Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Rhodopseudomonas palustris |
expression in Escherichia coli BL21(DE3) | Salmonella enterica subsp. enterica serovar Typhimurium |
Crystallization (Comment) | Organism |
---|---|
crystals are obtained from sitting drop experiments at 22°C. High-resolution PduL crystal structures with bound substrates. The PduL fold is unrelated to that of Pta. It contains a dimetal active site involved in a catalytic mechanism distinct from that of the housekeeping phosphotransacylase (PTAC) | Rhodopseudomonas palustris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodopseudomonas palustris | Q21A54 | - |
- |
Rhodopseudomonas palustris BisB18 | Q21A54 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | Q9XDN5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodopseudomonas palustris |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
propanoyl-CoA + phosphate | - |
Rhodopseudomonas palustris | CoA + propanoyl phosphate | - |
? | |
propanoyl-CoA + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CoA + propanoyl phosphate | - |
? | |
propanoyl-CoA + phosphate | - |
Rhodopseudomonas palustris BisB18 | CoA + propanoyl phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PduL | - |
Rhodopseudomonas palustris |
PduL | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
General Information | Comment | Organism |
---|---|---|
evolution | the phosphotransacylase (PTAC) predominantly associated with metabolosomes (PduL) has no sequence homology to the PTAC ubiquitous among fermentative bacteria (Pta). PduL and Pta exemplify functional, but not structural, convergent evolution | Rhodopseudomonas palustris |
evolution | the phosphotransacylase (PTAC) predominantly associated with metabolosomes (PduL) has no sequence homology to the PTAC ubiquitous among fermentative bacteria (Pta). PduL and Pta exemplify functional, but not structural, convergent evolution | Salmonella enterica subsp. enterica serovar Typhimurium |
metabolism | the fact that PduL is confined almost exclusively to metabolosomes can be used to develop an inhibitor that blocks only PduL and not Pta as a way to selectively disrupt bacterial microcompartments(BMC)-based metabolism, while not affecting most commensal organisms that require phosphotransacylase (PTAC) activity | Rhodopseudomonas palustris |
metabolism | the fact that PduL is confined almost exclusively to metabolosomes can be used to develop an inhibitor that blocks only PduL and not Pta as a way to selectively disrupt bacterial microcompartments(BMC)-based metabolism, while not affecting most commensal organisms that require phosphotransacylase (PTAC) activity | Salmonella enterica subsp. enterica serovar Typhimurium |