Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Danio rerio |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + DDDIAAL | Danio rerio | - |
CoA + N-acetyl-DDDIAAL | - |
? | |
acetyl-CoA + EEEIAAL | Danio rerio | best substrate | CoA + N-acetyl-EEEIAAL | - |
? | |
acetyl-CoA + MLGPEGG | Danio rerio | poor substrate | CoA + N-acetyl-MLGPEGG | - |
? | |
acetyl-CoA + SESSSKS | Danio rerio | - |
CoA + N-acetyl-SESSSKS | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | Q7T3B8 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 75 gel filtration | Danio rerio |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | strong expression | Danio rerio | - |
eye | strong expression | Danio rerio | - |
larva | - |
Danio rerio | - |
additional information | ubiquitous expression with especially strong staining in brain and eye | Danio rerio | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + DDDIAAL | - |
Danio rerio | CoA + N-acetyl-DDDIAAL | - |
? | |
acetyl-CoA + DDDIAALRWGRPVGRRRRPVRVYP | - |
Danio rerio | CoA + Nalpha-acetyl-DDDIAALRWGRPVGRRRRPVRVYP | - |
? | |
acetyl-CoA + EEEIAAL | best substrate | Danio rerio | CoA + N-acetyl-EEEIAAL | - |
? | |
acetyl-CoA + EEEIAALRWGRPVGRRRRPVRVYP | - |
Danio rerio | CoA + Nalpha-acetyl-EEEIAALRWGRPVGRRRRPVRVYP | - |
? | |
acetyl-CoA + MLGPEGG | poor substrate | Danio rerio | CoA + N-acetyl-MLGPEGG | - |
? | |
acetyl-CoA + MLGPEGGRWGRPVGRRRRPVRVYP | - |
Danio rerio | CoA + Nalpha-acetyl-MLGPEGGRWGRPVGRRRRPVRVYP | - |
? | |
acetyl-CoA + SESSSKS | - |
Danio rerio | CoA + N-acetyl-SESSSKS | - |
? | |
acetyl-CoA + SESSSKSRWGRPVGRRRRPVRVYP | - |
Danio rerio | CoA + Nalpha-acetyl-SESSSKSRWGRPVGRRRRPVRVYP | - |
? | |
additional information | N-terminal EEEI is acetylated 3fold more than the DDDI substrate, which again is preferred almost 2fold to SESS, the canonical NatA substrate | Danio rerio | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ARD homolog a | - |
Danio rerio |
ARD1 | - |
Danio rerio |
NAA10 | - |
Danio rerio |
Nalpha-acetyltransferase 10 | - |
Danio rerio |
General Information | Comment | Organism |
---|---|---|
malfunction | naa10 morphants display increased lethality, growth retardation and developmental abnormalities like bent axis, abnormal eyes and bent tails | Danio rerio |
physiological function | the enzyme is essential for normal development and viability of zebrafish | Danio rerio |