Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Salvia splendens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | 32% residual activity at 0.1 mM | Salvia splendens | |
bisdemalonylsalvianin | 31% residual activity at 0.05 mM | Salvia splendens | |
caffeic acid | 38% residual activity at 1 mM | Salvia splendens | |
Cd2+ | complete inhibition at 0.1 mM | Salvia splendens | |
CoA-SH | complete inhibition at 0.1 mM | Salvia splendens | |
Cu2+ | complete inhibition at 0.1 mM | Salvia splendens | |
diethyldicarbonate | 69% residual activity at 0.1 mM | Salvia splendens | |
Fe2+ | complete inhibition at 0.1 mM | Salvia splendens | |
Hg+ | complete inhibition at 0.1 mM | Salvia splendens | |
N-ethylmaleimide | complete inhibition at 5 mM | Salvia splendens | |
Zn2+ | complete inhibition at 0.1 mM | Salvia splendens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0046 | - |
malonyl-CoA | recombinant enzyme, at pH 7.0 and 30°C | Salvia splendens | |
0.0069 | - |
malonyl-CoA | native enzyme, at pH 7.0 and 30°C | Salvia splendens | |
0.0513 | - |
monodemalonylsalvianin | native enzyme, at pH 7.0 and 30°C | Salvia splendens | |
0.0563 | - |
monodemalonylsalvianin | recombinant enzyme, at pH 7.0 and 30°C | Salvia splendens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not influenced by Ca2+, Co2+, Mg2+, Mn2+, Ni2+, and Sn2+ | Salvia splendens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
gel filtration | Salvia splendens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + cyanidin 3-O-(6''-O-4-coumarylglucoside)-5-O-(6'''-O-malonylglucoside) | Salvia splendens | - |
CoA + ? | - |
? | |
malonyl-CoA + monodemalonylsalvianin | Salvia splendens | - |
CoA + salvianin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salvia splendens | Q6TXD2 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, DEAE-Sepharose column chromatography, phenyl Sepharose column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration | Salvia splendens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | highest expression | Salvia splendens | - |
root | - |
Salvia splendens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000047 | - |
crude extract, at pH 7.0 and 30°C | Salvia splendens |
0.0024 | - |
after 510fold purification, at pH 7.0 and 30°C | Salvia splendens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + cyanidin 3-O-(6''-O-4-coumarylglucoside)-5-O-(6'''-O-malonylglucoside) | - |
Salvia splendens | CoA + ? | - |
? | |
malonyl-CoA + monodemalonylsalvianin | - |
Salvia splendens | CoA + salvianin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 46000, SDS-PAGE | Salvia splendens |
Synonyms | Comment | Organism |
---|---|---|
5MaT2 | - |
Salvia splendens |
anthocyanin malonyltransferase | - |
Salvia splendens |
malonyl-CoA:anthocyanin 5-glucoside 4'''-O-malonyltransferase | - |
Salvia splendens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.55 | - |
malonyl-CoA | native enzyme, at pH 7.0 and 30°C | Salvia splendens | |
0.55 | - |
monodemalonylsalvianin | native enzyme, at pH 7.0 and 30°C | Salvia splendens | |
0.76 | - |
malonyl-CoA | recombinant enzyme, at pH 7.0 and 30°C | Salvia splendens | |
0.76 | - |
monodemalonylsalvianin | recombinant enzyme, at pH 7.0 and 30°C | Salvia splendens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.7 | - |
monodemalonylsalvianin | native enzyme, at pH 7.0 and 30°C | Salvia splendens | |
13.5 | - |
monodemalonylsalvianin | recombinant enzyme, at pH 7.0 and 30°C | Salvia splendens | |
79.7 | - |
malonyl-CoA | native enzyme, at pH 7.0 and 30°C | Salvia splendens | |
165 | - |
malonyl-CoA | recombinant enzyme, at pH 7.0 and 30°C | Salvia splendens |