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Literature summary for 2.3.1.255 extracted from

  • Chang, Y.Y.; Hsu, C.H.
    Structural basis for substrate-specific acetylation of Nalpha-acetyltransferase Ard1 from Sulfolobus solfataricus (2015), Sci. Rep., 5, 8673.
    View publication on PubMed
Search for more literature for 2.3.1.255

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with CoA and with CoA/peptide, sitting drop vapor diffusion method, using 30% (w/v) PEG 4,000, 0.1 M sodium acetate trihydrate, pH 4.6, and 0.2 M (NH4)2SO4 or 5% (w/v) PEG 4,000, 0.1 M sodium acetate trihydrate pH 4.2, and 0.1 M (NH4)2SO4 Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
N132A the mutant shows 4.5fold increase in Km, with no significant difference in kcat compared to the wild type enzyme Saccharolobus solfataricus
R100A the mutant shows 7fold increase in Km, with no significant difference in kcat compared to the wild type enzyme Saccharolobus solfataricus
T105A the mutant shows 3fold increase in Km, with no significant difference in kcat compared to the wild type enzyme Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.196
-
 acetyl-CoA mutant enzyme T105A, at pH 8.0 and 65°C Saccharolobus solfataricus
0.279
-
 acetyl-CoA mutant enzyme R132A, at pH 8.0 and 65°C Saccharolobus solfataricus
0.467
-
 acetyl-CoA mutant enzyme R100A, at pH 8.0 and 65°C Saccharolobus solfataricus
0.6717
-
 acetyl-CoA wild type enzyme, at pH 8.0 and 65°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q980R9
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography and Sephacryl S-200 gel filtration Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + AAKKRG
-
 Saccharolobus solfataricus CoA + N-acetyl-AAKKRG
-
 ?
acetyl-CoA + MEEKVG
-
 Saccharolobus solfataricus CoA + N-acetyl-MEEKVG
-
 ?
acetyl-CoA + SSGTPT
-
 Saccharolobus solfataricus CoA + N-acetyl-SSGTPT
-
 ?

Synonyms

Synonyms Comment Organism
ARD1 subunit of NatA Saccharolobus solfataricus
NatA
-
 Saccharolobus solfataricus
SSO0209 locus name Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.48
-
 acetyl-CoA mutant enzyme R100A, at pH 8.0 and 65°C Saccharolobus solfataricus
0.56
-
 acetyl-CoA wild type enzyme, at pH 8.0 and 65°C Saccharolobus solfataricus
0.56
-
 acetyl-CoA mutant enzyme R132A, at pH 8.0 and 65°C Saccharolobus solfataricus
0.58
-
 acetyl-CoA mutant enzyme T105A, at pH 8.0 and 65°C Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.03
-
 acetyl-CoA mutant enzyme R100A, at pH 8.0 and 65°C Saccharolobus solfataricus
2.05
-
 acetyl-CoA mutant enzyme R132A, at pH 8.0 and 65°C Saccharolobus solfataricus
2.97
-
 acetyl-CoA mutant enzyme T105A, at pH 8.0 and 65°C Saccharolobus solfataricus
8.37
-
 acetyl-CoA wild type enzyme, at pH 8.0 and 65°C Saccharolobus solfataricus