Literature summary for 2.3.1.23 extracted from

Jasieniecka-Gazarkiewicz, K.; Demski, K.; Lager, I.; Stymne, S.; Banas, A.
Possible role of different yeast and plant lysophospholipid acyl-CoA acyltransferases (LPLATs) in acyl remodelling of phospholipids (2016), Lipids, 51, 15-23 .

Search for more literature for 2.3.1.23

Cloned(Commentary)

Cloned (Comment)	Organism
gene AtLPCAT2, recombinant overexpression in Saccharomyces cerevisiae haploid ALE1-knockout strain (BY4742, Matalpa, his3DELTA1, leu2DELTA0, lys2DELTA0, ura3DELTA0, YOR175c::kanMX4)	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	recombinant expression of Arabidopsis thaliana LPCAT2, differences in incorporation of [14C]oleoyl-CoA into PtdCho in microsomal preparations of ale1 yeast expressing AtLPCAT2 with and without DTNB, overview	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
DTNB	complete inhibition of LPCAT in wild-type and in recombinant heterologous yeast microsomes	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Saccharomyces cerevisiae	-	-
microsome	-	Arabidopsis thaliana	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
oleoyl-CoA + 1-oleoyl-lysophosphatidic acid	Arabidopsis thaliana	-	CoA + 1,2-dioleoyl-sn-lysophosphatidic acid	-	r
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylcholine	Saccharomyces cerevisiae	-	CoA + 1,2-dioleoyl-sn-lysophosphatidylcholine	-	r
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylcholine	Arabidopsis thaliana	-	CoA + 1,2-dioleoyl-sn-lysophosphatidylcholine	-	r
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylethanolamine	Saccharomyces cerevisiae	-	CoA + 1,2-dioleoyl-sn-lysophosphatidylethanolamine	-	r
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylethanolamine	Arabidopsis thaliana	-	CoA + 1,2-dioleoyl-sn-lysophosphatidylethanolamine	-	r

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9CAN8	-	-
Saccharomyces cerevisiae	Q08548	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	substrate specificity (towards different acyl acceptors) of the lysophospholipid:acyl-CoA acyltransferase in the forward reactions with [14C]oleoyl-CoA, assay optimization. Lysophosphatidylcholine is by far the best substrate for AtLPCAT2. Ratio between reverse and forward activities for different LPLATs for different phospholipids, overview. Highest amounts in PtdCho with AtLPCAT2	Arabidopsis thaliana	?	-	-
additional information	substrate specificity (towards different acyl acceptors) of the lysophospholipid:acyl-CoA acyltransferase in the forward reactions with [14C]oleoyl-CoA, assay optimization. Ratio between reverse and forward activities for different LPLATs for different phospholipids, overview. Very high activity in the forward reaction by the Ale1, while its ability to operate reversibly on all tested lipids is very low, highest amounts in PtdCho	Saccharomyces cerevisiae	?	-	-
oleoyl-CoA + 1-oleoyl-lysophosphatidic acid	-	Arabidopsis thaliana	CoA + 1,2-dioleoyl-sn-lysophosphatidic acid	-	r
oleoyl-CoA + 1-oleoyl-lysophosphatidic acid	low activity, cf. EC 2.3.1.51	Saccharomyces cerevisiae	CoA + 1,2-dioleoyl-sn-lysophosphatidic acid	-	r
oleoyl-CoA + 1-oleoyl-lysophosphatidic acid	very low activity, cf. EC 2.3.1.51	Arabidopsis thaliana	CoA + 1,2-dioleoyl-sn-lysophosphatidic acid	-	r
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylcholine	-	Saccharomyces cerevisiae	CoA + 1,2-dioleoyl-sn-lysophosphatidylcholine	-	r
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylcholine	-	Arabidopsis thaliana	CoA + 1,2-dioleoyl-sn-lysophosphatidylcholine	-	r
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylcholine	preferred substrate, high activity	Saccharomyces cerevisiae	CoA + 1,2-dioleoyl-sn-lysophosphatidylcholine	-	r
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylcholine	preferred substrate, high activity	Arabidopsis thaliana	CoA + 1,2-dioleoyl-sn-lysophosphatidylcholine	-	r
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylethanolamine	-	Saccharomyces cerevisiae	CoA + 1,2-dioleoyl-sn-lysophosphatidylethanolamine	-	r
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylethanolamine	-	Arabidopsis thaliana	CoA + 1,2-dioleoyl-sn-lysophosphatidylethanolamine	-	r
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylethanolamine	low activity, cf. EC 2.3.1.121	Saccharomyces cerevisiae	CoA + 1,2-dioleoyl-sn-lysophosphatidylethanolamine	-	r
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylethanolamine	very low activity, cf. EC 2.3.1.121	Arabidopsis thaliana	CoA + 1,2-dioleoyl-sn-lysophosphatidylethanolamine	-	r

Synonyms

Synonyms	Comment	Organism
ALE1	-	Saccharomyces cerevisiae
AtLPLAT2	-	Arabidopsis thaliana
LPCAT2	-	Arabidopsis thaliana
LPLAT	-	Saccharomyces cerevisiae
LPLAT	-	Arabidopsis thaliana
lysophospholipid acyltransferase	-	Saccharomyces cerevisiae
lysophospholipid acyltransferase	-	Arabidopsis thaliana
lysophospholipid:acyl-CoA acyltransferase	-	Saccharomyces cerevisiae
lysophospholipid:acyl-CoA acyltransferase	-	Arabidopsis thaliana
More	see also EC 2.3.1.121 and EC 2.3.1.51	Saccharomyces cerevisiae
More	see also EC 2.3.1.121 and EC 2.3.1.51	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Saccharomyces cerevisiae
30	-	assay at	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Saccharomyces cerevisiae
7.2	-	assay at	Arabidopsis thaliana

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)