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Literature summary for 2.3.1.231 extracted from

  • Suzuki, Y.; Noma, A.; Suzuki, T.; Ishitani, R.; Nureki, O.
    Structural basis of tRNA modification with CO2 fixation and methylation by wybutosine synthesizing enzyme TYW4 (2009), Nucleic Acids Res., 37, 2910-2925.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 200 mM ammonium citrate (pH 7.0), 10 mM HEPES (pH 7.5), 20% (w/v) PEG3350, 20 mM sodium citrate, and 1% (v/v)2-propanol Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
DELTATYW4C inactive Saccharomyces cerevisiae
R88A inactive Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72430
-
1 * 72430, sedimentation velocity analytical ultracentrifugation Saccharomyces cerevisiae
80022
-
1 * 80022, calculated from amino acid sequence Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 7-[(3S)-3-amino-3-(methoxycarbonyl)propyl]wyosine72 in tRNAPhe + CO2 Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + wybutosine in tRNAPhe
-
?
S-adenosyl-L-methionine + 7-[(3S)-3-amino-3-(methoxycarbonyl)propyl]wyosine72 in tRNAPhe + CO2 Saccharomyces cerevisiae BY4742
-
S-adenosyl-L-homocysteine + wybutosine in tRNAPhe
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q08282
-
-
Saccharomyces cerevisiae BY4742 Q08282
-
-

Purification (Commentary)

Purification (Comment) Organism
Superdex 200 gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 7-[(3S)-3-amino-3-(methoxycarbonyl)propyl]wyosine72 in tRNAPhe + CO2
-
Saccharomyces cerevisiae S-adenosyl-L-homocysteine + wybutosine in tRNAPhe
-
?
S-adenosyl-L-methionine + 7-[(3S)-3-amino-3-(methoxycarbonyl)propyl]wyosine72 in tRNAPhe + CO2
-
Saccharomyces cerevisiae BY4742 S-adenosyl-L-homocysteine + wybutosine in tRNAPhe
-
?

Subunits

Subunits Comment Organism
monomer 1 * 72430, sedimentation velocity analytical ultracentrifugation Saccharomyces cerevisiae
monomer 1 * 80022, calculated from amino acid sequence Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
TYW4 formerly referred to as PPM2 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Saccharomyces cerevisiae

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the final step of wybutosine biosynthesis Saccharomyces cerevisiae