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Literature summary for 2.3.1.253 extracted from
- Directed evolution of phloroglucinol synthase PhlD with increased stability for phloroglucinol production (2013), Appl. Microbiol. Biotechnol., 97, 5861-5867.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pseudomonas fluorescens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A181S | the mutant shows 110% of wild type specific activity | Pseudomonas fluorescens |
| A82T | the mutant shows wild type specific activity | Pseudomonas fluorescens |
| L136M/S243T | the mutant shows 90% of wild type specific activity | Pseudomonas fluorescens |
| L54V | the mutant shows 110% of wild type specific activity | Pseudomonas fluorescens |
| L54V/A82T | the mutant shows wild type specific activity | Pseudomonas fluorescens |
| M21T | the mutant shows 90% of wild type specific activity | Pseudomonas fluorescens |
| M21T/L54V/A82T | the mutant shows 90% of wild type specific activity | Pseudomonas fluorescens |
| M21T/L54V/A82T/A181S | the mutant shows wild type specific activity | Pseudomonas fluorescens |
| M21T/L54V/A82T/S96R/A181S | the mutant shows 110% of wild type specific activity | Pseudomonas fluorescens |
| M21T/N27D/A82T/A181S | the mutant shows 90% of wild type specific activity | Pseudomonas fluorescens |
| M21T/N27D/L54V/A82T/A181S/S243T | the mutant shows wild type specific activity | Pseudomonas fluorescens |
| M21T/N27D/L54V/A82T/L136M/A181S/S243T | the mutant shows 110% of wild type specific activity | Pseudomonas fluorescens |
| N27D | the mutant shows 90% of wild type specific activity | Pseudomonas fluorescens |
| S96R | the mutant shows 80% of wild type specific activity | Pseudomonas fluorescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3 malonyl-CoA | Pseudomonas fluorescens | - |
phloroglucinol + 3 CO2 + 3 CoA | phloroglucinol is 1,3,5-trihydroxylbenzene | ? |  |
| 3 malonyl-CoA | Pseudomonas fluorescens Pf-5 | - |
phloroglucinol + 3 CO2 + 3 CoA | phloroglucinol is 1,3,5-trihydroxylbenzene | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
| Pseudomonas fluorescens Pf-5 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3 malonyl-CoA | - |
Pseudomonas fluorescens | phloroglucinol + 3 CO2 + 3 CoA | phloroglucinol is 1,3,5-trihydroxylbenzene | ? | |
| 3 malonyl-CoA | - |
Pseudomonas fluorescens Pf-5 | phloroglucinol + 3 CO2 + 3 CoA | phloroglucinol is 1,3,5-trihydroxylbenzene | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PhlD | - |
Pseudomonas fluorescens |
| phloroglucinol synthase | - |
Pseudomonas fluorescens |
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Release 2023.1 (January 2023)
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