Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.24 extracted from

  • Shimeno, H.; Soeda, S.; Sakamoto, M.; Kouchi, T.; Kowakame, T.; Kihara, T.
    Partial purification and characterization of sphingosine N-acyltransferase (ceramide synthase) from bovine liver mitochondrion-rich fraction (1998), Lipids, 33, 601-605 .
    View publication on PubMed
Search for more literature for 2.3.1.24

Inhibitors

Inhibitors Comment Organism Structure
3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate
-
 Bos taurus
Triton X-100
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics and substrate specificity, overview Bos taurus
0.141
-
 palmitoyl-CoA pH 7.5, 37°C Bos taurus
0.144
-
 sphinganine pH 7.5, 37°C Bos taurus
0.146
-
 stearoyl-CoA pH 7.5, 37°C Bos taurus
0.171
-
 sphingosine pH 7.5, 37°C Bos taurus
0.18
-
 oleoyl-CoA pH 7.5, 37°C Bos taurus
0.299
-
 behenoyl-CoA pH 7.5, 37°C Bos taurus
0.358
-
 lauroyl-CoA pH 7.5, 37°C Bos taurus
0.522
-
 hexanoyl-CoA pH 7.5, 37°C Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial membrane
-
 Bos taurus 31966
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
240000 260000 gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus A0A3Q1MVP2
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 98.7fold from liver mitochondrionrich fraction by solubilization with n-octyl beta-D-thioglucoside, ion exchange chromatography, sphingosine affinity chromatography, and gel filtration Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0148
-
 purified enzyme, pH 7.5, 37°C Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity of the purified enzyme is determined with various acyl (C6-C22)-CoA and sphingosine or with stearoyl-CoA and sphinganine as substrates. CoA esters having regular chain (C16-C18) are good substrates. Very long chain (C22) or short chain (C6-C12) show low activity Bos taurus ?
-
 ?
sphinganine + stearoyl-CoA
-
 Bos taurus N-stearoylsphinganine + CoA
-
 ?
sphingosine + behenoyl-CoA low activity Bos taurus N-behenoylsphingosine + CoA
-
 ?
sphingosine + hexanoyl-CoA very low activity Bos taurus N-hexanoylsphingosine + CoA
-
 ?
sphingosine + lauroyl-CoA very low activity Bos taurus N-lauroylsphingosine + CoA
-
 ?
sphingosine + oleoyl-CoA
-
 Bos taurus N-oleoylsphingosine + CoA
-
 ?
sphingosine + palmitoyl-CoA
-
 Bos taurus N-palmitoylsphingosine + CoA
-
 ?
sphingosine + stearoyl-CoA
-
 Bos taurus N-stearoylsphingosine + CoA
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 62000-72000, SDS-PAGE Bos taurus

Synonyms

Synonyms Comment Organism
ceramide synthase
-
 Bos taurus
ceramide synthase 5
-
 Bos taurus
sphingosine N-acyltransferase
-
 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
7 8 over 70% of maximal activity within this range Bos taurus