Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
in complex with CoA and with CoA/peptide, sitting drop vapor diffusion method, using 30% (w/v) PEG 4,000, 0.1 M sodium acetate trihydrate, pH 4.6, and 0.2 M (NH4)2SO4 or 5% (w/v) PEG 4,000, 0.1 M sodium acetate trihydrate pH 4.2, and 0.1 M (NH4)2SO4 | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
N132A | the mutant shows 4.5fold increase in Km, with no significant difference in kcat compared to the wild type enzyme | Saccharolobus solfataricus |
R100A | the mutant shows 7fold increase in Km, with no significant difference in kcat compared to the wild type enzyme | Saccharolobus solfataricus |
T105A | the mutant shows 3fold increase in Km, with no significant difference in kcat compared to the wild type enzyme | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.196 | - |
acetyl-CoA | mutant enzyme T105A, at pH 8.0 and 65°C | Saccharolobus solfataricus | |
0.279 | - |
acetyl-CoA | mutant enzyme R132A, at pH 8.0 and 65°C | Saccharolobus solfataricus | |
0.467 | - |
acetyl-CoA | mutant enzyme R100A, at pH 8.0 and 65°C | Saccharolobus solfataricus | |
0.6717 | - |
acetyl-CoA | wild type enzyme, at pH 8.0 and 65°C | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q980R9 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography and Sephacryl S-200 gel filtration | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + AAKKRG | - |
Saccharolobus solfataricus | CoA + N-acetyl-AAKKRG | - |
? | |
acetyl-CoA + MEEKVG | - |
Saccharolobus solfataricus | CoA + N-acetyl-MEEKVG | - |
? | |
acetyl-CoA + SSGTPT | - |
Saccharolobus solfataricus | CoA + N-acetyl-SSGTPT | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ARD1 | subunit of NatA | Saccharolobus solfataricus |
NatA | - |
Saccharolobus solfataricus |
SSO0209 | locus name | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
acetyl-CoA | mutant enzyme R100A, at pH 8.0 and 65°C | Saccharolobus solfataricus | |
0.56 | - |
acetyl-CoA | mutant enzyme R132A, at pH 8.0 and 65°C | Saccharolobus solfataricus | |
0.56 | - |
acetyl-CoA | wild type enzyme, at pH 8.0 and 65°C | Saccharolobus solfataricus | |
0.58 | - |
acetyl-CoA | mutant enzyme T105A, at pH 8.0 and 65°C | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.03 | - |
acetyl-CoA | mutant enzyme R100A, at pH 8.0 and 65°C | Saccharolobus solfataricus | |
2.05 | - |
acetyl-CoA | mutant enzyme R132A, at pH 8.0 and 65°C | Saccharolobus solfataricus | |
2.97 | - |
acetyl-CoA | mutant enzyme T105A, at pH 8.0 and 65°C | Saccharolobus solfataricus | |
8.37 | - |
acetyl-CoA | wild type enzyme, at pH 8.0 and 65°C | Saccharolobus solfataricus |