BRENDA - Enzyme Database
show all sequences of 2.3.1.221

Deconstruction of iterative multidomain polyketide synthase function

Crawford, J.M.; Thomas, P.M.; Scheerer, J.R.; Vagstad, A.L.; Kelleher, N.L.; Townsend, C.A.; Science 320, 243-246 (2008)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene pksA, cloning of various mono-, di-, and tridomains for combinatorial reconstitution experiments, expression of the either C- or N-terminally His6-tagged protein fragments in Escherichia coli
Aspergillus parasiticus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
7 malonyl-CoA + hexanoyl-[acyl-carrier protein]
Aspergillus parasiticus
-
7 CoA + norsolorinic acid anthrone + [acyl-carrier protein] + 7 CO2 + 2 H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Aspergillus parasiticus
-
; gene pksA
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant C- or N-terminally His6-tagged mono-, di-, and tridomains protein fragments from Escherichia coli by metal affinity cromatography
Aspergillus parasiticus
Reaction
Reaction
Commentary
Organism
Reaction ID
7 malonyl-CoA + hexanoyl-[acyl-carrier protein] = 7 CoA + norsolorinic acid anthrone + [acyl-carrier protein] + 7 CO2 + 2 H2O
a product template domain unites with the ketosynthase and thioesterase in this IPKS system to assemble precisely seven malonyl-derived building blocks to a hexanoyl starter unit and mediate a specific cyclization cascade. These mechanistic features are general for IPKS-catalyzed production of aromatic polyketides
Aspergillus parasiticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
7 malonyl-CoA + hexanoyl-[acyl-carrier protein]
-
721052
Aspergillus parasiticus
7 CoA + norsolorinic acid anthrone + [acyl-carrier protein] + 7 CO2 + 2 H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
domain structure, and dissection of domains, interactions, overview
Aspergillus parasiticus
Synonyms
Synonyms
Commentary
Organism
norsolorinic acid anthrone synthase
-
Aspergillus parasiticus
PksA
-
Aspergillus parasiticus
polyketide synthase A
-
Aspergillus parasiticus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene pksA, cloning of various mono-, di-, and tridomains for combinatorial reconstitution experiments, expression of the either C- or N-terminally His6-tagged protein fragments in Escherichia coli
Aspergillus parasiticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
7 malonyl-CoA + hexanoyl-[acyl-carrier protein]
Aspergillus parasiticus
-
7 CoA + norsolorinic acid anthrone + [acyl-carrier protein] + 7 CO2 + 2 H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant C- or N-terminally His6-tagged mono-, di-, and tridomains protein fragments from Escherichia coli by metal affinity cromatography
Aspergillus parasiticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
7 malonyl-CoA + hexanoyl-[acyl-carrier protein]
-
721052
Aspergillus parasiticus
7 CoA + norsolorinic acid anthrone + [acyl-carrier protein] + 7 CO2 + 2 H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
domain structure, and dissection of domains, interactions, overview
Aspergillus parasiticus
General Information
General Information
Commentary
Organism
metabolism
PksA is involved in the synthesis of norsolorinic acid, an intermediate in the biosynthesis of the environmental carcinogen aflatoxin B1
Aspergillus parasiticus
physiological function
PksA, which initiates biosynthesis of the environmental carcinogen aflatoxin B1, is one of the multidomain iterative polyketide synthases, IPKSs
Aspergillus parasiticus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
PksA is involved in the synthesis of norsolorinic acid, an intermediate in the biosynthesis of the environmental carcinogen aflatoxin B1
Aspergillus parasiticus
physiological function
PksA, which initiates biosynthesis of the environmental carcinogen aflatoxin B1, is one of the multidomain iterative polyketide synthases, IPKSs
Aspergillus parasiticus
Other publictions for EC 2.3.1.221
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722459
Vagstad
Interrogation of global active ...
Aspergillus parasiticus
J. Am. Chem. Soc.
134
6865-6877
2012
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1
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1
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2
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1
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1
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1
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2
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2
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723638
Korman
Structure and function of an i ...
Aspergillus sp.
Proc. Natl. Acad. Sci. USA
107
6246-6251
2010
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1
1
4
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1
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1
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1
1
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1
1
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1
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1
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1
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1
4
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1
1
1
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1
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2
2
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723262
Crawford
Structural basis for biosynthe ...
Aspergillus parasiticus
Nature
461
1139-1143
2009
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1
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1
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1
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1
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1
1
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1
1
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721052
Crawford
Deconstruction of iterative mu ...
Aspergillus parasiticus
Science
320
243-246
2008
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1
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1
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1
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1
1
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1
1
4
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1
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1
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1
1
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2
2
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723194
Chang
The Aspergillus parasiticus po ...
Aspergillus parasiticus, Aspergillus parasiticus SRRC 2043 / RHN1
Mol. Gen. Genet.
248
270-277
1995
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1
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1
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1
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1
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1
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3
3
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