Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 200 mM ammonium citrate (pH 7.0), 10 mM HEPES (pH 7.5), 20% (w/v) PEG3350, 20 mM sodium citrate, and 1% (v/v)2-propanol | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
DELTATYW4C | inactive | Saccharomyces cerevisiae |
R88A | inactive | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72430 | - |
1 * 72430, sedimentation velocity analytical ultracentrifugation | Saccharomyces cerevisiae |
80022 | - |
1 * 80022, calculated from amino acid sequence | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 7-[(3S)-3-amino-3-(methoxycarbonyl)propyl]wyosine72 in tRNAPhe + CO2 | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + wybutosine in tRNAPhe | - |
? | |
S-adenosyl-L-methionine + 7-[(3S)-3-amino-3-(methoxycarbonyl)propyl]wyosine72 in tRNAPhe + CO2 | Saccharomyces cerevisiae BY4742 | - |
S-adenosyl-L-homocysteine + wybutosine in tRNAPhe | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q08282 | - |
- |
Saccharomyces cerevisiae BY4742 | Q08282 | - |
- |
Purification (Comment) | Organism |
---|---|
Superdex 200 gel filtration | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 7-[(3S)-3-amino-3-(methoxycarbonyl)propyl]wyosine72 in tRNAPhe + CO2 | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + wybutosine in tRNAPhe | - |
? | |
S-adenosyl-L-methionine + 7-[(3S)-3-amino-3-(methoxycarbonyl)propyl]wyosine72 in tRNAPhe + CO2 | - |
Saccharomyces cerevisiae BY4742 | S-adenosyl-L-homocysteine + wybutosine in tRNAPhe | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 72430, sedimentation velocity analytical ultracentrifugation | Saccharomyces cerevisiae |
monomer | 1 * 80022, calculated from amino acid sequence | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
TYW4 | formerly referred to as PPM2 | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the final step of wybutosine biosynthesis | Saccharomyces cerevisiae |