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Literature summary for 2.3.1.248 extracted from

  • Luo, J.; Fuell, C.; Parr, A.; Hill, L.; Bailey, P.; Elliott, K.; Fairhurst, S.A.; Martin, C.; Michael, A.J.
    A novel polyamine acyltransferase responsible for the accumulation of spermidine conjugates in Arabidopsis seed (2009), Plant Cell, 21, 318-333.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene At2g23510, quantitative real-time RT-PCR expression analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 Rosetta (DE3) pLysS Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of a T-DNA insertion mutant SM_3_38374, complementation of At2g23510 knockout mutant phenotype by overexpression of wild-type gene At2g23510 under control of CMV 35S promoter using Agrobacterium tumefaciens strain GV3101 transfrmation method. Transcript of At2g23510 can be detected in siliques from wild-type plants but not in those from the insertion line, confirming that the insertion eliminates production of the At2g23510 transcript Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0083
-
sinapoyl-CoA pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
0.0346
-
N1,N8-bis(sinapoyl)-spermidine pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
0.0374
-
spermidine pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
0.0836
-
CoA pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
0.2369
-
putrescine pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 sinapoyl-CoA + spermidine Arabidopsis thaliana
-
2 CoA + N1,N8-bis(sinapoyl)-spermidine identification of two derivatives, i.e. N-((4'-O-glycosyl)-sinapoyl),N'-sinapoylspermidine and N1,N8-di(sinapoyl)-spermidine, in seeds by NMR analysis r
2 sinapoyl-CoA + spermidine Arabidopsis thaliana Col-0
-
2 CoA + N1,N8-bis(sinapoyl)-spermidine identification of two derivatives, i.e. N-((4'-O-glycosyl)-sinapoyl),N'-sinapoylspermidine and N1,N8-di(sinapoyl)-spermidine, in seeds by NMR analysis r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O80467 gene At2g23510
-
Arabidopsis thaliana Col-0 O80467 gene At2g23510
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21 Rosetta (DE3) pLysS by nickel affinity chromatography and desalting gel filtration Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
seed the enzyme is expressed mainly in seeds Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-
silique
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 CoA + N1,N8-bis(sinapoyl)-spermidine
-
Arabidopsis thaliana 2 sinapoyl-CoA + spermidine
-
r
2 CoA + N1,N8-bis(sinapoyl)-spermidine
-
Arabidopsis thaliana Col-0 2 sinapoyl-CoA + spermidine
-
r
2 sinapoyl-CoA + putrescine
-
Arabidopsis thaliana ?
-
r
2 sinapoyl-CoA + putrescine
-
Arabidopsis thaliana Col-0 ?
-
r
2 sinapoyl-CoA + spermidine
-
Arabidopsis thaliana 2 CoA + N1,N8-bis(sinapoyl)-spermidine
-
r
2 sinapoyl-CoA + spermidine
-
Arabidopsis thaliana 2 CoA + N1,N8-bis(sinapoyl)-spermidine identification of two derivatives, i.e. N-((4'-O-glycosyl)-sinapoyl),N'-sinapoylspermidine and N1,N8-di(sinapoyl)-spermidine, in seeds by NMR analysis r
2 sinapoyl-CoA + spermidine
-
Arabidopsis thaliana Col-0 2 CoA + N1,N8-bis(sinapoyl)-spermidine
-
r
2 sinapoyl-CoA + spermidine
-
Arabidopsis thaliana Col-0 2 CoA + N1,N8-bis(sinapoyl)-spermidine identification of two derivatives, i.e. N-((4'-O-glycosyl)-sinapoyl),N'-sinapoylspermidine and N1,N8-di(sinapoyl)-spermidine, in seeds by NMR analysis r
additional information no activity with acyl donors coumaroyl-CoA, caffeoyl-CoA, and feruloyl-CoA, and also no activity with acyl acceptors cadaverine, norspermidine, spermine, homospermidine, agmatine, and tyramine, low activity with putrescine Arabidopsis thaliana ?
-
?
additional information no activity with acyl donors coumaroyl-CoA, caffeoyl-CoA, and feruloyl-CoA, and also no activity with acyl acceptors cadaverine, norspermidine, spermine, homospermidine, agmatine, and tyramine, low activity with putrescine Arabidopsis thaliana Col-0 ?
-
?

Synonyms

Synonyms Comment Organism
BAHD acyl transferase
-
Arabidopsis thaliana
SDT
-
Arabidopsis thaliana
spermidine sinapoyl CoA acyltransferase
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
putrescine pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
5.1
-
sinapoyl-CoA pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
5.6
-
spermidine pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
37.8
-
N1,N8-bis(sinapoyl)-spermidine pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
39.6
-
CoA pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
recombinant enzyme, reverse reaction Arabidopsis thaliana
9
-
recombinant enzyme, forward reaction Arabidopsis thaliana

General Information

General Information Comment Organism
evolution the enzyme is a member of the BAHD acyltransferase family Arabidopsis thaliana
malfunction enzyme T-DNA Insertion knockout mutant SM_3_38374 phenotype, metabolic profiling, overview Arabidopsis thaliana
physiological function spermidine sinapoyl CoA acyltransferase is required for the production of disinapoyl spermidine and its glucoside in Arabidopsis seeds. The sinapoyl spermidine derivatives, N-((4'-O-glycosyl)-sinapoyl),N'-sinapoylspermidine and N,N'-disinapoylspermidine, are the two major polyamine conjugates that accumulate in Arabidopsis thaliana seeds, metabolic profiling Arabidopsis thaliana