitor, and structure of the uncomplexed Naa10p catalytic subunit. The NatA-Naa15p auxiliary subunit contains 13 TPR motifs and adopts a ring-like topology that wraps around the NatA-Naa10p subunit, an interaction that alters the Naa10p active site for substrate-specific acetylation Schizosaccharomyces pombe

Protein Variants

Protein Variants Comment Organism
E24A mutation in NatA, decrease in kcat, increase in Km value Schizosaccharomyces pombe
E24D mutation in NatA, decrease in kcat, increase in Km value Schizosaccharomyces pombe
E24Q mutation in NatA, decrease in kcat, increase in Km value Schizosaccharomyces pombe
E61A mutation in NatA, decrease in kcat, increase in Km value Schizosaccharomyces pombe
E62A mutation in NatA, decrease in kcat, increase in Km value Schizosaccharomyces pombe
H111A mutation in NatA, decrease in kcat, Km value similar to wild-type Schizosaccharomyces pombe
H20A mutation in NatA, decrease in kcat, increase in Km value Schizosaccharomyces pombe
H72A mutation in NatA, decrease in kcat, increase in Km value Schizosaccharomyces pombe
K29A mutation in NatA, increase in kcat, decrease in Km value Schizosaccharomyces pombe
K29A/Y33A mutation in NatA, decrease in kcat, Km value similar to wild-type Schizosaccharomyces pombe
K59A mutation in NatA, decrease in kcat, increase in Km value Schizosaccharomyces pombe
K59A/E61A mutation in NatA, decrease in kcat, increase in Km value Schizosaccharomyces pombe
K59A/E62A mutation in NatA, decrease in kcat, increase in Km value Schizosaccharomyces pombe
L22A mutation in NatA, decrease in kcat, increase in Km value Schizosaccharomyces pombe
P23A mutation in NatA, decrease in kcat, increase in Km value Schizosaccharomyces pombe
R113A mutation in NatA, strong decrease in kcat, Km value similar to wild-type Schizosaccharomyces pombe
R80A mutation in NatA, decrease in kcat, increase in Km value Schizosaccharomyces pombe
Y139A mutation in NatA, dramaitc loss of activity Schizosaccharomyces pombe
Y26A mutation in NatA, decrease in kcat, increase in Km value Schizosaccharomyces pombe
Y33A mutation in NatA, decrease in kcat, increase in Km value Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
acetonyl-CoA
-
 Schizosaccharomyces pombe
CoA-SASEA CoA covalently linked to substrate peptide fragment Ser1-Ala2-Ser3-Glu4-Ala5 Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 SASEAGVRWGRPVGRRRRP mutant K29A, pH 8.0, 25°C Schizosaccharomyces pombe
0.33
-
 SASEAGVRWGRPVGRRRRP mutant H111A, pH 8.0, 25°C Schizosaccharomyces pombe
0.34
-
 SASEAGVRWGRPVGRRRRP wild-type, pH 8.0, 25°C Schizosaccharomyces pombe
0.34
-
 SASEAGVRWGRPVGRRRRP mutant K29A/Y33A, pH 8.0, 25°C Schizosaccharomyces pombe
0.38
-
 SASEAGVRWGRPVGRRRRP mutant R113A, pH 8.0, 25°C Schizosaccharomyces pombe
0.41
-
 SASEAGVRWGRPVGRRRRP mutant E61A, pH 8.0, 25°C Schizosaccharomyces pombe
0.41
-
 SASEAGVRWGRPVGRRRRP mutant K59A/E62A, pH 8.0, 25°C Schizosaccharomyces pombe
0.44
-
 SASEAGVRWGRPVGRRRRP mutant E24Q, pH 8.0, 25°C Schizosaccharomyces pombe
0.51
-
 SASEAGVRWGRPVGRRRRP mutant K29A, pH 8.0, 25°C Schizosaccharomyces pombe
0.52
-
 SASEAGVRWGRPVGRRRRP mutant K59A/E61A, pH 8.0, 25°C Schizosaccharomyces pombe
0.59
-
 SASEAGVRWGRPVGRRRRP mutant E62A, pH 8.0, 25°C Schizosaccharomyces pombe
0.6
-
 SASEAGVRWGRPVGRRRRP mutant H72A, pH 8.0, 25°C Schizosaccharomyces pombe
0.73
-
 SASEAGVRWGRPVGRRRRP mutant Y26A, pH 8.0, 25°C Schizosaccharomyces pombe
0.74
-
 SASEAGVRWGRPVGRRRRP mutant P23A, pH 8.0, 25°C Schizosaccharomyces pombe
0.75
-
 SASEAGVRWGRPVGRRRRP mutant E24D, pH 8.0, 25°C Schizosaccharomyces pombe
0.76
-
 SASEAGVRWGRPVGRRRRP mutant K59A, pH 8.0, 25°C Schizosaccharomyces pombe
0.77
-
 SASEAGVRWGRPVGRRRRP mutant R80A, pH 8.0, 25°C Schizosaccharomyces pombe
0.92
-
 SASEAGVRWGRPVGRRRRP mutant H20A, pH 8.0, 25°C Schizosaccharomyces pombe
1.85
-
 SASEAGVRWGRPVGRRRRP mutant L22A, pH 8.0, 25°C Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe O74985
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + SASEAGVRWGRPVGRRRRP
-
 Schizosaccharomyces pombe CoA + Nalpha-acetyl-SASEAGVRWGRPVGRRRRP
-
 ?

Synonyms

Synonyms Comment Organism
NAT1
-
 Schizosaccharomyces pombe
NatA
-
 Schizosaccharomyces pombe

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.025
-
 SASEAGVRWGRPVGRRRRP mutant E24Q, pH 8.0, 25°C Schizosaccharomyces pombe
0.047
-
 SASEAGVRWGRPVGRRRRP mutant Y26A, pH 8.0, 25°C Schizosaccharomyces pombe
0.081
-
 SASEAGVRWGRPVGRRRRP mutant L22A, pH 8.0, 25°C Schizosaccharomyces pombe
0.19
-
 SASEAGVRWGRPVGRRRRP mutant R113A, pH 8.0, 25°C Schizosaccharomyces pombe
0.42
-
 SASEAGVRWGRPVGRRRRP mutant E24D, pH 8.0, 25°C Schizosaccharomyces pombe
0.44
-
 SASEAGVRWGRPVGRRRRP mutant Y33A, pH 8.0, 25°C Schizosaccharomyces pombe
0.61
-
 SASEAGVRWGRPVGRRRRP mutant K29A/Y33A, pH 8.0, 25°C Schizosaccharomyces pombe
0.73
-
 SASEAGVRWGRPVGRRRRP mutant K59A/E62A, pH 8.0, 25°C Schizosaccharomyces pombe
0.85
-
 SASEAGVRWGRPVGRRRRP mutant R80A, pH 8.0, 25°C Schizosaccharomyces pombe
1.1
-
 SASEAGVRWGRPVGRRRRP mutant E61A, pH 8.0, 25°C Schizosaccharomyces pombe
1.1
-
 SASEAGVRWGRPVGRRRRP mutant E62A, pH 8.0, 25°C Schizosaccharomyces pombe
1.5
-
 SASEAGVRWGRPVGRRRRP mutant H20A, pH 8.0, 25°C Schizosaccharomyces pombe
1.5
-
 SASEAGVRWGRPVGRRRRP mutant K59A, pH 8.0, 25°C Schizosaccharomyces pombe
1.7
-
 SASEAGVRWGRPVGRRRRP mutant K59A/E61A, pH 8.0, 25°C Schizosaccharomyces pombe
2
-
 SASEAGVRWGRPVGRRRRP mutant H111A, pH 8.0, 25°C Schizosaccharomyces pombe
2
-
 SASEAGVRWGRPVGRRRRP mutant P23A, pH 8.0, 25°C Schizosaccharomyces pombe
2.4
-
 SASEAGVRWGRPVGRRRRP mutant H72A, pH 8.0, 25°C Schizosaccharomyces pombe
3
-
 SASEAGVRWGRPVGRRRRP wild-type, pH 8.0, 25°C Schizosaccharomyces pombe
4.4
-
 SASEAGVRWGRPVGRRRRP mutant K29A, pH 8.0, 25°C Schizosaccharomyces pombe

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0014
-
 pH 8.0, 25°C Schizosaccharomyces pombe CoA-SASEA
0.38
-
 pH 8.0, 25°C Schizosaccharomyces pombe acetonyl-CoA