Cloned (Comment) | Organism |
---|---|
gene CERS6, recombinant expression of N-terminally FLAG-tagged CerS6 and EGFP-tagged CerS6 in SW-480 cells and localization in the perinuclear region. When cells transfected with pCerS6-IRES2-EGFP are treated with TRAIL, GFP positive cells appear to be undergoing apoptosis. Cells stably transfected with CerS6 but not with the empty plasmid acquire a TRAIL sensitive phenotype | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | - |
Homo sapiens | 5789 | - |
perinuclear space | recombinant FLAG-tagged CerS6 localizes primarily to the perinuclear region | Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphinganine + palmitoyl-CoA | Homo sapiens | - |
N-palmitoylsphinganine + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q6ZMG9 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colonic cancer cell | - |
Homo sapiens | - |
intestine | high expression level of CerS6 | Homo sapiens | - |
SW-480 cell | - |
Homo sapiens | - |
SW-620 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphinganine + palmitoyl-CoA | - |
Homo sapiens | N-palmitoylsphinganine + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ceramide synthase 6 | - |
Homo sapiens |
CerS6 | - |
Homo sapiens |
Lass6 | - |
Homo sapiens |
longevity assurance homolog 6 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | RNAi against CerS6 results in a specific decrease in intracellular C16-ceramide and protects SW-480 cells against TRAIL-mediated apoptosis while increasing CerS6 expression sensitizes SW-620 cells to TRAIL. Downregulation of CerS6 does not interfere with caspase activation but appears to inhibit translocation of activated caspase-3 into the nucleus | Homo sapiens |
additional information | recombinant FLAG-tagged CerS6 localizes primarily to the perinuclear region | Homo sapiens |
physiological function | ceramide synthase 6 modulates TRAIL sensitivity and nuclear translocation of active caspase-3 in colon cancer cells. Ceramide synthase 6 (CerS6 also known as longevity assurance homolog 6/LASS6) preferentially generates C16-ceramide and can influence TRAIL susceptibility. CerS6 may regulate events at the nuclear membrane and allow late stage apoptotic signaling | Homo sapiens |