BRENDA - Enzyme Database show
show all sequences of 2.3.1.221

The Aspergillus parasiticus polyketide synthase gene pksA, a homolog of Aspergillus nidulans wA, is required for aflatoxin B1 biosynthesis

Chang, P.K.; Cary, J.W.; Yu, J.; Bhatnagar, D.; Cleveland, T.E.; Mol. Gen. Genet. 248, 270-277 (1995)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene pksA, DNA and amino acid sequence determination and analysis
Aspergillus parasiticus
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of strain SRRC 2043, a disruption mutant defective in the pksA locus
Aspergillus parasiticus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus parasiticus
Q12053
gene pksA
-
Aspergillus parasiticus SRRC 2043 / RHN1
Q12053
gene pksA
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus parasiticus
-
Cloned(Commentary) (protein specific)
Commentary
Organism
gene pksA, DNA and amino acid sequence determination and analysis
Aspergillus parasiticus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of strain SRRC 2043, a disruption mutant defective in the pksA locus
Aspergillus parasiticus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus parasiticus
-
General Information
General Information
Commentary
Organism
evolution
the pksA gene is located in the aflatoxin pathway gene cluster and is linked to the nor-1 gene, an aflatoxin pathway gene required for converting norsolorinic acid to averantin
Aspergillus parasiticus
malfunction
strain SRRC 2043 is a disruption mutant defective in the pksA locus. Disruption of pksA results in an inability to produce aflatoxin intermediates
Aspergillus parasiticus
metabolism
pksA is a polyketide synthase gene required for the early steps of aflatoxin biosynthesis
Aspergillus parasiticus
General Information (protein specific)
General Information
Commentary
Organism
evolution
the pksA gene is located in the aflatoxin pathway gene cluster and is linked to the nor-1 gene, an aflatoxin pathway gene required for converting norsolorinic acid to averantin
Aspergillus parasiticus
malfunction
strain SRRC 2043 is a disruption mutant defective in the pksA locus. Disruption of pksA results in an inability to produce aflatoxin intermediates
Aspergillus parasiticus
metabolism
pksA is a polyketide synthase gene required for the early steps of aflatoxin biosynthesis
Aspergillus parasiticus
Other publictions for EC 2.3.1.221
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722459
Vagstad
Interrogation of global active ...
Aspergillus parasiticus
J. Am. Chem. Soc.
134
6865-6877
2012
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1
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2
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2
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723638
Korman
Structure and function of an i ...
Aspergillus sp.
Proc. Natl. Acad. Sci. USA
107
6246-6251
2010
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1
4
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2
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723262
Crawford
Structural basis for biosynthe ...
Aspergillus parasiticus
Nature
461
1139-1143
2009
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1
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721052
Crawford
Deconstruction of iterative mu ...
Aspergillus parasiticus
Science
320
243-246
2008
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1
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1
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2
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723194
Chang
The Aspergillus parasiticus po ...
Aspergillus parasiticus, Aspergillus parasiticus SRRC 2043 / RHN1
Mol. Gen. Genet.
248
270-277
1995
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3
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