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Literature summary for 2.3.1.236 extracted from

  • Zhao, Q.; He, Q.; Ding, W.; Tang, M.; Kang, Q.; Yu, Y.; Deng, W.; Zhang, Q.; Fang, J.; Tang, G.; Liu, W.
    Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis (2008), Chem. Biol., 15, 693-705.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression of aziB in Streptomyces albus. Addition of aziB1 (encoding a P450 hydroxylase) and aziB2 (encoding an O-methyltransferase) to aziB in Streptomyces albus allows for the production of 3-methoxy-5-methyl-naohthoic acid as the first building block for skeleton assembly of azinomycin B Streptomyces sahachiroi

Protein Variants

Protein Variants Comment Organism
additional information constructs harboring mutant aziB, in which site specific mutations are performed for inactivating the KR and DH domains, respectively, are introduced into Streptomyces albus, yielding the recombinant strain AL1007 (to express the KR mutant AziB, G1398A within the NADPH-binding motif GxGxxG), AL1008 (to express the KR mutant AziB, Y1549F at the conserved active site), and AL1009 (to express the DH mutant AziB, H935F within the conserved motif HxxxGxxxxP). Upon HPLC-MS analysis, AL1007, AL1008, and AL1009 fail to produce 5-methyl-NPA, confirming that the reductive and dehydrating actions governed by the KR and DH domains of AziB are essential for the 5-methyl-naphthoic acid formation Streptomyces sahachiroi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + 5 malonyl-CoA + 3 NADPH + 3 H+ Streptomyces sahachiroi the multi-domain polyketide synthase involved in the synthesis of azinomycin B 5-methyl-1-naphthoate + 6 CoA + 5 CO2 + 4 H2O + 3 NADP+
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Organism

Organism UniProt Comment Textmining
Streptomyces sahachiroi B4XYB8
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 5 malonyl-CoA + 3 NADPH + 3 H+
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Streptomyces sahachiroi 5-methyl-1-naphthoate + 6 CoA + 5 CO2 + 4 H2O + 3 NADP+
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acetyl-CoA + 5 malonyl-CoA + 3 NADPH + 3 H+ the multi-domain polyketide synthase involved in the synthesis of azinomycin B Streptomyces sahachiroi 5-methyl-1-naphthoate + 6 CoA + 5 CO2 + 4 H2O + 3 NADP+
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Synonyms

Synonyms Comment Organism
5-methyl-naphthoic acid synthase
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Streptomyces sahachiroi
AziB
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Streptomyces sahachiroi
NPA synthase
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Streptomyces sahachiroi

General Information

General Information Comment Organism
metabolism the multi-domain polyketide synthase involved in the synthesis of azinomycin B Streptomyces sahachiroi