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Literature summary for 2.3.1.225 extracted from

  • Dunphy, J.T.; Schroeder, H.; Leventis, R.; Greentree, W.K; Knudsen, J.K.; Silvius, J.R.; Linder, M.E.
    Differential effects of acyl-CoA binding protein on enzymatic and non-enzymatic thioacylation of protein and peptide substrates (2000), Biochim. Biophys. Acta, 1485, 185-198.
    View publication on PubMed
Search for more literature for 2.3.1.225

Activating Compound

Activating Compound Comment Organism Structure
acyl-CoA binding protein i.e. ACBP strongly suppress non-enzymatic thioacylation of cysteinyl containing peptides by long-chain acyl-CoAs, usage of purified rat and recombinant bovine liver ACBP. ACBP only modestly inhibits enzymatic thioacylation of a myristoylated peptide or G-protein K-subunits under conditions where non-enzymatic thioacylation is reduced to background Bos taurus
acyl-CoA binding protein i.e. ACBP strongly suppress non-enzymatic thioacylation of cysteinyl containing peptides by long-chain acyl-CoAs, usage of purified rat and recombinant bovine liver ACBP. ACBP only modestly inhibits enzymatic thioacylation of a myristoylated peptide or G-protein K-subunits under conditions where non-enzymatic thioacylation is reduced to background Chlorocebus aethiops

Inhibitors

Inhibitors Comment Organism Structure
acyl-CoA binding protein i.e. ACBP strongly suppress non-enzymatic thioacylation of cysteinyl containing peptides by long-chain acyl-CoAs, usage of purified rat and recombinant bovine liver ACBP. ACBP only modestly inhibits enzymatic thioacylation of a myristoylated peptide or G-protein K-subunits under conditions where non-enzymatic thioacylation is reduced to background Bos taurus
acyl-CoA binding protein i.e. ACBP strongly suppress non-enzymatic thioacylation of cysteinyl containing peptides by long-chain acyl-CoAs, usage of purified rat and recombinant bovine liver ACBP. ACBP only modestly inhibits enzymatic thioacylation of a myristoylated peptide or G-protein K-subunits under conditions where non-enzymatic thioacylation is reduced to background Chlorocebus aethiops

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Bos taurus 5783
-
endoplasmic reticulum
-
 Chlorocebus aethiops 5783
-
Golgi membrane
-
 Bos taurus 139
-
Golgi membrane
-
 Chlorocebus aethiops 139
-
membrane
-
 Bos taurus 16020
-
membrane
-
 Chlorocebus aethiops 16020
-
plasma membrane
-
 Bos taurus 5886
-
plasma membrane
-
 Chlorocebus aethiops 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
palmitoyl-CoA + [G protein alpha subunit]-L-cysteine Bos taurus
-
 [G protein alpha subunit]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [G protein alpha subunit]-L-cysteine Chlorocebus aethiops
-
 [G protein alpha subunit]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [protein]-L-cysteine Bos taurus
-
 [protein]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [protein]-L-cysteine Chlorocebus aethiops
-
 [protein]-S-palmitoyl-L-cysteine + CoA
-
 r

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Chlorocebus aethiops
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 120fold by anion exchange and hydroxylapatite chromatography Bos taurus
native enzyme 120fold by anion exchange and hydroxylapatite chromatography Chlorocebus aethiops

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Bos taurus
-
CV-1 cell
-
 Chlorocebus aethiops
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PAT activity displays specificity for the acyl donor, efficiently utilizing long-chain acyl-CoAs, but not free fatty acid or S-palmitoyl-N-acetylcysteamine. PAT also shows thiolase activity Bos taurus ?
-
 ?
additional information PAT activity displays specificity for the acyl donor, efficiently utilizing long-chain acyl-CoAs, but not free fatty acid or S-palmitoyl-N-acetylcysteamine. PAT also shows thiolase activity Chlorocebus aethiops ?
-
 ?
palmitoyl-CoA + [G protein alpha subunit Gialpha1]-L-cysteine purified recombinant Gialpha1 Bos taurus [G protein alpha subunit Gialpha1]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [G protein alpha subunit Gialpha1]-L-cysteine purified recombinant Gialpha1 Chlorocebus aethiops [G protein alpha subunit Gialpha1]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [G protein alpha subunit]-L-cysteine
-
 Bos taurus [G protein alpha subunit]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [G protein alpha subunit]-L-cysteine
-
 Chlorocebus aethiops [G protein alpha subunit]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [protein]-L-cysteine
-
 Bos taurus [protein]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [protein]-L-cysteine
-
 Chlorocebus aethiops [protein]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [short lipid-modified cysteinyl-containing peptide]-L-cysteine minimum requirements for substrate recognition are a free cysteine thiol adjacent to a hydrophobic lipid anchor, either myristate or farnesyl isoprenoid, overview Bos taurus [short lipid-modified cysteinyl-containing peptide]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [short lipid-modified cysteinyl-containing peptide]-L-cysteine minimum requirements for substrate recognition are a free cysteine thiol adjacent to a hydrophobic lipid anchor, either myristate or farnesyl isoprenoid, overview Chlorocebus aethiops [short lipid-modified cysteinyl-containing peptide]-S-palmitoyl-L-cysteine + CoA
-
 r

Synonyms

Synonyms Comment Organism
Pat
-
 Bos taurus
Pat
-
 Chlorocebus aethiops

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 thiolase assay at room temperature Bos taurus
22
-
 thiolase assay at room temperature Chlorocebus aethiops
30 37 assay at Bos taurus
30 37 assay at Chlorocebus aethiops

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4 8 assay at Bos taurus
6.4 8 assay at Chlorocebus aethiops
8.3
-
 thiolase assay at Bos taurus
8.3
-
 thiolase assay at Chlorocebus aethiops

General Information

General Information Comment Organism
physiological function reversible modification by palmitate is a feature of many signaling proteins that are associated with the cytoplasmic leaflet of the plasma membrane Bos taurus
physiological function reversible modification by palmitate is a feature of many signaling proteins that are associated with the cytoplasmic leaflet of the plasma membrane Chlorocebus aethiops