Literature summary for 2.3.1.226 extracted from

Hamed, R.B.; Gomez-Castellanos, J.R.; Henry, L.; Warhaut, S.; Claridge, T.D.W.; Schofield, C.J.
Biocatalytic production of bicyclic ?-lactams with three contiguous chiral centres using engineered crotonases (2019), Commun. Chem., 2, 7 .

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Application

Application	Comment	Organism
synthesis	biocatalytic production of bicyclic beta-lactams with three contiguous chiral centres using engineered crotonases. Structurally guided substitutions of wild type carboxymethylproline synthases enable tuning of their C-N and C-C bond forming capacity to produce 5-carboxymethylproline derivatives substituted at C-4 and C-6, from amino acid aldehyde and malonyl-CoA derivatives. Use of tandem enzyme incubations comprising an engineered carboxymethylproline synthase and an alkylmalonyl-CoA forming enzyme (i.e. malonyl-CoA synthetase or crotonyl-CoA carboxylase reductase) can improve stereocontrol and expand the product range. Some of the prepared 4,6-disubstituted-5-carboxymethylproline derivatives are converted to bicyclic beta-lactams by carbapenam synthetase catalysis	Pectobacterium carotovorum subsp. carotovorum

Organism

Organism	UniProt	Comment	Textmining
Pectobacterium carotovorum subsp. carotovorum	Q9XB60	-	-

Synonyms

Synonyms	Comment	Organism
CarB	-	Pectobacterium carotovorum subsp. carotovorum

General Information

General Information	Comment	Organism
metabolism	the enzyme is involved in the carbapenem biosynthesis	Pectobacterium carotovorum subsp. carotovorum

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Release 2023.1 (January 2023)