Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pectobacterium carotovorum |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, crystal structure of CarB in the presence and absence of acetyl-CoA at 2.24 A and 3.15 A resolution. The structures reveal that CarB contains a conserved oxy-anion hole probably required for decarboxylation of malonyl-CoA and stabilization of the resultant enolate | Pectobacterium carotovorum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + (S)-1-pyrroline-5-carboxylate + H2O | Pectobacterium carotovorum | - |
CoA + (2S,5S)-5-carboxymethylproline + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pectobacterium carotovorum | Q9XB60 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + (S)-1-pyrroline-5-carboxylate + H2O | - |
Pectobacterium carotovorum | CoA + (2S,5S)-5-carboxymethylproline + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CarB | - |
Pectobacterium carotovorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pectobacterium carotovorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pectobacterium carotovorum |