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Literature summary for 2.3.1.246 extracted from

  • Tseng, C.C.; McLoughlin, S.M.; Kelleher, N.L.; Walsh, C.T.
    Role of the active site cysteine of DpgA, a bacterial type III polyketide synthase (2004), Biochemistry, 43, 970-980.
    View publication on PubMed
Search for more literature for 2.3.1.246

Protein Variants

Protein Variants Comment Organism
C160A kcat/Km for malonyl-CoA is 0.16% compared to the kcat/Km-value of the wild-type enzyme. The mutant shows an increased partition ratio for malonyl-CoA decarboxylation to acetyl-CoA vs condensation to 3,5-dihydroxyphenylacetyl-CoA, reflecting more uncoupling in the mutant enzyme Amycolatopsis mediterranei
C160S kcat/Km for malonyl-CoA is 2.3% compared to the kcat/Km-value of the wild-type enzyme Amycolatopsis mediterranei
C190A mutant retains wild-type activity Amycolatopsis mediterranei
H296A mutant has a 6fold drop in kcat Amycolatopsis mediterranei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 malonyl-CoA pH 7.5, 24°C, wild-type enzyme Amycolatopsis mediterranei
0.13
-
 malonyl-CoA pH 7.5, 24°C, mutant enzyme C160S Amycolatopsis mediterranei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4 malonyl-CoA Amycolatopsis mediterranei the enzyme is involved in biosynthesis of 3,5-dihydroxyphenylglycine, a key nonproteinogenic residue in the vancomycin family of antibiotics 3,5-dihydroxyphenylacetyl-CoA + 3 CoA + 4 CO2 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Amycolatopsis mediterranei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Amycolatopsis mediterranei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 malonyl-CoA the enzyme is involved in biosynthesis of 3,5-dihydroxyphenylglycine, a key nonproteinogenic residue in the vancomycin family of antibiotics Amycolatopsis mediterranei 3,5-dihydroxyphenylacetyl-CoA + 3 CoA + 4 CO2 + H2O
-
 ?
4 malonyl-CoA
-
 Amycolatopsis mediterranei 3,5-dihydroxyphenylacetyl-CoA + 3 CoA + 4 CO2 + H2O
-
 ?

Synonyms

Synonyms Comment Organism
dpgA
-
 Amycolatopsis mediterranei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
24
-
 assay at Amycolatopsis mediterranei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0029
-
 malonyl-CoA pH 7.5, 24°C, mutant enzyme C160S Amycolatopsis mediterranei
0.0135
-
 malonyl-CoA pH 7.5, 24°C, wild-type enzyme Amycolatopsis mediterranei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Amycolatopsis mediterranei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.02
-
 malonyl-CoA pH 7.5, 24°C, mutant enzyme C160S Amycolatopsis mediterranei
0.9
-
 malonyl-CoA pH 7.5, 24°C, wild-type enzyme Amycolatopsis mediterranei