Literature summary for 2.3.1.226 extracted from

Sleeman, M.C.; Sorensen, J.L.; Batchelar, E.T.; McDonough, M.A.; Schofield, C.J.
Structural and mechanistic studies on carboxymethylproline synthase (CarB), a unique member of the crotonase superfamily catalyzing the first step in carbapenem biosynthesis (2005), J. Biol. Chem., 280, 34956-34965.

Search for more literature for 2.3.1.226

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pectobacterium carotovorum

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, crystal structure of CarB in the presence and absence of acetyl-CoA at 2.24 A and 3.15 A resolution. The structures reveal that CarB contains a conserved oxy-anion hole probably required for decarboxylation of malonyl-CoA and stabilization of the resultant enolate	Pectobacterium carotovorum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
malonyl-CoA + (S)-1-pyrroline-5-carboxylate + H2O	Pectobacterium carotovorum	-	CoA + (2S,5S)-5-carboxymethylproline + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Pectobacterium carotovorum	Q9XB60	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
malonyl-CoA + (S)-1-pyrroline-5-carboxylate + H2O	-	Pectobacterium carotovorum	CoA + (2S,5S)-5-carboxymethylproline + CO2	-	?

Synonyms

Synonyms	Comment	Organism
CarB	-	Pectobacterium carotovorum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Pectobacterium carotovorum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Pectobacterium carotovorum

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Release 2023.1 (January 2023)