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Literature summary for 2.3.1.219 extracted from

  • Zhang, L.; Gao, B.; Wang, X.; Zhang, Z.; Liu, X.; Wang, J.; Mo, T.; Liu, Y.; Shi, S.; Tu, P.
    Identification of a new curcumin synthase from ginger and construction of a curcuminoid-producing unnatural fusion protein diketide-CoA synthase curcumin synthase (2016), RSC Adv., 6, 12519-12524 .
No PubMed abstract available
Search for more literature for 2.3.1.219

Application

Application Comment Organism
synthesis a curcuminoid producing unnatural fusion protein diketide-CoA synthase:curcumin synthase is constructed. The fusion protein may contribute to further understand the biosynthesis of curcuminoids in ginger but also be advantage to further manipulate the biosynthesis of curcuminoid analogs, particularly including tetrahydrobisdemethoxycurcumin (THBDC) and various dihydrocurcuminoid derivatives in microorganisms Zingiber officinale

Cloned(Commentary)

Cloned (Comment) Organism
diketide-CoA synthase, curcumin synthase, and the unnatural fusion protein diketide-CoA synthase:CURS His-tagged at their N terminus are produced in Escherichia coli BL21 (DE3) Zingiber officinale

Protein Variants

Protein Variants Comment Organism
additional information a curcuminoid producing unnatural fusion protein diketide-CoA synthase:curcumin synthase is constructed Zingiber officinale

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43240
-
 SDS-PAGE Zingiber officinale
85470
-
 diketide-CoA synthase:CURS His-tagged at their N terminus are produced in Escherichia coli BL21 (DE3), SDS-PAGE Zingiber officinale

Organism

Organism UniProt Comment Textmining
Zingiber officinale A5GZV8
-
-

Purification (Commentary)

Purification (Comment) Organism
diketide-CoA synthase, curcumin synthase, and the unnatural fusion protein diketide-CoA synthase:curcumin synthase His-tagged at their N terminus are produced in Escherichia coli BL21 (DE3) Zingiber officinale

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + (4-coumaroyl)acetyl-CoA + H2O curcumin synthase (ZoCURS), in the presence of diketide-CoA synthase, can accept p-coumaroyl-CoA to produce bidemethoxycurcumin Zingiber officinale 2 CoA + bidemethoxycurcumin + CO2
-
 ?
cinnamoyl-CoA + cinnamoyl-acetyl-CoA + H2O curcumin synthase (ZoCURS), in the presence of diketide-CoA synthase, can accept cinnamoyl-CoA to produce dicinnamoylmethane Zingiber officinale 2 CoA + dicinnamoylmethane + CO2
-
 ?
additional information when diketide-CoA synthase (DCS) and curcumin synthase (ZoCURS) are co-incubated in the presence of 3-(4-hydroxyphenyl)propionyl-CoA and malonyl-CoA, tetrahydrobisdemethoxycurcumin and 4-hydroxybenzylacetone are produced Zingiber officinale ?
-
-

Synonyms

Synonyms Comment Organism
ZoCURS
-
 Zingiber officinale