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Literature summary for 2.3.1.235 extracted from
- Reconstitution of the iterative type II polyketide synthase for tetracenomycin F2 biosynthesis (1998), Biochemistry, 37, 8132-8138.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of the tcmKL genes together in Streptomyces lividans to obtain the KS subunits | Streptomyces glaucescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10 malonyl-CoA | Streptomyces glaucescens | the multi-domain polyketide synthase involved in the synthesis of tetracenomycin | tetracenomycin F2 + 10 CoA + 10 CO2 + 2 H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces glaucescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of the TcmK/TcmL alphabeta complex | Streptomyces glaucescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10 malonyl-CoA | the multi-domain polyketide synthase involved in the synthesis of tetracenomycin | Streptomyces glaucescens | tetracenomycin F2 + 10 CoA + 10 CO2 + 2 H2O | - |
? | |
| 10 malonyl-CoA | the multi-domain polyketide synthase involves a ketosynthase complex (TcmKL), an acyl carrier protein (TcmM), a malonyl CoA:ACP acyltransferase (MAT), and a cyclase (TcmN). A malonyl-CoA molecule is initially bound to the acyl carrier protein and decarboxylated to form an acetyl starter unit. Additional two-carbon units are added from nine more malonyl-CoA molecules | Streptomyces glaucescens | tetracenomycin F2 + 10 CoA + 10 CO2 + 2 H2O | tetracenomycin F2 i.e. 4-(3-acetyl-4,5,7,10-tetrahydroxyanthracen-2-yl)-3-oxobutanoic acid | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the TcmKL KS alphabeta ketosynthase complex, an acyl carrier protein TcmM ACP, TcmN cyclase, and FabD malonyl CoA:ACP acyltransferase proteins constitute the minimal requirements for a functional tetracenomycin polyketide synthase in vitro | Streptomyces glaucescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TCM F2 | - |
Streptomyces glaucescens |
| TCM PKS | - |
Streptomyces glaucescens |
| type II polyketide synthase | - |
Streptomyces glaucescens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the multi-domain polyketide synthase involved in the synthesis of tetracenomycin | Streptomyces glaucescens |
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