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Literature summary for 2.3.1.233 extracted from
- Properties and substrate specificity of RppA, a chalcone synthase-related polyketide synthase in Streptomyces griseus (2002), J. Biol. Chem., 277, 4628-4635.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Streptomyces griseus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.93 | - |
malonyl-CoA | in 100 mM Tris-HCl (pH 7.5), 1 mM EDTA, 1 mM 2-mercaptoethanol, at 30°C | Streptomyces griseus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5 malonyl-CoA | Streptomyces griseus | - |
1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces griseus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Streptomyces griseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5 malonyl-CoA | - |
Streptomyces griseus | 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O | - |
? | |
| acetoacetyl-CoA | 100% activity | Streptomyces griseus | 4-hydroxy-6-methyl-2-pyrone + CoA + CO2 + H2O | - |
? | |
| acetoacetyl-CoA + methylmalonyl-CoA | - |
Streptomyces griseus | 3,6-dimethyl-4-hydroxy-2-pyrone + CoA + CO2 + H2O | - |
? | |
| butyryl-CoA | 21% activity compared to acetoacetyl-CoA | Streptomyces griseus | 4-hydroxy-6-propyl-2-pyrone + 4-hydroxy-6-(2'-oxopentyl)-2-pyrone + 1,3,6,8-tetrahydroxynaphthalene + CoA + CO2 + H2O | - |
? | |
| hexanoyl-CoA | 43% activity compared to acetoacetyl-CoA | Streptomyces griseus | 4-hydroxy-6-pentyl-2-pyrone + 4-hydroxy-6-(2'-oxoheptyl)-2-pyrone + CoA + CO2 + H2O | - |
? | |
| isobutyryl-CoA | 7% activity compared to acetoacetyl-CoA | Streptomyces griseus | 4-hydroxy-6-isopropyl-2-pyrone + 4-hydroxy-6-(3'-methyl-2'-oxopropyl)-2-pyrone + CoA + CO2 + H2O | - |
? | |
| isovaleryl-CoA | 13% activity compared to acetoacetyl-CoA | Streptomyces griseus | 4-hydroxy-6-isobutyl-2-pyrone + 4-hydroxy-6-(4'-methyl-2'-oxopentyl)-2-pyrone + CoA + CO2 + H2O | - |
? | |
| additional information | less than 1% activity with acetyl-CoA, decanoyl-CoA, benzoyl-CoA, phenylacetyl-CoA, crotonoyl-CoA, and tiglyl-CoA | Streptomyces griseus | ? | - |
? | |
| octanoyl-CoA | 6% activity compared to acetoacetyl-CoA | Streptomyces griseus | 6-heptyl-4-hydroxy-2-pyrone + 4-hydroxy-6-(2'-oxononyl)-2-pyrone + 4-hydroxy-6-(2',4',6'-trioxotridecyl)-2-pyrone + CoA + CO2 + H2O | - |
? | |
| octanoyl-CoA + malonyl-CoA | - |
Streptomyces griseus | 4-hydroxy-6-(2',4',6'-trioxotridecyl)-2-pyrone + CoA + CO2 + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RppA | - |
Streptomyces griseus |
| type III polyketide synthase | - |
Streptomyces griseus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Streptomyces griseus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
malonyl-CoA | in 100 mM Tris-HCl (pH 7.5), 1 mM EDTA, 1 mM 2-mercaptoethanol, at 30°C | Streptomyces griseus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Streptomyces griseus |
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