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Literature summary for 2.3.1.24 extracted from

  • Figueiredo, J.M.; Rodrigues, D.C.; Silva, R.C.; Koeller, C.M.; Jiang, J.C.; Jazwinski, S.M.; Previato, J.O.; Mendonca-Previato, L.; Urmenyi, T.P.; Heise, N.
    Molecular and functional characterization of the ceramide synthase from Trypanosoma cruzi (2012), Mol. Biochem. Parasitol., 182, 62-74.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.24

Activating Compound

Activating Compound Comment Organism Structure
CHAPS required Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
fumonisin B1
-
 Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0124
-
 palmitoyl-CoA pH 7.5, 28°C Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome no reverse ceramidase activity is detected in Trypanosoma cruzi microsomal fractions Trypanosoma cruzi
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the change of a Tris-Mg2+-DTT-NaF-NADPH-CHAPS buffer to a phosphate/K+-Mn2+-CHAPS buffer slightly improves the efficiency of Cer formation Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi G0YYC4 Trypanosoma cruzi microsomes harbor a Fumonisin B1-sensitive acyl-CoA-dependent CerS activity that uses both dihydrosphingosine and sphingosine as long-chain sphingoid base acceptors and palmitoyl-CoA as the principal fatty-acyl-CoA substrate donor
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information palmitoyl-CoAis the preferred substrate. Low activity towards myristoyl-CoA and stearoyl-CoA, and almost no CerS activity associated with all other acyl-CoAs tested, which range from C3, C4 and to C20 up to C26 Trypanosoma cruzi ?
-
 ?
palmitoyl-CoA + dihydrosphingosine no reverse ceramidase activity is detected in Trypanosoma cruzi microsomal fractions Trypanosoma cruzi CoA + N-palmitoyldihydrosphingosine
-
 ?
palmitoyl-CoA + sphingosine no reverse ceramidase activity is detected in Trypanosoma cruzi microsomal fractions Trypanosoma cruzi CoA + N-palmitoylsphingosine
-
 ?

Synonyms

Synonyms Comment Organism
CerS
-
 Trypanosoma cruzi
TcCerS
-
 Trypanosoma cruzi
TcCERS1
-
 Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
14 28 TcCerS from Y strain has an optimal activity that increases between 14°C and 28°C, but decreases at 37°C and 42°C Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Trypanosoma cruzi

pH Range

pH Minimum pH Maximum Comment Organism
7 7.5 optimal pH Trypanosoma cruzi

General Information

General Information Comment Organism
malfunction the TcCERS1 gene is able to functionally complement the lethality of a lag1DELTA lac1DELTA double deletion yeast mutant in which the acyl-CoA-dependent CerS is not detectable Trypanosoma cruzi