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Literature summary for 2.3.1.218 extracted from

  • Katsuyama, Y.; Kita, T.; Funa, N.; Horinouchi, S.
    Curcuminoid biosynthesis by two type III polyketide synthases in the herb Curcuma longa (2009), J. Biol. Chem., 284, 11160-11170.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression analysis by quantitative real-time PCR, recombinant expression as N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Curcuma longa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information DCS is an allosteric enzyme. Homotropic activation decreases the activity as the substrate concentration decreases more quickly than those exhibiting Michaelis-Menten-type kinetics. Kinetic analysis of DCS, overview Curcuma longa
0.0084
-
malonyl-CoA pH 7.5, 37°C, recombinant enzyme Curcuma longa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-coumaroyl-CoA + malonyl-CoA Curcuma longa
-
(4-coumaroyl)acetyl-CoA + CO2 + CoA
-
?
feruloyl-CoA + malonyl-CoA Curcuma longa
-
feruloylacetyl-CoA + CO2 + CoA
-
?

Organism

Organism UniProt Comment Textmining
Curcuma longa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Curcuma longa
-
rhizome
-
Curcuma longa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + malonyl-CoA
-
Curcuma longa (4-coumaroyl)acetyl-CoA + CO2 + CoA
-
?
feruloyl-CoA + malonyl-CoA
-
Curcuma longa feruloylacetyl-CoA + CO2 + CoA
-
?
feruloyl-CoA + malonyl-CoA
-
Curcuma longa feruloylacetyl-CoA + CO2 + CoA product identification by LC-ESI MS/MS ?

Synonyms

Synonyms Comment Organism
DCS
-
Curcuma longa
diketide-CoA synthase
-
Curcuma longa
phenylpropanoyl-diketide-CoA synthase
-
Curcuma longa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Curcuma longa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0112
-
malonyl-CoA pH 7.5, 37°C, recombinant enzyme Curcuma longa
0.02
-
feruloyl-CoA pH 7.5, 37°C, recombinant enzyme Curcuma longa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Curcuma longa

General Information

General Information Comment Organism
evolution the enzyme belongs to the Type III polyketide synthase family, but DCS is a unique type III PKS that releases the product as a CoA-bound form Curcuma longa
metabolism the enzyme catalyzes a step in the curcuminoid biosynthesis, pathway overview Curcuma longa
additional information DCS can collaborate with curcumin synthase, CURS, EC 2.3.1.217, which uses the reaction product feruloylacetyl-CoA for formation of curcumin, overview Curcuma longa