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Literature summary for 2.3.1.24 extracted from

  • Xu, Z.; Zhou, J.; McCoy, D.M.; Mallampalli, R.K.
    LASS5 is the predominant ceramide synthase isoform involved in de novo sphingolipid synthesis in lung epithelia (2005), J. Lipid Res., 46, 1229-1238 .
    View publication on PubMed
Search for more literature for 2.3.1.24

Cloned(Commentary)

Cloned (Comment) Organism
gene Cers5, transient recombinant expression of His-tagged LASS5 in MLE cells Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information LASS5 gene silencing by siRNAexogenously expressed LASS5 in lung epithelia is membrane-associated, triggering increased ceramide synthesis. Maximal inhibition is achieved when LASS5 is coexpressed with a plasmid encoding a neutral sphingomyelinase involved in sphingomyelin hydrolysis. Compared with control cells, cells transfected with LASS5 siRNA exhibit a 43% increase in rates of phosphatidylcholine synthesis Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
fumonisin B1 knockdown using fumonisin B 1 reduces ceramide synthase activity by 78% Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-associated Mus musculus 16020
-

Organism

Organism UniProt Comment Textmining
Mus musculus Q9D6K9
-
-
Mus musculus C57BL/6 Q9D6K9
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged LASS5 from MLE cells by nickel affinity chromatography and ultrafiltration Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
epithelium
-
 Mus musculus
-
fibroblast
-
 Mus musculus
-
lung high enzyme expression level, pulmonary LASS5 is developmentally regulated, but its expression is spatially and gender-nonspecific Mus musculus
-
MLE-12 cell mouse lung epithelial cell Mus musculus
-
additional information in primary type II epithelial cells, murine lung fibroblasts, and MLE cells, LASS5 is the predominant ceramide synthase species identified Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphinganine + palmitoyl-CoA
-
 Mus musculus N-palmitoylsphinganine + CoA
-
 ?
sphinganine + palmitoyl-CoA
-
 Mus musculus C57BL/6 N-palmitoylsphinganine + CoA
-
 ?
sphingosine + palmitoyl-CoA
-
 Mus musculus N-palmitoylsphingosine + CoA
-
 ?
sphingosine + palmitoyl-CoA
-
 Mus musculus C57BL/6 N-palmitoylsphingosine + CoA
-
 ?

Synonyms

Synonyms Comment Organism
ceramide synthase 5
-
 Mus musculus
CerS5
-
 Mus musculus
LASS5
-
 Mus musculus
longevity assurance homolog 5
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mus musculus

General Information

General Information Comment Organism
malfunction knockdown using fumonisin B 1 or LASS5 small, interfering RNA reduced ceramide synthase activity by 78% or 45%, respectively. Exogenously expressed LASS5 in lung epithelia is membrane-associated, triggering increased ceramide synthesis. Compared with control cells, cells transfected with LASS5 siRNA exhibit a 43% increase in rates of phosphatidylcholine synthesis Mus musculus
metabolism LASS5 expression alone reduces choline incorporation into phosphatidylcholine by 26%, whereas coexpression of LASS5 with sphingomyelinase produces a nearly 40% reduction in phosphatidylcholine synthesis Mus musculus
physiological function LASS5 is the major ceramide synthase gene product involved in sphingolipid production that may also regulate PtdCho metabolism in pulmonary epithelia. LASS5 regulates surfactant phosphatidylcholine (PtdCho) synthesis Mus musculus