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Literature summary for 2.3.1.222 extracted from
- Two propanediol utilization-like proteins of Moorella thermoacetica with phosphotransacetylase activity (2016), Extremophiles, 20, 653-661 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Moorella thermoacetica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 0.1 microM, 82% residual activity; 0.2 microM, 40% residual activity | Moorella thermoacetica |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
butyryl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 0.02 | - |
butanoyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 0.04 | - |
acetyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 0.04 | - |
propionyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 0.04 | - |
propanoyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 0.12 | - |
propionyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 0.12 | - |
propanoyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 0.4 | - |
acetyl phosphate | pH 7.4, 55°C | Moorella thermoacetica | |
| 0.49 | - |
acetyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 0.0003 mM, 29% inhibition | Moorella thermoacetica | |
| Mn2+ | 0.0003 mM, 84% inhibition | Moorella thermoacetica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
SDS-PAGE | Moorella thermoacetica |
| 90000 | - |
gel filtration | Moorella thermoacetica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Moorella thermoacetica | Q2RJ94 | - |
- |
| Moorella thermoacetica | Q2RJ94 | isoform Pdul1 | - |
| Moorella thermoacetica | Q2RK58 | - |
- |
| Moorella thermoacetica | Q2RK58 | isoform Pdul2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Moorella thermoacetica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + phosphate | - |
Moorella thermoacetica | CoA + acetyl phosphate | - |
r | |
| acetyl-CoA + phosphate | no activity with acetyl phosphate in the reverse direction | Moorella thermoacetica | CoA + acetyl phosphate | - |
ir | |
| acetyl-CoA + phosphate | reaction of EC 2.3.1.8 | Moorella thermoacetica | CoA + acetyl phosphate | - |
ir | |
| acetyl-CoA + phosphate | reaction of EC 2.3.1.8 | Moorella thermoacetica | CoA + acetyl phosphate | - |
r | |
| butanoyl-CoA + phosphate | reaction of EC 2.3.1.19 | Moorella thermoacetica | CoA + butanoyl phosphate | - |
? | |
| butyryl-CoA + phosphate | - |
Moorella thermoacetica | CoA + butyryl phosphate | - |
r | |
| CoA + acetyl phosphate | - |
Moorella thermoacetica | acetyl-CoA + phosphate | - |
r | |
| CoA + butyryl phosphate | - |
Moorella thermoacetica | butyryl-CoA + phosphate | - |
r | |
| CoA + propanoyl phosphate | - |
Moorella thermoacetica | propanoyl-CoA + phosphate | - |
r | |
| additional information | no activity with butyryl phosphate | Moorella thermoacetica | ? | - |
- |
|
| additional information | no substrates: butanoyl-CoA, acetyl phosphate | Moorella thermoacetica | ? | - |
- |
|
| propanoyl-CoA + phosphate | - |
Moorella thermoacetica | CoA + propanoyl phosphate | - |
r | |
| propionyl-CoA + phosphate | - |
Moorella thermoacetica | CoA + propionyl phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 23000, SDS-PAGE | Moorella thermoacetica |
| tetramer | 4 * 23000, SDS-PAGE | Moorella thermoacetica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Moth_0864 | - |
Moorella thermoacetica |
| Moth_1181 | - |
Moorella thermoacetica |
| pduL1 | - |
Moorella thermoacetica |
| pduL2 | - |
Moorella thermoacetica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Moorella thermoacetica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
butyryl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 0.12 | - |
butanoyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 0.2 | - |
acetyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 0.21 | - |
propionyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 0.21 | - |
propanoyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 1.14 | - |
propionyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 1.14 | - |
propanoyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 2.65 | - |
acetyl phosphate | pH 7.4, 55°C | Moorella thermoacetica | |
| 5.18 | - |
acetyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Moorella thermoacetica |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.67 | - |
acetyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 4.69 | - |
acetyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 5.03 | - |
propionyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 5.03 | - |
propanoyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 6.56 | - |
acetyl phosphate | pH 7.4, 55°C | Moorella thermoacetica | |
| 6.68 | - |
butyryl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 6.68 | - |
butanoyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 9.26 | - |
propionyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 9.26 | - |
propanoyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
| 10.67 | - |
acetyl-CoA | pH 7.6, 55°C | Moorella thermoacetica | |
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Release 2023.1 (January 2023)
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