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Literature summary for 2.3.1.236 extracted from

  • Mori, S.; Simkhada, D.; Zhang, H.; Erb, M.S.; Zhang, Y.; Williams, H.; Fedoseyenko, D.; Russell, W.K.; Kim, D.; Fleer, N.; Ealick, S.E.; Watanabe, C.M.
    Polyketide ring expansion mediated by a thioesterase, chain elongation and cyclization domain, in azinomycin biosynthesis: characterization of AziB and AziG (2016), Biochemistry, 55, 704-714.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces sahachiroi

Organism

Organism UniProt Comment Textmining
Streptomyces sahachiroi B4XYB8
-
-
Streptomyces sahachiroi NRRL 2485 B4XYB8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 5 malonyl-CoA + 3 NADPH + 3 H+
-
 Streptomyces sahachiroi 5-methyl-1-naphthoate + 6 CoA + 5 CO2 + 4 H2O + 3 NADP+ incubation of substrates with isoform AziB alone leads to a truncated single-ring product, 2-methylbenzoic acid. Presence of thioesterase AziG acting as a chain elongation and cyclization domain is required for additional two rounds of chain extension to form the expected product, 5-methyl-1-naphthoate. None of the other thioesterase domains (AziA6, AziA7, AziA8) are capable of catalyzing the reaction ?
acetyl-CoA + 5 malonyl-CoA + 3 NADPH + 3 H+
-
 Streptomyces sahachiroi NRRL 2485 5-methyl-1-naphthoate + 6 CoA + 5 CO2 + 4 H2O + 3 NADP+ incubation of substrates with isoform AziB alone leads to a truncated single-ring product, 2-methylbenzoic acid. Presence of thioesterase AziG acting as a chain elongation and cyclization domain is required for additional two rounds of chain extension to form the expected product, 5-methyl-1-naphthoate. None of the other thioesterase domains (AziA6, AziA7, AziA8) are capable of catalyzing the reaction ?

Synonyms

Synonyms Comment Organism
AziB
-
 Streptomyces sahachiroi