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Literature summary for 2.3.1.246 extracted from

  • Wu, H.C.; Li, Y.S.; Liu, Y.C.; Lyu, S.Y.; Wu, C.J.; Li, T.L.
    Chain elongation and cyclization in type III PKS DpgA (2012), Chembiochem, 13, 862-871.
    View publication on PubMed
Search for more literature for 2.3.1.246

Cloned(Commentary)

Cloned (Comment) Organism
-
 Amycolatopsis orientalis

Protein Variants

Protein Variants Comment Organism
C160A/C190A formation of (3,5-dihydroxyphenylacetyl)-CoA is barely detectable Amycolatopsis orientalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41593
-
 2 * 41593, electrospray mass spectrometry Amycolatopsis orientalis

Organism

Organism UniProt Comment Textmining
Amycolatopsis orientalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Amycolatopsis orientalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 malonyl-CoA diketyl-CoA and triketyl-CoA are confirmed to be the intermediates in the enzyme catalyzed synthesis of (3,5-dihydroxyphenylacetyl)-CoA. Polyketone intermediates alternate between the Cys160 residue and CoA through transthioesterification reactions, in which the chain is elongated by enolate attack on the thioester carbon of the enzyme-bound (poly)ketide intermediate by decarboxylation in malonyl-CoA or polyketide-CoA. Polyketone cyclization likely arises on CoA but not on the enzyme. Experimental data suggest that cyclization of linear tetraketidyl-CoA occurs in concert with or before decarboxylation of the terminal carboxyl group Amycolatopsis orientalis (3,5-dihydroxyphenylacetyl)-CoA + 3 CoA + 4 CO2 + H2O
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 ?

Subunits

Subunits Comment Organism
dimer 2 * 41593, electrospray mass spectrometry Amycolatopsis orientalis

Synonyms

Synonyms Comment Organism
dpgA
-
 Amycolatopsis orientalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Amycolatopsis orientalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Amycolatopsis orientalis