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Literature summary for 2.3.1.23 extracted from
- Lysophosphatidylethanolamine acyltransferase 2 (LPEAT2) incorporates DHA into phospholipids and has possible functions for fatty acid-induced cell death (2020), Biochem. Biophys. Res. Commun., 526, 246-252 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Agpat7, quantitative RT-PCR expression analysis, recombinant overexpression of FLAG-tagged mLPEAT2 in CHO cells | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H129A | site-directed mutagenesis, mutation of a residue in AGPAT motif I (HxxxxD), the mutant shows no LPEAT activity | Mus musculus |
| additional information | enzyme knockout in Neuro 2A cells via expression of mLPEAT2-siRNA | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | enzyme kinetics | Mus musculus | |
| 0.00314 | - |
docosahexanoyl-CoA | with 1-octadecenyl-sn-glycero-3-phosphoethanolamine, pH and temperature not specified in the publication | Mus musculus |  |
| 0.0132 | - |
docosahexanoyl-CoA | with 1-palmitoyl-sn-glycero-3-phosphoethanolamine, pH and temperature not specified in the publication | Mus musculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| docosahexaenoyl-CoA + 1-acyl-sn-glycero-3-phosphoethanolamine | Mus musculus | - |
CoA + 1-acyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q6NVG1 | enzyme complex | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipose tissue | low content | Mus musculus | - |
| brain | predominantly, high expression level of LPEAT2, expression pattern analysis, overview. mLPEAT2 is expressed in all regions, with a comparatively high expression in the cortex | Mus musculus | - |
| brain stem | - |
Mus musculus | - |
| cerebellum | - |
Mus musculus | - |
| cerebral cortex | anterior and posterior | Mus musculus | - |
| colon | low content | Mus musculus | - |
| corpus striatum | - |
Mus musculus | - |
| heart | low content | Mus musculus | - |
| hippocampus | - |
Mus musculus | - |
| hypothalamus | - |
Mus musculus | - |
| intestine | low content | Mus musculus | - |
| kidney | low content | Mus musculus | - |
| liver | low content | Mus musculus | - |
| lung | - |
Mus musculus | - |
| muscle | low content | Mus musculus | - |
| Neuro-2a cell | - |
Mus musculus | - |
| olfactory organ | - |
Mus musculus | - |
| spleen | low content | Mus musculus | - |
| stomach | higher content | Mus musculus | - |
| testis | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| docosahexaenoyl-CoA + 1-acyl-sn-glycero-3-phosphoethanolamine | - |
Mus musculus | CoA + 1-acyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine | - |
? | |
| docosahexaenoyl-CoA + 1-arachidonoyl-sn-glycero-3-phosphoethanolamine | - |
Mus musculus | CoA + 1-arachidonoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine | - |
? | |
| docosahexaenoyl-CoA + 1-oleoyl-sn-glycero-3-phosphoethanolamine | - |
Mus musculus | CoA + 1-oleoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine | - |
? | |
| docosahexaenoyl-CoA + 1-palmitoyl-sn-glycero-3-phosphoethanolamine | - |
Mus musculus | CoA + 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine | - |
? | |
| docosahexanoyl-CoA + 1-octadecenyl-sn-glycero-3-phosphoethanolamine | reaction of EC 2.3.1.25 | Mus musculus | CoA + 1-octadecenyl-2-docosahexanoyl-sn-glycero-3-phosphoethanolamine | - |
? | |
| additional information | mouse LPEAT2 (mLPEAT2) has LPEAT, LPCAT, and LPGAT activities using 22:6-CoA as a donor. mLPEAT2 shows significant activities for both acyl and alkenyl species | Mus musculus | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AGPAT7 | - |
Mus musculus |
| LPEAT2 | - |
Mus musculus |
| lysophosphatidylethanolamine acyltransferase 2 | - |
Mus musculus |
| mLPEAT2 | - |
Mus musculus |
| More | cf. EC 2.3.1.25 | Mus musculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00314 | - |
docosahexanoyl-CoA | with 1-octadecenyl-sn-glycero-3-phosphoethanolamine, pH and temperature not specified in the publication | Mus musculus | |
| 0.0132 | - |
docosahexanoyl-CoA | with 1-palmitoyl-sn-glycero-3-phosphoethanolamine, pH and temperature not specified in the publication | Mus musculus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | Neuro 2A cells overexpressing LPEAT2 underwent cell death with necrotic morphology when differentiated into neuron-like cells, with supplementation with 22:6 (DHA) | Mus musculus |
| physiological function | lysophosphatidylethanolamine acyltransferase 2 (LPEAT2), one of the enzymes that play a role in the remodeling pathway, has been reported to have lysophosphatidylcholine acyltransferase (LPCAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities with 16:0-CoA, 18:0-CoA, and 18:1-CoA as donors. LPEAT2 incorporates docosahexanoic acid (DHA) into phospholipids and has possible functions for fatty acid-induced cell death. LPEAT2 shows endogenous LPEAT activity with 22:6-CoA, and functions in modulating 22:6/20:4 ratios of phospholipids. LPEAT2 plays a role in inducing cell death DHA-dependently. Cell death induced by DHA is dependent on mLPEAT2, insights on mechanisms of neuronal necrosis | Mus musculus |
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Release 2023.1 (January 2023)
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