Application | Comment | Organism |
---|---|---|
synthesis | a curcuminoid producing unnatural fusion protein diketide-CoA synthase:curcumin synthase is constructed. The fusion protein may contribute to further understand the biosynthesis of curcuminoids in ginger but also be advantage to further manipulate the biosynthesis of curcuminoid analogs, particularly including tetrahydrobisdemethoxycurcumin (THBDC) and various dihydrocurcuminoid derivatives in microorganisms | Zingiber officinale |
Cloned (Comment) | Organism |
---|---|
diketide-CoA synthase, curcumin synthase, and the unnatural fusion protein diketide-CoA synthase:CURS His-tagged at their N terminus are produced in Escherichia coli BL21 (DE3) | Zingiber officinale |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a curcuminoid producing unnatural fusion protein diketide-CoA synthase:curcumin synthase is constructed | Zingiber officinale |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43240 | - |
SDS-PAGE | Zingiber officinale |
85470 | - |
diketide-CoA synthase:CURS His-tagged at their N terminus are produced in Escherichia coli BL21 (DE3), SDS-PAGE | Zingiber officinale |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zingiber officinale | A5GZV8 | - |
- |
Purification (Comment) | Organism |
---|---|
diketide-CoA synthase, curcumin synthase, and the unnatural fusion protein diketide-CoA synthase:curcumin synthase His-tagged at their N terminus are produced in Escherichia coli BL21 (DE3) | Zingiber officinale |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
feruloyl-CoA + feruloylacetyl-CoA + H2O | - |
Zingiber officinale | 2 CoA + curcumin + CO2 | - |
? | |
additional information | when diketide-CoA synthase (DCS) and curcumin synthase (ZoCURS) are co-incubated in the presence of 3-(4-hydroxyphenyl)propionyl-CoA and malonyl-CoA, tetrahydrobisdemethoxycurcumin and 4-hydroxybenzylacetone are produced | Zingiber officinale | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
ZoCURS | - |
Zingiber officinale |