Cloned (Comment) | Organism |
---|---|
gene At2g23510, quantitative real-time RT-PCR expression analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 Rosetta (DE3) pLysS | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a T-DNA insertion mutant SM_3_38374, complementation of At2g23510 knockout mutant phenotype by overexpression of wild-type gene At2g23510 under control of CMV 35S promoter using Agrobacterium tumefaciens strain GV3101 transfrmation method. Transcript of At2g23510 can be detected in siliques from wild-type plants but not in those from the insertion line, confirming that the insertion eliminates production of the At2g23510 transcript | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0083 | - |
sinapoyl-CoA | pH 7.3, 30°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
0.0346 | - |
N1,N8-bis(sinapoyl)-spermidine | pH 7.3, 30°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
0.0374 | - |
spermidine | pH 7.3, 30°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
0.0836 | - |
CoA | pH 7.3, 30°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
0.2369 | - |
putrescine | pH 7.3, 30°C, recombinant His6-tagged enzyme | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 sinapoyl-CoA + spermidine | Arabidopsis thaliana | - |
2 CoA + N1,N8-bis(sinapoyl)-spermidine | identification of two derivatives, i.e. N-((4'-O-glycosyl)-sinapoyl),N'-sinapoylspermidine and N1,N8-di(sinapoyl)-spermidine, in seeds by NMR analysis | r | |
2 sinapoyl-CoA + spermidine | Arabidopsis thaliana Col-0 | - |
2 CoA + N1,N8-bis(sinapoyl)-spermidine | identification of two derivatives, i.e. N-((4'-O-glycosyl)-sinapoyl),N'-sinapoylspermidine and N1,N8-di(sinapoyl)-spermidine, in seeds by NMR analysis | r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O80467 | gene At2g23510 | - |
Arabidopsis thaliana Col-0 | O80467 | gene At2g23510 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21 Rosetta (DE3) pLysS by nickel affinity chromatography and desalting gel filtration | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | the enzyme is expressed mainly in seeds | Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 CoA + N1,N8-bis(sinapoyl)-spermidine | - |
Arabidopsis thaliana | 2 sinapoyl-CoA + spermidine | - |
r | |
2 CoA + N1,N8-bis(sinapoyl)-spermidine | - |
Arabidopsis thaliana Col-0 | 2 sinapoyl-CoA + spermidine | - |
r | |
2 sinapoyl-CoA + putrescine | - |
Arabidopsis thaliana | ? | - |
r | |
2 sinapoyl-CoA + putrescine | - |
Arabidopsis thaliana Col-0 | ? | - |
r | |
2 sinapoyl-CoA + spermidine | - |
Arabidopsis thaliana | 2 CoA + N1,N8-bis(sinapoyl)-spermidine | - |
r | |
2 sinapoyl-CoA + spermidine | - |
Arabidopsis thaliana | 2 CoA + N1,N8-bis(sinapoyl)-spermidine | identification of two derivatives, i.e. N-((4'-O-glycosyl)-sinapoyl),N'-sinapoylspermidine and N1,N8-di(sinapoyl)-spermidine, in seeds by NMR analysis | r | |
2 sinapoyl-CoA + spermidine | - |
Arabidopsis thaliana Col-0 | 2 CoA + N1,N8-bis(sinapoyl)-spermidine | - |
r | |
2 sinapoyl-CoA + spermidine | - |
Arabidopsis thaliana Col-0 | 2 CoA + N1,N8-bis(sinapoyl)-spermidine | identification of two derivatives, i.e. N-((4'-O-glycosyl)-sinapoyl),N'-sinapoylspermidine and N1,N8-di(sinapoyl)-spermidine, in seeds by NMR analysis | r | |
additional information | no activity with acyl donors coumaroyl-CoA, caffeoyl-CoA, and feruloyl-CoA, and also no activity with acyl acceptors cadaverine, norspermidine, spermine, homospermidine, agmatine, and tyramine, low activity with putrescine | Arabidopsis thaliana | ? | - |
? | |
additional information | no activity with acyl donors coumaroyl-CoA, caffeoyl-CoA, and feruloyl-CoA, and also no activity with acyl acceptors cadaverine, norspermidine, spermine, homospermidine, agmatine, and tyramine, low activity with putrescine | Arabidopsis thaliana Col-0 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BAHD acyl transferase | - |
Arabidopsis thaliana |
SDT | - |
Arabidopsis thaliana |
spermidine sinapoyl CoA acyltransferase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
putrescine | pH 7.3, 30°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
5.1 | - |
sinapoyl-CoA | pH 7.3, 30°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
5.6 | - |
spermidine | pH 7.3, 30°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
37.8 | - |
N1,N8-bis(sinapoyl)-spermidine | pH 7.3, 30°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
39.6 | - |
CoA | pH 7.3, 30°C, recombinant His6-tagged enzyme | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme, reverse reaction | Arabidopsis thaliana |
9 | - |
recombinant enzyme, forward reaction | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the BAHD acyltransferase family | Arabidopsis thaliana |
malfunction | enzyme T-DNA Insertion knockout mutant SM_3_38374 phenotype, metabolic profiling, overview | Arabidopsis thaliana |
physiological function | spermidine sinapoyl CoA acyltransferase is required for the production of disinapoyl spermidine and its glucoside in Arabidopsis seeds. The sinapoyl spermidine derivatives, N-((4'-O-glycosyl)-sinapoyl),N'-sinapoylspermidine and N,N'-disinapoylspermidine, are the two major polyamine conjugates that accumulate in Arabidopsis thaliana seeds, metabolic profiling | Arabidopsis thaliana |