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Literature summary for 2.3.1.250 extracted from

  • Proffitt, K.D.; Virshup, D.M.
    Precise regulation of porcupine activity is required for physiological Wnt signaling (2012), J. Biol. Chem., 287, 34167-34178.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.250

Cloned(Commentary)

Cloned (Comment) Organism
gene PORCN, quantitative PCR enzyme expression analysis Mus musculus
gene PORCN, quantitative RT-PCR enzyme expression analysis. PORCN splice variants differ globally in signaling activity in HT-1080 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
H341A site-directed mutagenesis, catalytic active site mutant, Wnt signaling inactive mutant Mus musculus
additional information PORCN is deleted by adenoviral Cre-GFP excision in immortalized MEF cells, PORCN null cells cannot activate WNT3A Mus musculus
additional information zinc finger nuclease-mediated PORCN gene deletion in HT1080 cells. Mutation or skipping of PORCN exon 9 creates cells null for PORCN catalytic activity Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane integral membrane protein, enzyme structure and topology model with cytoplasmic and endoplasmic reticulum luman parts, overview Homo sapiens 5789
-
endoplasmic reticulum membrane integral membrane protein, enzyme structure and topology model with cytoplasmic and endoplasmic reticulum luman parts, overview Mus musculus 5789
-
additional information the enzyme is predicted to palmitoylate Wnt either co-translationally or post-translationally, so that in either case, the catalytic site is likely to be in the membrane or in the lumen of the endoplasmic reticulum Homo sapiens
-
-
additional information the enzyme is predicted to palmitoylate Wnt either co-translationally or post-translationally, so that in either case, the catalytic site is likely to be in the membrane or in the lumen of the endoplasmic reticulum Mus musculus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine Homo sapiens the enzyme catalyzes the palmitoylation of the serine corresponding to Ser209 of Wnt3a CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine Mus musculus the enzyme catalyzes the palmitoylation of the serine corresponding to Ser209 of Wnt3a CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine Homo sapiens
-
 CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine Mus musculus
-
 CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
additional information Homo sapiens PORCN splice variants differ globally in signaling activity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9H237 gene PORCN
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Mus musculus Q9JJJ7
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-

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Mus musculus
-
fibroblast
-
 Mus musculus
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fibrosarcoma cell
-
 Homo sapiens
-
HT-1080 cell
-
 Homo sapiens
-
MEF cell
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine the enzyme catalyzes the palmitoylation of the serine corresponding to Ser209 of Wnt3a Homo sapiens CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine the enzyme catalyzes the palmitoylation of the serine corresponding to Ser209 of Wnt3a Mus musculus CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
-
 Homo sapiens CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
-
 Mus musculus CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
additional information PORCN splice variants differ globally in signaling activity Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
PORCN
-
 Homo sapiens
PORCN
-
 Mus musculus

General Information

General Information Comment Organism
malfunction gene deletion causes embryonic lethality in mice. PORCN null cells cannot activate WNT3A, PORCN null cells do not secrete WNT3A Mus musculus
malfunction mutations in gene PORCN are associated with focal dermal hypoplasia. PORCN null cells are completely incapable of autocrine Wnt signaling Homo sapiens
additional information structure-function analysis, overview Homo sapiens
additional information structure-function analysis, overview Mus musculus
physiological function the enzyme is a membrane-bound O-acyltransferase and is a key non-redundant node for the regulation of global Wnt signaling, essential regulatory role of PORCN in shaping Wnt signaling gradients. All Wnt activity absolutely requires posttranslational modification by the O-acyltransferase PORCN, whose abundance or activity modulates signaling tightly and over a large dynamic range, the enzyme activity is a key modulator of all Wnt ligand activity Homo sapiens
physiological function the enzyme is a membrane-bound O-acyltransferase and is a key non-redundant node for the regulation of global Wnt signaling, essential regulatory role of PORCN in shaping Wnt signaling gradients. All Wnt activity absolutely requires posttranslational modification by the O-acyltransferase PORCN, whose abundance or activity modulates signaling tightly and over a large dynamic range, the enzyme activity is a key modulator of all Wnt ligand activity Mus musculus