Cloned (Comment) | Organism |
---|---|
heterologous expression of aziB in Streptomyces albus. Addition of aziB1 (encoding a P450 hydroxylase) and aziB2 (encoding an O-methyltransferase) to aziB in Streptomyces albus allows for the production of 3-methoxy-5-methyl-naohthoic acid as the first building block for skeleton assembly of azinomycin B | Streptomyces sahachiroi |
Protein Variants | Comment | Organism |
---|---|---|
additional information | constructs harboring mutant aziB, in which site specific mutations are performed for inactivating the KR and DH domains, respectively, are introduced into Streptomyces albus, yielding the recombinant strain AL1007 (to express the KR mutant AziB, G1398A within the NADPH-binding motif GxGxxG), AL1008 (to express the KR mutant AziB, Y1549F at the conserved active site), and AL1009 (to express the DH mutant AziB, H935F within the conserved motif HxxxGxxxxP). Upon HPLC-MS analysis, AL1007, AL1008, and AL1009 fail to produce 5-methyl-NPA, confirming that the reductive and dehydrating actions governed by the KR and DH domains of AziB are essential for the 5-methyl-naphthoic acid formation | Streptomyces sahachiroi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 5 malonyl-CoA + 3 NADPH + 3 H+ | Streptomyces sahachiroi | the multi-domain polyketide synthase involved in the synthesis of azinomycin B | 5-methyl-1-naphthoate + 6 CoA + 5 CO2 + 4 H2O + 3 NADP+ | - |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sahachiroi | B4XYB8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 5 malonyl-CoA + 3 NADPH + 3 H+ | - |
Streptomyces sahachiroi | 5-methyl-1-naphthoate + 6 CoA + 5 CO2 + 4 H2O + 3 NADP+ | - |
? | |
acetyl-CoA + 5 malonyl-CoA + 3 NADPH + 3 H+ | the multi-domain polyketide synthase involved in the synthesis of azinomycin B | Streptomyces sahachiroi | 5-methyl-1-naphthoate + 6 CoA + 5 CO2 + 4 H2O + 3 NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-methyl-naphthoic acid synthase | - |
Streptomyces sahachiroi |
AziB | - |
Streptomyces sahachiroi |
NPA synthase | - |
Streptomyces sahachiroi |
General Information | Comment | Organism |
---|---|---|
metabolism | the multi-domain polyketide synthase involved in the synthesis of azinomycin B | Streptomyces sahachiroi |