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Literature summary for 2.3.1.224 extracted from

  • Suzuki, H.; Sawada, S.; Watanabe, K.; Nagae, S.; Yamaguchi, M.A.; Nakayama, T.; Nishino, T.
    Identification and characterization of a novel anthocyanin malonyltransferase from scarlet sage (Salvia splendens) flowers: an enzyme that is phylogenetically separated from other anthocyanin acyltransferases (2004), Plant J., 38, 994-1003.
    View publication on PubMed
Search for more literature for 2.3.1.224

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Salvia splendens

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA 32% residual activity at 0.1 mM Salvia splendens
bisdemalonylsalvianin 31% residual activity at 0.05 mM Salvia splendens
caffeic acid 38% residual activity at 1 mM Salvia splendens
Cd2+ complete inhibition at 0.1 mM Salvia splendens
CoA-SH complete inhibition at 0.1 mM Salvia splendens
Cu2+ complete inhibition at 0.1 mM Salvia splendens
diethyldicarbonate 69% residual activity at 0.1 mM Salvia splendens
Fe2+ complete inhibition at 0.1 mM Salvia splendens
Hg+ complete inhibition at 0.1 mM Salvia splendens
N-ethylmaleimide complete inhibition at 5 mM Salvia splendens
Zn2+ complete inhibition at 0.1 mM Salvia splendens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0046
-
 malonyl-CoA recombinant enzyme, at pH 7.0 and 30°C Salvia splendens
0.0069
-
 malonyl-CoA native enzyme, at pH 7.0 and 30°C Salvia splendens
0.0513
-
 monodemalonylsalvianin native enzyme, at pH 7.0 and 30°C Salvia splendens
0.0563
-
 monodemalonylsalvianin recombinant enzyme, at pH 7.0 and 30°C Salvia splendens

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not influenced by Ca2+, Co2+, Mg2+, Mn2+, Ni2+, and Sn2+ Salvia splendens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 gel filtration Salvia splendens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonyl-CoA + cyanidin 3-O-(6''-O-4-coumarylglucoside)-5-O-(6'''-O-malonylglucoside) Salvia splendens
-
 CoA + ?
-
 ?
malonyl-CoA + monodemalonylsalvianin Salvia splendens
-
 CoA + salvianin
-
 ?

Organism

Organism UniProt Comment Textmining
Salvia splendens Q6TXD2
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, DEAE-Sepharose column chromatography, phenyl Sepharose column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration Salvia splendens

Source Tissue

Source Tissue Comment Organism Textmining
flower highest expression Salvia splendens
-
root
-
 Salvia splendens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000047
-
 crude extract, at pH 7.0 and 30°C Salvia splendens
0.0024
-
 after 510fold purification, at pH 7.0 and 30°C Salvia splendens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + cyanidin 3-O-(6''-O-4-coumarylglucoside)-5-O-(6'''-O-malonylglucoside)
-
 Salvia splendens CoA + ?
-
 ?
malonyl-CoA + monodemalonylsalvianin
-
 Salvia splendens CoA + salvianin
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 46000, SDS-PAGE Salvia splendens

Synonyms

Synonyms Comment Organism
5MaT2
-
 Salvia splendens
anthocyanin malonyltransferase
-
 Salvia splendens
malonyl-CoA:anthocyanin 5-glucoside 4'''-O-malonyltransferase
-
 Salvia splendens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.55
-
 malonyl-CoA native enzyme, at pH 7.0 and 30°C Salvia splendens
0.55
-
 monodemalonylsalvianin native enzyme, at pH 7.0 and 30°C Salvia splendens
0.76
-
 malonyl-CoA recombinant enzyme, at pH 7.0 and 30°C Salvia splendens
0.76
-
 monodemalonylsalvianin recombinant enzyme, at pH 7.0 and 30°C Salvia splendens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10.7
-
 monodemalonylsalvianin native enzyme, at pH 7.0 and 30°C Salvia splendens
13.5
-
 monodemalonylsalvianin recombinant enzyme, at pH 7.0 and 30°C Salvia splendens
79.7
-
 malonyl-CoA native enzyme, at pH 7.0 and 30°C Salvia splendens
165
-
 malonyl-CoA recombinant enzyme, at pH 7.0 and 30°C Salvia splendens