Literature summary for 2.3.1.221 extracted from

Crawford, J.M.; Thomas, P.M.; Scheerer, J.R.; Vagstad, A.L.; Kelleher, N.L.; Townsend, C.A.
Deconstruction of iterative multidomain polyketide synthase function (2008), Science, 320, 243-246.

Search for more literature for 2.3.1.221

Cloned(Commentary)

Cloned (Comment)	Organism
gene pksA, cloning of various mono-, di-, and tridomains for combinatorial reconstitution experiments, expression of the either C- or N-terminally His6-tagged protein fragments in Escherichia coli	Aspergillus parasiticus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
7 malonyl-CoA + hexanoyl-[acyl-carrier protein]	Aspergillus parasiticus	-	7 CoA + norsolorinic acid anthrone + [acyl-carrier protein] + 7 CO2 + 2 H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Aspergillus parasiticus	-	-	-
Aspergillus parasiticus	-	gene pksA	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant C- or N-terminally His6-tagged mono-, di-, and tridomains protein fragments from Escherichia coli by metal affinity cromatography	Aspergillus parasiticus

Reaction

Reaction	Comment	Organism	Reaction ID
7 malonyl-CoA + hexanoyl-[acyl-carrier protein] = 7 CoA + norsolorinic acid anthrone + [acyl-carrier protein] + 7 CO2 + 2 H2O	a product template domain unites with the ketosynthase and thioesterase in this IPKS system to assemble precisely seven malonyl-derived building blocks to a hexanoyl starter unit and mediate a specific cyclization cascade. These mechanistic features are general for IPKS-catalyzed production of aromatic polyketides	Aspergillus parasiticus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7 malonyl-CoA + hexanoyl-[acyl-carrier protein]	-	Aspergillus parasiticus	7 CoA + norsolorinic acid anthrone + [acyl-carrier protein] + 7 CO2 + 2 H2O	-	?

Subunits

Subunits	Comment	Organism
More	domain structure, and dissection of domains, interactions, overview	Aspergillus parasiticus

Synonyms

Synonyms	Comment	Organism
norsolorinic acid anthrone synthase	-	Aspergillus parasiticus
PksA	-	Aspergillus parasiticus
polyketide synthase A	-	Aspergillus parasiticus

General Information

General Information	Comment	Organism
metabolism	PksA is involved in the synthesis of norsolorinic acid, an intermediate in the biosynthesis of the environmental carcinogen aflatoxin B1	Aspergillus parasiticus
physiological function	PksA, which initiates biosynthesis of the environmental carcinogen aflatoxin B1, is one of the multidomain iterative polyketide synthases, IPKSs	Aspergillus parasiticus

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Release 2023.1 (January 2023)